FastQCFastQC Report
Wed 25 May 2016
SRR1781185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133751
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAG150.002220597170.03
CGTTGGT200.0069386852.50000414
TTAGCCA305.860687E-446.6666644
GTAATTG305.860687E-446.6666648
CGATTAG459.1583584E-538.8888952
TTGCGAT459.1583584E-538.8888949
TTCGGTA459.1583584E-538.8888926
GTAAGCA459.1583584E-538.8888930
CGGTAAG459.1583584E-538.8888928
TCGTTCG501.7024306E-435.023
GTTCGGT501.7024306E-435.025
TCGGTAA652.0902364E-532.30769327
ATGCCAT552.9794333E-431.8181843
TCTCCAA450.004294993431.11111365
CTACACG450.004294993431.1111134
CCGCAGT450.004294993431.1111131
CAGTACT703.468073E-530.04
GGTAAGC604.961061E-429.16666629
CGTTCGG604.961061E-429.16666624
TGTATAT755.5517718E-528.03
Produced by FastQC (version 0.11.5)