FastQCFastQC Report
Wed 25 May 2016
SRR1781186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7554
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT130.17209425469949696Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT120.15885623510722796No Hit
TAGTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGG100.13238019592268996No Hit
GCCTAGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTT100.13238019592268996No Hit
ATGATAGGCATGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG80.10590415673815197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAATC200.006688862652.566
AAAAAAT200.006688862652.565
Produced by FastQC (version 0.11.5)