##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781187_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81304
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.477774771228965	34.0	34.0	34.0	31.0	34.0
2	32.59081963987012	34.0	34.0	34.0	31.0	34.0
3	32.5657778215094	34.0	34.0	34.0	31.0	34.0
4	32.64958673619994	34.0	34.0	34.0	31.0	34.0
5	32.66264882416609	34.0	34.0	34.0	31.0	34.0
6	35.85418921578274	38.0	37.0	38.0	32.0	38.0
7	35.79280232214897	38.0	37.0	38.0	31.0	38.0
8	35.69732116501033	38.0	37.0	38.0	31.0	38.0
9	35.868678047820524	38.0	37.0	38.0	32.0	38.0
10-11	35.7954590180065	38.0	37.0	38.0	31.5	38.0
12-13	35.74769384040145	38.0	37.0	38.0	31.0	38.0
14-15	35.68679277772311	38.0	37.0	38.0	31.0	38.0
16-17	35.65742152907606	38.0	37.0	38.0	31.0	38.0
18-19	35.67437026468562	38.0	37.0	38.0	31.0	38.0
20-21	35.63551608776936	38.0	37.0	38.0	31.0	38.0
22-23	35.6661787857916	38.0	37.0	38.0	31.0	38.0
24-25	35.64129071140411	38.0	37.0	38.0	31.0	38.0
26-27	35.6097977959264	38.0	37.0	38.0	31.0	38.0
28-29	35.67152292630129	38.0	37.0	38.0	31.0	38.0
30-31	35.71139181344091	38.0	37.0	38.0	31.0	38.0
32-33	35.658184099183316	38.0	37.0	38.0	31.0	38.0
34-35	35.68871765226803	38.0	37.0	38.0	31.0	38.0
36-37	35.61159967529272	38.0	37.0	38.0	31.0	38.0
38-39	35.60923816786382	38.0	37.0	38.0	31.0	38.0
40-41	35.60964405195317	38.0	37.0	38.0	31.0	38.0
42-43	35.658202548460096	38.0	37.0	38.0	31.0	38.0
44-45	35.68145478697235	38.0	37.0	38.0	31.0	38.0
46-47	35.66268572271967	38.0	37.0	38.0	31.0	38.0
48-49	35.63546688969792	38.0	37.0	38.0	31.0	38.0
50-51	35.558908540785204	38.0	37.0	38.0	31.0	38.0
52-53	35.60949645773886	38.0	37.0	38.0	31.0	38.0
54-55	35.59746137951392	38.0	37.0	38.0	31.0	38.0
56-57	35.52793835481649	38.0	37.0	38.0	31.0	38.0
58-59	35.53542876119256	38.0	37.0	38.0	31.0	38.0
60-61	35.51888590967234	38.0	37.0	38.0	31.0	38.0
62-63	35.47248597854964	38.0	36.0	38.0	31.0	38.0
64-65	35.46446669290563	38.0	36.0	38.0	31.0	38.0
66-67	35.36242374298928	38.0	36.0	38.0	30.0	38.0
68-69	35.06954762373315	38.0	36.0	38.0	27.5	38.0
70-71	35.27774156253075	38.0	36.0	38.0	28.5	38.0
72-73	35.26655515103808	38.0	36.0	38.0	28.0	38.0
74-75	35.279020712388075	38.0	36.0	38.0	28.5	38.0
76	34.01336957591263	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	11.0
14	32.0
15	112.0
16	156.0
17	243.0
18	309.0
19	337.0
20	297.0
21	278.0
22	318.0
23	361.0
24	380.0
25	501.0
26	585.0
27	766.0
28	846.0
29	1066.0
30	1400.0
31	1924.0
32	2437.0
33	3377.0
34	4770.0
35	7705.0
36	14134.0
37	38959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58083243136869	18.46895601692414	9.794106071042014	27.15610548066516
2	23.024697431860673	19.69152809209879	31.093181147299028	26.190593328741514
3	19.52794450457542	21.44420938699203	27.713273639673325	31.314572468759224
4	13.924284168060613	15.552740332578963	35.24672832824953	35.27624717111089
5	14.13337597166191	32.97500737971071	35.045016235363576	17.8466004132638
6	30.70697628652957	35.624323526517756	17.92285742398898	15.74584276296369
7	29.596329823870903	28.531191577290173	21.40116107448588	20.471317524353044
8	26.126635835875234	33.62688182623241	19.02489422414641	21.22158811374594
9	24.025878185575124	16.344829282692118	20.765276001180755	38.86401653055201
10-11	25.308717898258386	23.850610056085802	26.750836367214404	24.089835678441403
12-13	24.982165699104595	22.333464528190493	26.20350782249336	26.480861950211555
14-15	23.171676670274525	23.607079602479583	23.23501918724786	29.98622453999803
16-17	23.620609072124374	26.646290465413756	24.658073403522582	25.07502705893929
18-19	22.83897471219128	26.49070156449867	25.76318508314474	24.907138640165304
20-21	23.12186362294598	26.171529076060217	25.842516973334646	24.864090327659156
22-23	23.23378923546197	25.575002459903573	26.346182229656595	24.84502607497786
24-25	22.91092689166585	25.986421332283776	26.115566269802226	24.987085506248157
26-27	22.406031683558005	26.136475450162354	26.296369182328057	25.16112368395159
28-29	22.951515300600217	26.210272557315754	26.791424776148776	24.046787365935256
30-31	23.179056380989866	25.956287513529468	26.253320869821906	24.611335235658764
32-33	22.731968906818853	26.2723851225032	26.110646462658664	24.884999508019284
34-35	23.431196497097314	25.70722227688675	26.313588507330515	24.54799271868543
36-37	23.26576798189511	25.804388467972057	25.972276886746037	24.957566663386796
38-39	22.50258289875037	26.4691774082456	26.518990455574144	24.50924923742989
40-41	22.863573747909083	25.654334350093478	26.916879858309557	24.565212043687886
42-43	22.600364065728623	25.614975892944997	26.99252189314179	24.79213814818459
44-45	22.233838433533407	25.825297648332185	26.820328643117193	25.120535275017218
46-47	22.322394962117485	25.252140116107448	27.174554757453507	25.25091016432156
48-49	22.014292039752043	25.29395847682771	27.538620486076947	25.153128997343305
50-51	21.567819541473973	25.506740135786675	27.58228377447604	25.343156548263305
52-53	21.613327757551904	25.203557020564794	28.01399685132343	25.169118370559872
54-55	20.79540981993506	25.52580438846797	28.30549542457936	25.373290367017614
56-57	20.83107842172587	25.786554167076652	28.087793958476826	25.294573452720652
58-59	21.2388074387484	25.7066073009938	27.56014464233002	25.494440617927776
60-61	21.012496310144645	25.31117780183017	27.807364951293913	25.868960936731277
62-63	20.66380497884483	25.20294204467185	28.08656400669094	26.046688969792385
64-65	21.306454786972353	25.429868149168556	27.43161468070452	25.83206238315458
66-67	21.304609859293517	24.90406376070058	27.106292433336616	26.68503394666929
68-69	21.31014464233002	24.870240086588606	26.87998130473285	26.93963396634852
70-71	22.076404604939487	24.9016038571288	26.910730099380103	26.111261438551608
72-73	22.392502213913215	24.579356489225624	26.144470136770636	26.883671160090522
74-75	22.715979533602283	24.5572173570796	26.045459018006493	26.681344091311622
76	23.51667814621667	24.178392207025485	25.53871888221982	26.76621076453803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	4.0
14	6.0
15	8.5
16	16.5
17	20.0
18	30.5
19	51.0
20	82.0
21	103.0
22	119.5
23	183.5
24	297.0
25	363.0
26	389.0
27	492.5
28	684.5
29	799.0
30	855.0
31	993.5
32	1312.5
33	1549.0
34	1671.0
35	2006.0
36	2457.5
37	2696.0
38	2865.5
39	3377.5
40	3978.0
41	4423.0
42	4610.0
43	4529.0
44	4532.0
45	4531.0
46	4446.0
47	4416.5
48	4208.5
49	3832.0
50	3634.0
51	3328.0
52	2787.0
53	2371.0
54	2190.0
55	1963.0
56	1632.0
57	1407.5
58	1287.0
59	1220.0
60	1062.5
61	956.0
62	940.0
63	950.0
64	932.5
65	963.0
66	1037.0
67	1053.0
68	1035.0
69	1009.5
70	941.5
71	881.0
72	901.0
73	840.0
74	742.5
75	726.0
76	660.5
77	576.5
78	494.5
79	431.0
80	377.0
81	266.0
82	176.0
83	143.0
84	121.0
85	81.5
86	48.5
87	33.0
88	28.0
89	18.5
90	11.5
91	5.5
92	2.0
93	2.0
94	1.5
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	81304.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.646364262520905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55071268700672	47.329774672832826
2	13.792358738759964	17.2808225917544
3	4.372324969568461	8.217307881531044
4	1.9908116385911179	4.988684443569812
5	1.2035182785565635	3.7698022237528286
6	0.7382102328503554	2.7747712289678246
7	0.6066674519967016	2.660385712880055
8	0.42211489378411277	2.115517071730788
9	0.28075548749361917	1.5829479484404212
>10	1.0385989712176542	9.12501229951786
>50	0.003926650174735933	0.15497392502213914
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.001229951785889993	0.0
6	0.0	0.0	0.0	0.001229951785889993	0.0
7	0.0	0.0	0.0	0.001229951785889993	0.0
8	0.0	0.0	0.0	0.001229951785889993	0.0
9	0.0	0.0	0.0	0.002459903571779986	0.0
10	0.0	0.0	0.0	0.002459903571779986	0.0
11	0.0	0.0	0.0	0.002459903571779986	0.0
12	0.0	0.0	0.0	0.002459903571779986	0.0
13	0.0	0.0	0.0	0.002459903571779986	0.0
14	0.0	0.0	0.0	0.002459903571779986	0.0
15	0.0	0.0	0.0	0.002459903571779986	0.0
16	0.0	0.0	0.0	0.002459903571779986	0.0
17	0.0	0.0	0.0	0.002459903571779986	0.0
18	0.0	0.0	0.0	0.002459903571779986	0.0
19	0.0	0.0	0.0	0.004919807143559972	0.0
20	0.0	0.0	0.0	0.004919807143559972	0.0
21	0.0	0.0	0.0	0.006149758929449966	0.0
22	0.0	0.0	0.0	0.008609662501229952	0.0
23	0.0	0.0	0.0	0.009839614287119944	0.0
24	0.0	0.0	0.0	0.011069566073009938	0.0
25	0.0	0.0	0.0	0.012299517858899931	0.0
26	0.0	0.0	0.0	0.012299517858899931	0.0
27	0.0	0.0	0.0	0.018449276788349896	0.0
28	0.0	0.0	0.0	0.03197874643313982	0.0
29	0.0	0.0	0.0	0.05165797500737971	0.0
30	0.0	0.0	0.0	0.06395749286627964	0.0
31	0.0	0.0	0.0	0.07625701072517957	0.0
32	0.0	0.0	0.0	0.09224638394174949	0.0
33	0.0	0.0	0.0	0.12053527501721932	0.0
34	0.0	0.0	0.0	0.17342320181048904	0.0
35	0.0	0.0	0.0	0.24107055003443864	0.0
36	0.0	0.0	0.0	0.3677555839811079	0.0
37	0.0	0.0	0.0	0.5633179179376169	0.0
38	0.0	0.0	0.0	0.8462068286923152	0.0
39	0.0	0.0	0.0	1.1561546787365935	0.0
40	0.0	0.0	0.0	1.4661025287808718	0.0
41	0.0	0.0	0.0	1.8793663288399094	0.0
42	0.0	0.0	0.0	2.2704909967529274	0.0
43	0.0	0.0	0.0	2.795680409327954	0.0
44	0.0	0.0	0.0	3.389747121912821	0.0
45	0.0	0.0	0.0	4.103119157729017	0.0
46	0.0	0.0	0.0	4.825100856046443	0.0
47	0.0	0.0	0.0	5.627029420446719	0.0
48	0.0	0.0	0.0	6.282593722326085	0.0
49	0.0	0.0	0.0	6.954147397422021	0.0
50	0.0	0.0	0.0	7.626931024303848	0.0
51	0.0	0.0	0.0	8.303404506543343	0.0
52	0.0	0.0	0.0	8.87287218341041	0.0
53	0.0	0.0	0.0	9.473088654924727	0.0
54	0.0	0.0	0.0	10.166781462166684	0.0
55	0.0	0.0	0.0	10.84448489619207	0.0
56	0.0	0.0	0.0	11.576306208796614	0.0
57	0.0	0.0	0.0	12.25769949817967	0.0
58	0.0	0.0	0.0	12.908343992915478	0.0
59	0.0	0.0	0.0	13.528239693004034	0.0
60	0.0	0.0	0.0	14.22439240381777	0.0
61	0.0	0.0	0.0	14.856587621765227	0.0
62	0.0	0.0	0.0	15.467873659352554	0.0
63	0.0	0.0	0.0	16.16156646659451	0.0
64	0.0	0.0	0.0	16.794991636327858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCCA	15	0.0022174548	70.00001	15
ATAGTCC	15	0.0022174548	70.00001	56
TAGGATC	15	0.0022174548	70.00001	5
GCGTCCC	15	0.0022174548	70.00001	19
GCACTTG	15	0.0022174548	70.00001	68
ACTTCGG	20	0.0069289403	52.499996	24
TCGATTC	20	0.0069289403	52.499996	47
AAGCGCG	20	0.0069289403	52.499996	7
GACTTGT	20	0.0069289403	52.499996	44
CTAACTA	20	0.0069289403	52.499996	42
GTTCGAT	20	0.0069289403	52.499996	45
TGGATTC	20	0.0069289403	52.499996	28
GGATTCT	20	0.0069289403	52.499996	29
GGTTCGA	20	0.0069289403	52.499996	44
TTCGATT	20	0.0069289403	52.499996	46
CTATACA	35	0.0012488994	40.0	4
AATACCA	35	0.0012488994	40.0	5
TGACAAC	50	1.696569E-4	35.0	39
CCTATAC	50	1.696569E-4	35.0	3
CATATTC	40	0.0024062789	34.999996	38
>>END_MODULE