FastQCFastQC Report
Wed 25 May 2016
SRR1781187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81304
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCA150.002217454870.0000115
ATAGTCC150.002217454870.0000156
TAGGATC150.002217454870.000015
GCGTCCC150.002217454870.0000119
GCACTTG150.002217454870.0000168
ACTTCGG200.006928940352.49999624
TCGATTC200.006928940352.49999647
AAGCGCG200.006928940352.4999967
GACTTGT200.006928940352.49999644
CTAACTA200.006928940352.49999642
GTTCGAT200.006928940352.49999645
TGGATTC200.006928940352.49999628
GGATTCT200.006928940352.49999629
GGTTCGA200.006928940352.49999644
TTCGATT200.006928940352.49999646
CTATACA350.001248899440.04
AATACCA350.001248899440.05
TGACAAC501.696569E-435.039
CCTATAC501.696569E-435.03
CATATTC400.002406278934.99999638
Produced by FastQC (version 0.11.5)