FastQCFastQC Report
Wed 25 May 2016
SRR1781184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95166
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTT150.002218620970.065
GCTGCGT150.002218620970.061
CTAAGCG150.002218620970.040
GCGTTTT150.002218620970.044
AGCGTTT200.00693255452.543
CGTGGGG200.00693255452.522
ACATTCA200.00693255452.516
ATCACGG200.00693255452.516
CCGAATG200.00693255452.59
TTGCCGA200.00693255452.516
GCGTGCT200.00693255452.564
CGTTTTG200.00693255452.545
CGTTTGG200.00693255452.528
ATTCGCC305.852107E-446.66666425
AGCCCGA400.002408335735.06
TCCTGCG400.002408335735.069
TTGTATA400.002408335735.02
ACACTAT400.002408335735.06
TTCGCCT400.002408335735.026
TAACACT652.0839321E-532.3076934
Produced by FastQC (version 0.11.5)