FastQCFastQC Report
Wed 25 May 2016
SRR1781186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG290.34918723660445516No Hit
TTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGA140.16857314870559903No Hit
CCCTAGGAGGGCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT140.16857314870559903No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC130.15653220951234195No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA130.15653220951234195No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA120.14449127031908487No Hit
CTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAA120.14449127031908487No Hit
GCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACC100.12040939193257075No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC100.12040939193257075No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA100.12040939193257075No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT90.10836845273931367No Hit
CTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAATTACAATATATA90.10836845273931367No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA90.10836845273931367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC207.474791E-570.07
GAGAACC150.002147682270.056
CTTTACA150.002147682270.070
AGCTAAC150.002147682270.019
CACTGTA150.002147682270.011
GAGCCCA150.002147682270.06
CCCACTG150.002147682270.09
AACTTAG150.002147682270.023
CCACTGT150.002147682270.010
TAACCTT150.002147682270.033
AAGATTA150.002147682270.048
CTAACTT150.002147682270.021
GAACCAA150.002147682270.058
GCTAACT150.002147682270.020
ATTAAGA150.002147682270.051
TTAAGAG150.002147682270.052
AGAGAAC150.002147682270.055
ACCTCTT150.002147682270.066
ACCTTTT150.002147682270.035
TAGAGCC150.002147682270.04
Produced by FastQC (version 0.11.5)