FastQCFastQC Report
Wed 25 May 2016
SRR1781187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79515
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT5860.7369678676979187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1250.058.838
CTCGTAT1500.058.33333239
CGTATGC1600.056.87541
TATGCCG1600.056.87543
GTATGCC1600.056.87542
GCCGTCT1600.056.87546
ATCTCGT1300.056.53846437
CGTCTTC1550.056.4516148
CCGTCTT1550.056.4516147
TCGTATG1550.056.4516140
CACGAGC252.377477E-456.00000421
ATATAAT200.00692837852.553
CTTGAAA1600.052.557
ATGGTCC200.00692837852.58
TGCTACC200.00692837852.538
ACAAGTG200.00692837852.527
ACGGTTA200.00692837852.550
ATGCCGT1800.052.49999644
CATCTCG1500.051.33333236
TGCCGTC1850.051.0810845
Produced by FastQC (version 0.11.5)