##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781186_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7554
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835451416468096	34.0	32.0	34.0	27.0	34.0
2	32.39290442149854	34.0	34.0	34.0	27.0	34.0
3	32.71789780248875	34.0	34.0	34.0	31.0	34.0
4	32.952475509663756	34.0	34.0	34.0	31.0	34.0
5	32.70916070955785	34.0	34.0	34.0	31.0	34.0
6	35.66944665078104	37.0	36.0	38.0	31.0	38.0
7	35.66521048451152	37.0	36.0	38.0	31.0	38.0
8	35.16296002118083	37.0	35.0	38.0	27.0	38.0
9	35.39687582737623	37.0	35.0	38.0	31.0	38.0
10-11	35.23610007942811	37.0	35.0	38.0	30.5	38.0
12-13	35.207439767010854	37.0	35.0	38.0	29.0	38.0
14-15	35.27713794016415	37.0	35.0	38.0	30.5	38.0
16-17	35.16739475774424	37.0	35.0	38.0	27.0	38.0
18-19	35.248411437648926	37.0	35.0	38.0	31.0	38.0
20-21	35.131519724649195	37.0	35.0	38.0	27.0	38.0
22-23	35.12926926131851	37.0	35.0	38.0	27.0	38.0
24-25	35.130328302885886	37.0	35.0	38.0	27.0	38.0
26-27	34.85980937251787	37.0	34.5	38.0	27.0	38.0
28-29	34.869473126820225	37.0	34.0	38.0	27.0	38.0
30-31	34.94499602859412	37.0	34.5	38.0	27.0	38.0
32-33	34.84868943606037	37.0	34.0	38.0	27.0	38.0
34-35	34.75006619009796	37.0	34.0	38.0	27.0	38.0
36-37	34.64714058776807	37.0	34.0	38.0	27.0	38.0
38-39	34.78594122319301	37.0	34.0	38.0	27.0	38.0
40-41	34.6799708763569	37.0	34.0	38.0	27.0	38.0
42-43	34.59696849351337	37.0	34.0	38.0	27.0	38.0
44-45	34.64019062748213	37.0	34.0	38.0	27.0	38.0
46-47	34.61139793486895	37.0	34.0	38.0	26.5	38.0
48-49	34.595909451945985	37.0	34.0	38.0	27.0	38.0
50-51	34.634299708763564	37.0	34.0	38.0	27.0	38.0
52-53	34.47709822610537	37.0	34.0	38.0	26.5	38.0
54-55	34.4361265554673	37.0	34.0	38.0	26.0	38.0
56-57	34.438244638602065	37.0	34.0	38.0	26.0	38.0
58-59	34.44612126025947	37.0	34.0	38.0	26.5	38.0
60-61	34.39230871061689	37.0	34.0	38.0	26.0	38.0
62-63	34.34385755890919	37.0	34.0	38.0	25.0	38.0
64-65	34.25152237225311	37.0	34.0	38.0	24.5	38.0
66-67	34.26052422557585	37.0	34.0	38.0	25.0	38.0
68-69	34.246227164416204	37.0	34.0	38.0	24.5	38.0
70-71	34.22511252316653	37.0	34.0	38.0	25.0	38.0
72-73	34.184207042626426	37.0	34.0	38.0	24.0	38.0
74-75	34.22809107757479	37.0	34.0	38.0	24.5	38.0
76	33.15369340746624	37.0	32.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	1.0
23	11.0
24	13.0
25	33.0
26	51.0
27	95.0
28	144.0
29	210.0
30	297.0
31	435.0
32	544.0
33	705.0
34	930.0
35	1171.0
36	1436.0
37	1474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.516282764098484	20.20121789780249	9.10775747948107	20.17474185861795
2	27.813079163357163	24.15938575589092	27.852793222133972	20.17474185861795
3	24.040243579560496	24.66243050039714	27.614508869473127	23.682817050569234
4	18.109610802223987	19.817315329626688	36.12655546730209	25.946518400847236
5	18.5729414879534	34.25999470479216	32.777336510458035	14.389727296796398
6	32.72438443208896	36.40455387873974	18.109610802223987	12.761450886947312
7	31.771247021445593	29.414879534021708	22.319301032565527	16.49457241196717
8	28.10431559438708	34.98808578236696	19.55255493778131	17.355043685464654
9	28.713264495631453	18.294943076515754	21.2205454064072	31.771247021445593
10-11	28.88535875033095	25.033095048980673	26.773894625364047	19.307651575324332
12-13	28.92507280910776	22.74953666931427	26.317182949430766	22.008207572147207
14-15	26.363516018003708	24.894095843261848	24.106433677521842	24.635954461212602
16-17	26.57532433148001	26.98570293884035	25.35742652899126	21.08154620068838
18-19	25.70823404818639	26.515753243314798	26.376754037595973	21.39925867090283
20-21	26.21127879269261	26.648133439237494	26.330420969023034	20.81016679904686
22-23	26.356897008207575	26.595181360868413	26.634895419645222	20.413026211278794
24-25	26.303944929838497	26.52899126290707	26.846703733121522	20.32036007413291
26-27	25.628805930632776	27.581413820492457	26.555467302091607	20.234312946783163
28-29	25.92666137145883	25.906804342070426	27.832936192745567	20.33359809372518
30-31	25.529520783690764	25.575853852263702	27.965316388668253	20.929308975377285
32-33	25.476568705321682	27.773365104580357	26.760656605771775	19.989409584326186
34-35	26.01932750860471	26.965845909451946	26.449563145353455	20.565263436589888
36-37	26.3238019592269	26.171564733915808	27.091607095578503	20.413026211278794
38-39	26.125231665342863	26.376754037595973	27.409319565792956	20.088694731268202
40-41	25.886947312682025	26.151707704527404	27.95869737887212	20.00264760391845
42-43	26.23775483187715	26.131850675139003	27.6476039184538	19.98279057453005
44-45	24.536669314270583	26.37013502779984	27.621127879269263	21.47206777866031
46-47	25.946518400847236	26.198040773100345	27.171035213132118	20.684405612920305
48-49	25.549377813079165	25.80751919512841	28.362456976436324	20.2806460153561
50-51	25.906804342070426	26.608419380460685	27.574794810696318	19.90998146677257
52-53	26.204659782896478	26.82022769393699	26.965845909451946	20.009266613714587
54-55	25.430235636748744	25.64866296002118	27.79984114376489	21.12126025946518
56-57	25.29123643102992	25.569234842467566	28.124172623775483	21.015356102727033
58-59	25.370664548583534	26.740799576383374	26.992321948636484	20.896213926396612
60-61	24.841143764892774	27.098226105374636	27.786603124172625	20.27402700555997
62-63	24.417527137940166	27.23060630129733	28.481599152766744	19.870267407995765
64-65	25.377283558379666	26.694466507810432	27.098226105374636	20.830023828435266
66-67	25.66190097961345	27.03203600741329	26.555467302091607	20.750595710881655
68-69	24.17262377548319	27.243844320889593	28.0315064866296	20.55202541699762
70-71	24.563145353455123	27.051893036801694	27.766746094784224	20.618215514958962
72-73	25.628805930632776	25.45671167593328	28.024887476833467	20.889594916600476
74-75	24.834524755096638	26.416468096372782	28.0315064866296	20.717500661900978
76	26.806989674344717	26.092136616362193	26.23775483187715	20.86311887741594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	0.5
17	0.0
18	3.5
19	6.5
20	5.0
21	4.0
22	6.0
23	16.0
24	28.0
25	32.0
26	29.0
27	34.0
28	52.0
29	62.0
30	77.0
31	99.5
32	133.5
33	160.0
34	164.0
35	195.0
36	239.0
37	256.0
38	272.0
39	323.0
40	374.0
41	413.0
42	436.0
43	476.0
44	520.0
45	500.0
46	476.0
47	490.5
48	472.5
49	388.0
50	336.0
51	320.0
52	260.0
53	216.5
54	217.0
55	191.0
56	154.0
57	121.5
58	100.0
59	98.0
60	87.5
61	64.5
62	50.0
63	54.5
64	63.0
65	65.5
66	61.0
67	58.0
68	60.0
69	58.5
70	51.0
71	47.0
72	44.0
73	50.0
74	50.0
75	41.0
76	35.5
77	31.5
78	32.5
79	32.0
80	24.5
81	16.0
82	10.0
83	5.0
84	6.5
85	4.5
86	0.5
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	7554.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.79163357161768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.46665574307718	68.24199099814668
2	10.84712436506636	17.52713794016415
3	2.8674422415205636	6.949960285941223
4	0.8848107488120597	2.8594122319301034
5	0.5898738325413732	2.3828435266084194
6	0.180239226609864	0.8737092930897538
7	0.08192692118630182	0.46333068572941494
8	0.016385384237260363	0.10590415673815197
9	0.0	0.0
>10	0.06554153694904145	0.5957108816521048
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT	13	0.17209425469949696	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	12	0.15885623510722796	No Hit
TAGTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGG	10	0.13238019592268996	No Hit
GCCTAGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTT	10	0.13238019592268996	No Hit
ATGATAGGCATGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG	8	0.10590415673815197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.013238019592268996	0.0
20	0.0	0.0	0.0	0.013238019592268996	0.0
21	0.0	0.0	0.0	0.013238019592268996	0.0
22	0.0	0.0	0.0	0.013238019592268996	0.0
23	0.0	0.0	0.0	0.013238019592268996	0.0
24	0.0	0.0	0.0	0.013238019592268996	0.0
25	0.0	0.0	0.0	0.013238019592268996	0.0
26	0.0	0.0	0.0	0.013238019592268996	0.0
27	0.0	0.0	0.0	0.013238019592268996	0.0
28	0.0	0.0	0.0	0.013238019592268996	0.0
29	0.0	0.0	0.0	0.013238019592268996	0.0
30	0.0	0.0	0.0	0.013238019592268996	0.0
31	0.0	0.0	0.0	0.013238019592268996	0.0
32	0.0	0.0	0.0	0.026476039184537992	0.0
33	0.0	0.0	0.0	0.026476039184537992	0.0
34	0.0	0.0	0.0	0.026476039184537992	0.0
35	0.0	0.0	0.0	0.03971405877680699	0.0
36	0.0	0.0	0.0	0.052952078369075985	0.0
37	0.0	0.0	0.0	0.14561821551495896	0.0
38	0.0	0.0	0.0	0.18533227429176596	0.0
39	0.0	0.0	0.0	0.19857029388403494	0.0
40	0.0	0.0	0.0	0.2515223722531109	0.0
41	0.0	0.0	0.0	0.3309504898067249	0.0
42	0.0	0.0	0.0	0.3839025681758009	0.0
43	0.0	0.0	0.0	0.5030447445062218	0.0
44	0.0	0.0	0.0	0.5692348424675668	0.0
45	0.0	0.0	0.0	0.6221869208366428	0.0
46	0.0	0.0	0.0	0.6883770187979878	0.0
47	0.0	0.0	0.0	0.8075191951284088	0.0
48	0.0	0.0	0.0	0.8339952343129468	0.0
49	0.0	0.0	0.0	0.8869473126820228	0.0
50	0.0	0.0	0.0	1.0590415673815197	0.0
51	0.0	0.0	0.0	1.1384696849351337	0.0
52	0.0	0.0	0.0	1.2311358220810167	0.0
53	0.0	0.0	0.0	1.3635160180037067	0.0
54	0.0	0.0	0.0	1.4958962139263967	0.0
55	0.0	0.0	0.0	1.6018003706645485	0.0
56	0.0	0.0	0.0	1.7474185861795075	0.0
57	0.0	0.0	0.0	1.9195128408790045	0.0
58	0.0	0.0	0.0	1.9724649192480805	0.0
59	0.0	0.0	0.0	2.0651310563939633	0.0
60	0.0	0.0	0.0	2.2107492719089223	0.0
61	0.0	0.0	0.0	2.3034154090548054	0.0
62	0.0	0.0	0.0	2.3960815462006884	0.0
63	0.0	0.0	0.0	2.5019857029388404	0.0
64	0.0	0.0	0.0	2.6078898596769924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAATC	20	0.0066888626	52.5	66
AAAAAAT	20	0.0066888626	52.5	65
>>END_MODULE