##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781186_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8305
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953281155930163	34.0	34.0	34.0	27.0	34.0
2	32.32209512341963	34.0	34.0	34.0	31.0	34.0
3	32.23419626730885	34.0	34.0	34.0	30.0	34.0
4	32.42191450933173	34.0	34.0	34.0	31.0	34.0
5	32.31547260686334	34.0	34.0	34.0	31.0	34.0
6	35.53281155930163	38.0	37.0	38.0	31.0	38.0
7	35.47850692354004	38.0	37.0	38.0	31.0	38.0
8	35.3471402769416	38.0	37.0	38.0	28.0	38.0
9	35.61444912703191	38.0	37.0	38.0	31.0	38.0
10-11	35.54569536423841	38.0	37.0	38.0	31.0	38.0
12-13	35.54954846478025	38.0	37.0	38.0	31.0	38.0
14-15	35.477844671884405	38.0	37.0	38.0	29.5	38.0
16-17	35.48151715833835	38.0	37.0	38.0	31.0	38.0
18-19	35.39042745334136	38.0	37.0	38.0	30.5	38.0
20-21	35.37134256472005	38.0	37.0	38.0	29.0	38.0
22-23	35.3939193257074	38.0	37.0	38.0	29.0	38.0
24-25	35.406863335340155	38.0	37.0	38.0	29.0	38.0
26-27	35.4271523178808	38.0	37.0	38.0	31.0	38.0
28-29	35.42986152919927	38.0	37.0	38.0	30.0	38.0
30-31	35.50307043949428	38.0	37.0	38.0	31.0	38.0
32-33	35.45316074653823	38.0	37.0	38.0	31.0	38.0
34-35	35.49066827212523	38.0	37.0	38.0	31.0	38.0
36-37	35.464720048163755	38.0	37.0	38.0	31.0	38.0
38-39	35.44069837447321	38.0	37.0	38.0	29.5	38.0
40-41	35.43762793497893	38.0	37.0	38.0	31.0	38.0
42-43	35.4539434075858	38.0	37.0	38.0	31.0	38.0
44-45	35.47712221553281	38.0	37.0	38.0	31.0	38.0
46-47	35.45111378687538	38.0	37.0	38.0	30.5	38.0
48-49	35.44810355207706	38.0	37.0	38.0	31.0	38.0
50-51	35.31426851294401	38.0	37.0	38.0	27.5	38.0
52-53	35.38380493678507	38.0	37.0	38.0	30.0	38.0
54-55	35.32745334136062	38.0	37.0	38.0	27.5	38.0
56-57	35.33377483443709	38.0	37.0	38.0	29.0	38.0
58-59	35.319626730885005	38.0	37.0	38.0	27.5	38.0
60-61	35.307405177603854	38.0	37.0	38.0	27.5	38.0
62-63	35.23925346177002	38.0	37.0	38.0	27.0	38.0
64-65	35.22606863335341	38.0	37.0	38.0	27.0	38.0
66-67	35.18314268512944	38.0	37.0	38.0	27.0	38.0
68-69	34.96568332329922	38.0	36.0	38.0	26.0	38.0
70-71	35.12913907284768	38.0	36.5	38.0	27.0	38.0
72-73	35.195785671282366	38.0	37.0	38.0	27.0	38.0
74-75	35.08103552077062	38.0	36.5	38.0	27.0	38.0
76	34.16207104154124	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	14.0
15	20.0
16	27.0
17	49.0
18	67.0
19	63.0
20	83.0
21	48.0
22	48.0
23	60.0
24	51.0
25	56.0
26	54.0
27	68.0
28	86.0
29	97.0
30	105.0
31	165.0
32	200.0
33	257.0
34	365.0
35	537.0
36	1176.0
37	4605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.98916315472607	18.62733293196869	10.307043949428056	33.076459963877184
2	19.63877182420229	19.98795906080674	32.0048163756773	28.368452739313664
3	17.80854906682721	21.420830824804334	27.886815171583386	32.88380493678507
4	12.2576760987357	16.026490066225165	34.84647802528597	36.86935580975316
5	12.751354605659241	32.60686333534016	34.73810957254666	19.903672486453942
6	25.55087296809151	37.73630343166767	18.952438290186635	17.760385310054183
7	25.97230583985551	30.054184226369657	22.022877784467187	21.950632149307648
8	22.829620710415412	34.37688139674895	19.01264298615292	23.780854906682723
9	21.02347983142685	15.568934376881396	20.096327513546054	43.3112582781457
10-11	21.733895243829018	24.485249849488262	27.062010836845275	26.71884406983745
12-13	21.721854304635762	23.46779048765804	25.454545454545453	29.355809753160745
14-15	20.722456351595426	23.521974714027692	23.564118001204093	32.19145093317279
16-17	20.854906682721253	28.22396146899458	24.087898856110776	26.83323299217339
18-19	20.475617098133654	28.007224563515955	24.683925346177002	26.83323299217339
20-21	19.67489464178206	27.31487055990367	25.285972305839856	27.72426249247441
22-23	19.903672486453942	27.29078868151716	25.89403973509934	26.91149909692956
24-25	20.33112582781457	27.429259482239615	25.34617700180614	26.893437688139677
26-27	19.75918121613486	26.83323299217339	26.724864539434073	26.682721252257675
28-29	19.10295003010235	27.838651414810357	26.971703792895845	26.086694762191453
30-31	20.301023479831425	27.37507525586996	26.387718242022878	25.93618302227574
32-33	19.10295003010235	27.60385310054184	27.068031306441902	26.225165562913904
34-35	19.735099337748345	27.501505117399155	26.29139072847682	26.472004816375676
36-37	19.67489464178206	26.929560505719447	25.93618302227574	27.459361830222758
38-39	19.717037928958458	27.12823600240819	27.41119807344973	25.743527995183623
40-41	19.729078868151717	26.67068031306442	26.923540036122816	26.67670078266105
42-43	19.30162552679109	27.57977122215533	26.42986152919928	26.688741721854303
44-45	18.771824202287778	27.435279951836243	26.887417218543046	26.90547862733293
46-47	19.096929560505718	27.393136664659846	26.86935580975316	26.640577965081274
48-49	18.946417820590007	28.079470198675498	26.67068031306442	26.30343166767008
50-51	19.58458759783263	28.531005418422637	25.85189644792294	26.032510535821796
52-53	18.741721854304636	27.18844069837447	25.821794099939794	28.248043347381095
54-55	19.50632149307646	27.808549066827215	26.32751354605659	26.35761589403974
56-57	19.253461770018063	27.68813967489464	26.31547260686333	26.742925948223963
58-59	19.963877182420227	28.13365442504515	25.111378687537627	26.79108970499699
60-61	18.741721854304636	27.573750752558702	26.646598434677905	27.037928958458764
62-63	18.500903070439495	27.12823600240819	27.092113184828413	27.2787477423239
64-65	19.41601444912703	28.07344972907887	26.189042745334135	26.321493076459962
66-67	19.29560505719446	27.573750752558702	26.79711017459362	26.33353401565322
68-69	18.169777242624924	27.760385310054186	26.887417218543046	27.182420228777843
70-71	19.271523178807946	27.37507525586996	27.049969897652016	26.30343166767008
72-73	19.33172787477423	27.043949428055384	26.779048765803733	26.84527393136665
74-75	19.6628537025888	26.893437688139677	26.88139674894642	26.562311860325106
76	19.939795304033716	27.043949428055384	26.29741119807345	26.71884406983745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	1.0
17	2.0
18	4.5
19	7.0
20	9.5
21	12.0
22	13.0
23	18.0
24	21.0
25	20.0
26	25.5
27	36.5
28	60.5
29	79.0
30	82.0
31	91.5
32	119.0
33	140.0
34	160.0
35	230.0
36	272.0
37	264.0
38	308.0
39	367.5
40	446.5
41	511.0
42	512.0
43	497.5
44	495.5
45	499.5
46	491.0
47	493.0
48	460.5
49	441.0
50	456.0
51	423.0
52	328.0
53	253.5
54	241.0
55	219.5
56	173.5
57	142.5
58	136.0
59	121.5
60	92.0
61	84.5
62	92.0
63	78.5
64	60.0
65	61.5
66	63.5
67	59.0
68	68.5
69	69.0
70	60.0
71	60.0
72	51.5
73	46.5
74	47.0
75	44.0
76	45.5
77	39.5
78	26.5
79	21.0
80	17.0
81	15.5
82	15.0
83	12.0
84	12.0
85	7.0
86	3.5
87	5.0
88	4.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	8305.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.89343768813967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.25806451612904	63.25105358217941
2	11.916692765424365	18.326309452137266
3	3.1005324146570623	7.152317880794702
4	1.3310366426558096	4.093919325707406
5	0.6733479486376448	2.588801926550271
6	0.281866583150642	1.300421432871764
7	0.17225180081428124	0.9271523178807948
8	0.06263701847792046	0.38531005418422637
9	0.04697776385844034	0.325105358217941
>10	0.15659254619480112	1.6496086694762193
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG	29	0.34918723660445516	No Hit
TTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGA	14	0.16857314870559903	No Hit
CCCTAGGAGGGCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT	14	0.16857314870559903	No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC	13	0.15653220951234195	No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA	13	0.15653220951234195	No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA	12	0.14449127031908487	No Hit
CTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAA	12	0.14449127031908487	No Hit
GCACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACC	10	0.12040939193257075	No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC	10	0.12040939193257075	No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA	10	0.12040939193257075	No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT	9	0.10836845273931367	No Hit
CTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAATTACAATATATA	9	0.10836845273931367	No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA	9	0.10836845273931367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.012040939193257074	0.0
22	0.0	0.0	0.0	0.060204695966285374	0.0
23	0.0	0.0	0.0	0.060204695966285374	0.0
24	0.0	0.0	0.0	0.060204695966285374	0.0
25	0.0	0.0	0.0	0.060204695966285374	0.0
26	0.0	0.0	0.0	0.060204695966285374	0.0
27	0.0	0.0	0.0	0.060204695966285374	0.0
28	0.0	0.0	0.0	0.060204695966285374	0.0
29	0.0	0.0	0.0	0.060204695966285374	0.0
30	0.0	0.0	0.0	0.060204695966285374	0.0
31	0.0	0.0	0.0	0.060204695966285374	0.0
32	0.0	0.0	0.0	0.060204695966285374	0.0
33	0.0	0.0	0.0	0.060204695966285374	0.0
34	0.0	0.0	0.0	0.07224563515954245	0.0
35	0.0	0.0	0.0	0.07224563515954245	0.0
36	0.0	0.0	0.0	0.08428657435279951	0.0
37	0.0	0.0	0.0	0.13245033112582782	0.0
38	0.0	0.0	0.0	0.1806140878988561	0.0
39	0.0	0.0	0.0	0.20469596628537026	0.0
40	0.0	0.0	0.0	0.26490066225165565	0.0
41	0.0	0.0	0.0	0.3130644190246839	0.0
42	0.0	0.0	0.0	0.3973509933774834	0.0
43	0.0	0.0	0.0	0.5177603853100542	0.0
44	0.0	0.0	0.0	0.5659241420830825	0.0
45	0.0	0.0	0.0	0.6261288380493678	0.0
46	0.0	0.0	0.0	0.6863335340156532	0.0
47	0.0	0.0	0.0	0.8308248043347382	0.0
48	0.0	0.0	0.0	0.8669476219145094	0.0
49	0.0	0.0	0.0	0.9271523178807947	0.0
50	0.0	0.0	0.0	1.0596026490066226	0.0
51	0.0	0.0	0.0	1.131848284166165	0.0
52	0.0	0.0	0.0	1.2522576760987356	0.0
53	0.0	0.0	0.0	1.3726670680313064	0.0
54	0.0	0.0	0.0	1.5051173991571343	0.0
55	0.0	0.0	0.0	1.6134858518964479	0.0
56	0.0	0.0	0.0	1.77001806140879	0.0
57	0.0	0.0	0.0	1.9024683925346177	0.0
58	0.0	0.0	0.0	1.9867549668874172	0.0
59	0.0	0.0	0.0	2.0590006020469596	0.0
60	0.0	0.0	0.0	2.1794099939795304	0.0
61	0.0	0.0	0.0	2.275737507525587	0.0
62	0.0	0.0	0.0	2.3720650210716436	0.0
63	0.0	0.0	0.0	2.4924744130042145	0.0
64	0.0	0.0	0.0	2.5767609873570136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	20	7.474791E-5	70.0	7
GAGAACC	15	0.0021476822	70.0	56
CTTTACA	15	0.0021476822	70.0	70
AGCTAAC	15	0.0021476822	70.0	19
CACTGTA	15	0.0021476822	70.0	11
GAGCCCA	15	0.0021476822	70.0	6
CCCACTG	15	0.0021476822	70.0	9
AACTTAG	15	0.0021476822	70.0	23
CCACTGT	15	0.0021476822	70.0	10
TAACCTT	15	0.0021476822	70.0	33
AAGATTA	15	0.0021476822	70.0	48
CTAACTT	15	0.0021476822	70.0	21
GAACCAA	15	0.0021476822	70.0	58
GCTAACT	15	0.0021476822	70.0	20
ATTAAGA	15	0.0021476822	70.0	51
TTAAGAG	15	0.0021476822	70.0	52
AGAGAAC	15	0.0021476822	70.0	55
ACCTCTT	15	0.0021476822	70.0	66
ACCTTTT	15	0.0021476822	70.0	35
TAGAGCC	15	0.0021476822	70.0	4
>>END_MODULE