##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781185_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133751
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30365380445754	34.0	34.0	34.0	31.0	34.0
2	32.43948082631158	34.0	34.0	34.0	31.0	34.0
3	32.46140215774088	34.0	34.0	34.0	31.0	34.0
4	32.563113546814606	34.0	34.0	34.0	31.0	34.0
5	32.50490837451682	34.0	34.0	34.0	31.0	34.0
6	35.68341918938924	38.0	37.0	38.0	31.0	38.0
7	35.66143056874341	38.0	37.0	38.0	31.0	38.0
8	35.513626066347165	38.0	37.0	38.0	31.0	38.0
9	35.738910363287005	38.0	37.0	38.0	31.0	38.0
10-11	35.660021233486106	38.0	37.0	38.0	31.0	38.0
12-13	35.619520601715124	38.0	37.0	38.0	31.0	38.0
14-15	35.542481925368776	38.0	36.5	38.0	31.0	38.0
16-17	35.52103161845519	38.0	36.5	38.0	31.0	38.0
18-19	35.53558478067454	38.0	37.0	38.0	31.0	38.0
20-21	35.49998504684077	38.0	36.5	38.0	31.0	38.0
22-23	35.53133808345358	38.0	36.5	38.0	31.0	38.0
24-25	35.53500534575443	38.0	36.5	38.0	31.0	38.0
26-27	35.46938340647921	38.0	36.0	38.0	31.0	38.0
28-29	35.54763328872308	38.0	37.0	38.0	31.0	38.0
30-31	35.58597692727531	38.0	37.0	38.0	31.0	38.0
32-33	35.551008964418955	38.0	37.0	38.0	31.0	38.0
34-35	35.563278031566114	38.0	37.0	38.0	31.0	38.0
36-37	35.476194570507886	38.0	36.5	38.0	31.0	38.0
38-39	35.46407129666321	38.0	36.0	38.0	31.0	38.0
40-41	35.46316289224006	38.0	36.0	38.0	31.0	38.0
42-43	35.54677348206742	38.0	37.0	38.0	31.0	38.0
44-45	35.5592631083132	38.0	37.0	38.0	31.0	38.0
46-47	35.540545491248665	38.0	36.5	38.0	31.0	38.0
48-49	35.50176447278899	38.0	36.5	38.0	31.0	38.0
50-51	35.4141838191864	38.0	36.0	38.0	31.0	38.0
52-53	35.47545812741587	38.0	36.0	38.0	31.0	38.0
54-55	35.42947716278756	38.0	36.0	38.0	31.0	38.0
56-57	35.39500265418576	38.0	36.0	38.0	30.5	38.0
58-59	35.402853062780835	38.0	36.0	38.0	31.0	38.0
60-61	35.386064403256796	38.0	36.0	38.0	31.0	38.0
62-63	35.34723104873982	38.0	36.0	38.0	29.5	38.0
64-65	35.35607958071341	38.0	36.0	38.0	30.0	38.0
66-67	35.25637565326615	38.0	36.0	38.0	27.5	38.0
68-69	34.98266928845392	38.0	36.0	38.0	27.0	38.0
70-71	35.12947940576145	38.0	36.0	38.0	27.0	38.0
72-73	35.14190174279071	38.0	36.0	38.0	27.0	38.0
74-75	35.16876135505529	38.0	36.0	38.0	27.0	38.0
76	33.90480071177038	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	10.0
14	60.0
15	166.0
16	303.0
17	433.0
18	580.0
19	613.0
20	574.0
21	537.0
22	576.0
23	651.0
24	758.0
25	908.0
26	1044.0
27	1388.0
28	1549.0
29	1997.0
30	2427.0
31	3190.0
32	4142.0
33	5534.0
34	8008.0
35	12575.0
36	22783.0
37	62943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57649662432431	19.171445447136843	9.846655352109517	27.40540257642933
2	22.543382853212314	19.128081285373565	32.4020007327048	25.926535128709315
3	19.169202473252536	21.671613670178168	27.97137965323624	31.18780420333306
4	13.715785302539793	15.882498074780749	35.745527136245705	34.656189486433746
5	13.77559793945466	33.83077509700862	35.35225904853048	17.04136791500624
6	30.24126922415533	36.463278779224076	17.879492489775778	15.415959506844809
7	29.510807395832554	28.271938153733426	21.91684548152911	20.300408968904907
8	25.845040410912816	33.51526343728271	19.470508631711166	21.169187520093306
9	23.909353948755523	15.28736233747785	21.07273964306809	39.730544070698535
10-11	25.22186750005607	23.942998557020132	27.156058646290493	23.679075296633297
12-13	24.85215063812607	22.731792659494136	26.323915335212444	26.092141367167347
14-15	23.193097621700023	23.624122436467765	23.30449865795396	29.87828128387825
16-17	23.72169180043514	26.344102100171213	24.881683127602784	25.052522971790864
18-19	23.110855245942087	26.411765145681155	25.74410658611898	24.733273022257777
20-21	22.774409163295974	26.529521274607294	25.76877929884636	24.927290263250367
22-23	23.215901189523816	25.99681497708428	26.22709362920651	24.56019020418539
24-25	22.99982803866887	26.379989682320133	25.845414239893532	24.774768039117465
26-27	22.083199377948574	26.510829825571395	26.315317268655935	25.09065352782409
28-29	22.852913249246733	26.06672099647853	27.025592332019947	24.054773422254787
30-31	22.954220903021287	26.272700764854097	26.167280992291648	24.605797339832975
32-33	22.543382853212314	26.601670267885847	25.929151931574346	24.925794947327496
34-35	23.444684525723172	25.58560309829459	26.5212970370315	24.44841533895074
36-37	23.431974340378765	25.84653572683569	25.77775119438359	24.943738738401954
38-39	22.575158316573336	26.27157927791194	26.658866101935686	24.494396303579038
40-41	22.972538523076462	25.55532295085644	26.665595023588608	24.806543502478487
42-43	22.770297044508077	25.739246809369646	26.471577782596018	25.018878363526255
44-45	22.29590806797706	25.783358629094362	26.595315175213642	25.325418127714933
46-47	22.47646746566381	25.259624227108585	27.061853743149584	25.202054564078026
48-49	22.014414845496482	25.414389425125794	27.494373873840196	25.076821855537528
50-51	21.98263938213546	25.26336251691576	27.36801967835755	25.385978422591233
52-53	21.87422897772727	24.89476714192791	27.82670783769841	25.40429604264641
54-55	21.371055169680975	25.475323548982807	27.604653423151976	25.54896785818424
56-57	20.980777713811484	25.754199968598368	27.594560040672594	25.670462276917554
58-59	21.493671075356445	25.641303616421556	27.209516190533158	25.65550911768884
60-61	21.020403585767582	25.158316573334034	27.52203721841332	26.299242622485064
62-63	20.797601513259714	25.077569513498965	27.90072597588055	26.224102997360767
64-65	21.505259773758702	25.151587651681105	27.291010908329653	26.052141666230533
66-67	21.372550485603846	24.886916733332836	27.12989061764024	26.610642163423076
68-69	21.339279706319953	24.824861122533665	26.88092051648212	26.95493865466426
70-71	22.23235714125502	24.762431682753775	26.537745512183086	26.46746566380812
72-73	22.498523375526165	24.183744420602462	26.295130503697166	27.022601700174203
74-75	22.56170047326749	24.586358232835643	26.01214196529372	26.839799328603153
76	23.179639778394183	24.113464572227496	25.611023468983408	27.095872180394913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	1.0
12	2.0
13	3.5
14	2.5
15	10.0
16	20.5
17	21.0
18	29.0
19	54.5
20	86.5
21	101.0
22	125.0
23	196.5
24	309.0
25	374.0
26	466.5
27	608.5
28	821.0
29	984.0
30	1132.0
31	1445.5
32	1966.5
33	2322.0
34	2523.5
35	3080.0
36	3867.5
37	4300.0
38	4755.5
39	5812.0
40	6938.5
41	7597.0
42	7730.0
43	7844.0
44	8034.5
45	8006.0
46	7901.0
47	7790.5
48	7244.5
49	6540.5
50	6272.0
51	5753.5
52	4674.0
53	3845.5
54	3578.0
55	3309.5
56	2756.0
57	2233.0
58	1995.0
59	1865.0
60	1636.5
61	1496.0
62	1454.0
63	1439.0
64	1423.5
65	1435.0
66	1510.0
67	1573.0
68	1569.0
69	1552.5
70	1480.0
71	1420.0
72	1409.0
73	1343.5
74	1213.0
75	1137.0
76	1066.5
77	902.0
78	686.5
79	565.0
80	540.5
81	429.0
82	299.0
83	256.0
84	222.5
85	148.5
86	83.5
87	59.0
88	46.0
89	28.0
90	19.5
91	12.0
92	8.0
93	6.0
94	2.0
95	0.5
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	133751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.35625901862416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55766718137546	35.5302016433522
2	14.743437462881579	14.848487114115036
3	5.202518113790236	7.859380490613155
4	2.4958427366670626	5.0272521326943345
5	1.5055232212851881	3.790625864479518
6	1.018529516569664	3.0773601692697627
7	0.7587005582610762	2.674372528055865
8	0.5582610761373085	2.2489551479988936
9	0.49144791542938593	2.227273067117255
>10	2.63689274260601	21.76058496758903
>50	0.031179474997030526	0.9555068747149554
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.476579614358024E-4	0.0
2	0.0	0.0	0.0	7.476579614358024E-4	0.0
3	0.0	0.0	0.0	7.476579614358024E-4	0.0
4	0.0	0.0	0.0	7.476579614358024E-4	0.0
5	0.0	0.0	0.0	7.476579614358024E-4	0.0
6	0.0	0.0	0.0	7.476579614358024E-4	0.0
7	0.0	0.0	0.0	7.476579614358024E-4	0.0
8	0.0	0.0	0.0	7.476579614358024E-4	0.0
9	0.0	0.0	0.0	7.476579614358024E-4	0.0
10	0.0	0.0	0.0	7.476579614358024E-4	0.0
11	0.0	0.0	0.0	7.476579614358024E-4	0.0
12	0.0	0.0	0.0	7.476579614358024E-4	0.0
13	0.0	0.0	0.0	7.476579614358024E-4	0.0
14	0.0	0.0	0.0	7.476579614358024E-4	0.0
15	0.0	0.0	0.0	7.476579614358024E-4	0.0
16	0.0	0.0	0.0	7.476579614358024E-4	0.0
17	0.0	0.0	0.0	7.476579614358024E-4	0.0
18	0.0	0.0	0.0	0.0014953159228716048	0.0
19	0.0	0.0	0.0	0.0014953159228716048	0.0
20	0.0	0.0	0.0	0.0014953159228716048	0.0
21	0.0	0.0	0.0	0.002242973884307407	0.0
22	0.0	0.0	0.0	0.0037382898071790117	0.0
23	0.0	0.0	0.0	0.004485947768614814	0.0
24	0.0	0.0	0.0	0.004485947768614814	0.0
25	0.0	0.0	0.0	0.004485947768614814	0.0
26	0.0	0.0	0.0	0.005233605730050617	0.0
27	0.0	0.0	0.0	0.00971955349866543	0.0
28	0.0	0.0	0.0	0.02317739680450987	0.0
29	0.0	0.0	0.0	0.0321492923417395	0.0
30	0.0	0.0	0.0	0.04485947768614814	0.0
31	0.0	0.0	0.0	0.05756966303055678	0.0
32	0.0	0.0	0.0	0.07401813818214444	0.0
33	0.0	0.0	0.0	0.0957002190637827	0.0
34	0.0	0.0	0.0	0.1300924852898296	0.0
35	0.0	0.0	0.0	0.17644727889884934	0.0
36	0.0	0.0	0.0	0.2639232603868382	0.0
37	0.0	0.0	0.0	0.4194361163654851	0.0
38	0.0	0.0	0.0	0.6085935806087431	0.0
39	0.0	0.0	0.0	0.8613019715740443	0.0
40	0.0	0.0	0.0	1.1566268663411863	0.0
41	0.0	0.0	0.0	1.452699419069764	0.0
42	0.0	0.0	0.0	1.801108029098848	0.0
43	0.0	0.0	0.0	2.2452168581917142	0.0
44	0.0	0.0	0.0	2.757362561775239	0.0
45	0.0	0.0	0.0	3.359974878692496	0.0
46	0.0	0.0	0.0	4.0238951484474885	0.0
47	0.0	0.0	0.0	4.725198316274271	0.0
48	0.0	0.0	0.0	5.393604533797878	0.0
49	0.0	0.0	0.0	6.085935806087432	0.0
50	0.0	0.0	0.0	6.678828569506022	0.0
51	0.0	0.0	0.0	7.229852487084209	0.0
52	0.0	0.0	0.0	7.798820195736854	0.0
53	0.0	0.0	0.0	8.38573169546396	0.0
54	0.0	0.0	0.0	9.00703546141711	0.0
55	0.0	0.0	0.0	9.65525491398195	0.0
56	0.0	0.0	0.0	10.33936194869571	0.0
57	0.0	0.0	0.0	10.997300954759217	0.0
58	0.0	0.0	0.0	11.67542672578149	0.0
59	0.0	0.0	0.0	12.274300752891568	0.0
60	0.0	0.0	0.0	12.89186622903754	0.0
61	0.0	0.0	0.0	13.504945757414898	0.0
62	0.0	0.0	0.0	14.129987813175228	0.0
63	0.0	0.0	0.0	14.706432101442232	0.0
64	0.0	0.0	0.0	15.359885159737123	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCATAG	15	0.0022205971	70.0	3
CGTTGGT	20	0.00693868	52.500004	14
TTAGCCA	30	5.860687E-4	46.666664	4
GTAATTG	30	5.860687E-4	46.666664	8
CGATTAG	45	9.1583584E-5	38.88889	52
TTGCGAT	45	9.1583584E-5	38.88889	49
TTCGGTA	45	9.1583584E-5	38.88889	26
GTAAGCA	45	9.1583584E-5	38.88889	30
CGGTAAG	45	9.1583584E-5	38.88889	28
TCGTTCG	50	1.7024306E-4	35.0	23
GTTCGGT	50	1.7024306E-4	35.0	25
TCGGTAA	65	2.0902364E-5	32.307693	27
ATGCCAT	55	2.9794333E-4	31.81818	43
TCTCCAA	45	0.0042949934	31.111113	65
CTACACG	45	0.0042949934	31.111113	4
CCGCAGT	45	0.0042949934	31.111113	1
CAGTACT	70	3.468073E-5	30.0	4
GGTAAGC	60	4.961061E-4	29.166666	29
CGTTCGG	60	4.961061E-4	29.166666	24
TGTATAT	75	5.5517718E-5	28.0	3
>>END_MODULE