FastQCFastQC Report
Wed 25 May 2016
SRR1781185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126087
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT11050.8763790081451697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG150.002220299370.057
GCACGAC150.002220299370.011
CGTAACC150.002220299370.064
TTCGTAA252.3828511E-455.99999620
CGGAACA200.006937757652.562
TCCGCTA200.006937757652.514
CATATCG200.006937757652.558
TCGGGGA200.006937757652.555
TTTCCAG200.006937757652.535
TACGGAG200.006937757652.525
CTACGGA200.006937757652.524
GCAGCGT407.4308264E-752.51
GAACAAT200.006937757652.539
ACAAGTT200.006937757652.566
GTACAGA352.0768137E-550.01
AGCGTCA506.142727E-848.9999963
CACGACA305.8593944E-446.66666412
ATGAACG305.8593944E-446.66666469
CGTAAGG305.8593944E-446.66666422
TCAGATG551.3001954E-744.5454527
Produced by FastQC (version 0.11.5)