##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781184_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95166
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33214593447239	34.0	34.0	34.0	31.0	34.0
2	32.36859802870773	34.0	34.0	34.0	31.0	34.0
3	32.326335035621966	34.0	34.0	34.0	31.0	34.0
4	32.45408023874073	34.0	34.0	34.0	31.0	34.0
5	32.492066494336214	34.0	34.0	34.0	31.0	34.0
6	35.56241724985814	38.0	37.0	38.0	31.0	38.0
7	35.500168127272346	38.0	37.0	38.0	31.0	38.0
8	35.34468192421663	38.0	36.0	38.0	30.0	38.0
9	35.67626042914486	38.0	37.0	38.0	31.0	38.0
10-11	35.58627030662211	38.0	37.0	38.0	31.0	38.0
12-13	35.53804404934536	38.0	37.0	38.0	31.0	38.0
14-15	35.49078977786184	38.0	37.0	38.0	31.0	38.0
16-17	35.47308912847025	38.0	36.5	38.0	31.0	38.0
18-19	35.49546581762394	38.0	37.0	38.0	31.0	38.0
20-21	35.447449719437614	38.0	36.5	38.0	31.0	38.0
22-23	35.4877267091188	38.0	37.0	38.0	31.0	38.0
24-25	35.48978626820503	38.0	37.0	38.0	31.0	38.0
26-27	35.419608893932704	38.0	37.0	38.0	31.0	38.0
28-29	35.5154887249648	38.0	37.0	38.0	31.0	38.0
30-31	35.60240001681272	38.0	37.0	38.0	31.0	38.0
32-33	35.53781287434588	38.0	37.0	38.0	31.0	38.0
34-35	35.528534350503335	38.0	37.0	38.0	31.0	38.0
36-37	35.448290355799344	38.0	37.0	38.0	31.0	38.0
38-39	35.47479141710275	38.0	37.0	38.0	31.0	38.0
40-41	35.4647090347393	38.0	36.5	38.0	31.0	38.0
42-43	35.54359750331001	38.0	37.0	38.0	31.0	38.0
44-45	35.54560452262362	38.0	37.0	38.0	31.0	38.0
46-47	35.515215518147244	38.0	37.0	38.0	31.0	38.0
48-49	35.48794737616376	38.0	37.0	38.0	31.0	38.0
50-51	35.36867683836664	38.0	36.5	38.0	30.5	38.0
52-53	35.44971418363701	38.0	36.5	38.0	31.0	38.0
54-55	35.42384359960491	38.0	36.0	38.0	31.0	38.0
56-57	35.35615661055419	38.0	36.0	38.0	29.5	38.0
58-59	35.351039236702185	38.0	36.0	38.0	30.0	38.0
60-61	35.35174326965513	38.0	36.0	38.0	29.5	38.0
62-63	35.336716894689275	38.0	36.0	38.0	29.0	38.0
64-65	35.3528728747662	38.0	36.0	38.0	30.0	38.0
66-67	35.24941680852405	38.0	36.0	38.0	27.0	38.0
68-69	34.96081058361179	38.0	36.0	38.0	27.0	38.0
70-71	35.169115020070194	38.0	36.0	38.0	27.0	38.0
72-73	35.18958451547822	38.0	36.0	38.0	27.0	38.0
74-75	35.185418111510415	38.0	36.0	38.0	27.0	38.0
76	34.04703360443856	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	14.0
14	49.0
15	122.0
16	281.0
17	384.0
18	489.0
19	486.0
20	454.0
21	423.0
22	425.0
23	454.0
24	559.0
25	625.0
26	760.0
27	921.0
28	1078.0
29	1336.0
30	1753.0
31	2243.0
32	2784.0
33	3912.0
34	5537.0
35	8435.0
36	15827.0
37	45813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96914864552466	19.097156547506465	9.125107706533846	25.808587100435034
2	22.769686652796167	19.648824159889035	32.613538448605595	24.967950738709202
3	19.157051888279426	22.23378097219595	29.51053947838514	29.09862766113948
4	13.293613265241788	16.276821553916314	37.67627093709938	32.753294243742516
5	13.25368303805981	34.259084126683895	36.58239287140365	15.904839963852638
6	29.770085955067987	37.93476661833007	17.960195868272283	14.334951558329657
7	28.439778912636864	29.60300947817498	22.847445516255803	19.10976609293235
8	25.33362755605994	34.41565264905533	19.717125864279257	20.533593930605466
9	24.519261080638046	14.790996784565916	21.210306201794758	39.479435933001284
10-11	24.64771712289182	24.63248042872905	28.428518888246728	22.291283560132403
12-13	24.634848580375344	23.070739549839228	27.075846415736716	25.218565454048715
14-15	23.048672845343926	24.126788979257295	24.465670512578022	28.358867662820753
16-17	23.069163356660994	27.22295777903873	25.751844146018538	23.95603471828174
18-19	22.50646239203077	27.127860790618495	26.835739654918772	23.52993716243196
20-21	22.499632221591746	27.561839312359453	26.579345564592394	23.359182901456403
22-23	22.878969379820525	26.515772439736878	26.98915579093374	23.616102389508857
24-25	22.472311539835655	26.9802240295904	26.85307777987937	23.694386650694575
26-27	21.78561671185087	27.004917722716097	27.32751192652838	23.88195363890465
28-29	22.40453523317151	27.15518147237459	27.705798289305005	22.734485005148898
30-31	22.700859550679866	27.096336927053784	27.05325431351533	23.149549208751026
32-33	22.027825063573125	27.309123006115634	27.147300506483408	23.515751423827837
34-35	23.005590231805474	26.761133177815605	27.212449824517158	23.020826765861756
36-37	22.978269550049387	26.687052098438517	26.674967950738708	23.659710400773385
38-39	22.317844608368535	27.22085618813442	27.348002437845448	23.113296765651597
40-41	22.53693546014333	26.301935565222873	27.845554084441922	23.315574890191876
42-43	22.26530483576067	26.80579198453229	27.419456528592146	23.509446651114892
44-45	22.025198074942733	26.650799655339092	27.421032721770377	23.902969547947798
46-47	21.96057415463506	26.440640564907635	27.698442721139905	23.9003425593174
48-49	21.63114978038375	26.637139314461045	28.104049765672613	23.62766113948259
50-51	21.942185234222304	26.449046928524893	27.786709539121112	23.822058298131683
52-53	21.991572620473697	26.063404997583174	28.117184708824578	23.82783767311855
54-55	21.234999894920456	26.571464598701215	28.2422293676313	23.95130613874703
56-57	21.285963474350083	26.642918689447914	28.03574806128239	24.035369774919616
58-59	21.397873190004834	26.32295147426602	27.893890675241156	24.385284660487986
60-61	21.232372906290063	26.165332156442428	28.008952777252382	24.59334216001513
62-63	20.737448248323982	26.07076056574827	28.722442889267175	24.469348296660574
64-65	21.57230523506294	26.208414769980877	27.926990731984112	24.29228926297207
66-67	21.365298530987957	25.88214278208604	27.821385789042306	24.931172897883698
68-69	21.04638211125822	26.412269087699386	27.46411533530883	25.077233465733563
70-71	22.015215518147237	25.886871361620745	27.393186642288214	24.704726477943804
72-73	21.82817392766324	25.411386419519577	27.32593573335015	25.434503919467037
74-75	21.819767564045982	25.872160225290546	27.10106550658849	25.207006704074985
76	22.517495744278417	24.980560284135088	27.06744005211946	25.434503919467037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	2.0
11	3.0
12	3.0
13	2.5
14	5.5
15	15.5
16	27.5
17	33.0
18	49.0
19	71.5
20	96.0
21	114.0
22	139.0
23	214.0
24	317.0
25	370.0
26	437.5
27	566.5
28	753.0
29	878.0
30	1015.0
31	1244.0
32	1597.5
33	1859.0
34	2087.0
35	2512.5
36	3046.0
37	3382.0
38	3614.5
39	4296.5
40	5118.0
41	5526.0
42	5562.0
43	5682.0
44	5796.5
45	5752.5
46	5714.0
47	5689.5
48	5265.5
49	4722.5
50	4579.0
51	4274.0
52	3549.5
53	2915.0
54	2700.0
55	2553.5
56	2268.5
57	1945.5
58	1761.0
59	1543.0
60	1197.5
61	1004.0
62	938.0
63	866.5
64	754.0
65	728.5
66	707.0
67	670.0
68	666.0
69	648.5
70	591.5
71	548.0
72	566.0
73	532.0
74	459.0
75	438.0
76	399.0
77	331.5
78	264.5
79	226.0
80	215.5
81	175.5
82	117.0
83	88.0
84	77.0
85	54.5
86	34.0
87	25.0
88	19.5
89	13.5
90	11.0
91	6.5
92	4.0
93	4.5
94	2.5
95	0.5
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.001050795452157283
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	95166.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.022466006767125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05495153503114	41.65773490532333
2	14.605830538458703	16.65720950759725
3	4.724873769948034	8.082718617993821
4	2.1800022113293775	4.972364079608264
5	1.2088600597058932	3.4466090830758884
6	0.895588397891866	3.0641195384906372
7	0.665241587734493	2.6553601076014544
8	0.4883352375336306	2.22768635857344
9	0.4109387093207534	2.1089464724796674
>10	1.7635351785648474	15.063152806674655
>50	0.0018427744812589838	0.06409852258159428
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0010507954521572832	0.0
10	0.0	0.0	0.0	0.0010507954521572832	0.0
11	0.0	0.0	0.0	0.0010507954521572832	0.0
12	0.0	0.0	0.0	0.0010507954521572832	0.0
13	0.0	0.0	0.0	0.0010507954521572832	0.0
14	0.0	0.0	0.0	0.0010507954521572832	0.0
15	0.0	0.0	0.0	0.0010507954521572832	0.0
16	0.0	0.0	0.0	0.0010507954521572832	0.0
17	0.0	0.0	0.0	0.0010507954521572832	0.0
18	0.0	0.0	0.0	0.0010507954521572832	0.0
19	0.0	0.0	0.0	0.0021015909043145663	0.0
20	0.0	0.0	0.0	0.0021015909043145663	0.0
21	0.0	0.0	0.0	0.0021015909043145663	0.0
22	0.0	0.0	0.0	0.007355568165100981	0.0
23	0.0	0.0	0.0	0.007355568165100981	0.0
24	0.0	0.0	0.0	0.009457159069415548	0.0
25	0.0	0.0021015909043145663	0.0	0.009457159069415548	0.0
26	0.0	0.0021015909043145663	0.0	0.009457159069415548	0.0
27	0.0	0.0021015909043145663	0.0	0.012609545425887396	0.0
28	0.0	0.0021015909043145663	0.0	0.01786352268667381	0.0
29	0.0	0.0021015909043145663	0.0	0.024168295399617512	0.0
30	0.0	0.0021015909043145663	0.0	0.02837147720824664	0.0
31	0.0	0.0021015909043145663	0.0	0.03572704537334762	0.0
32	0.0	0.0021015909043145663	0.0	0.04413340899060589	0.0
33	0.0	0.0021015909043145663	0.0	0.0683017043902234	0.0
34	0.0	0.0021015909043145663	0.0	0.09667318159847005	0.0
35	0.0	0.0021015909043145663	0.0	0.13765420423260408	0.0
36	0.0	0.0021015909043145663	0.0	0.20805749952714206	0.0
37	0.0	0.0021015909043145663	0.0	0.30683227202992663	0.0
38	0.0	0.0021015909043145663	0.0	0.44028329445390163	0.0
39	0.0	0.0021015909043145663	0.0	0.5632263623563037	0.0
40	0.0	0.0021015909043145663	0.0	0.760775907361873	0.0
41	0.0	0.0021015909043145663	0.0	0.9488682932980266	0.0
42	0.0	0.0021015909043145663	0.0	1.2000084063636172	0.0
43	0.0	0.0021015909043145663	0.0	1.4784692011852973	0.0
44	0.0	0.0021015909043145663	0.0	1.7958094277367969	0.0
45	0.0	0.0021015909043145663	0.0	2.212975222243238	0.0
46	0.0	0.0021015909043145663	0.0	2.6816299939053865	0.0
47	0.0	0.0021015909043145663	0.0	3.1734022655149947	0.0
48	0.0	0.0021015909043145663	0.0	3.663072946220289	0.0
49	0.0	0.0021015909043145663	0.0	4.064476808944371	0.0
50	0.0	0.0021015909043145663	0.0	4.469033058024925	0.0
51	0.0	0.0021015909043145663	0.0	4.903011579765883	0.0
52	0.0	0.0021015909043145663	0.0	5.297059874324864	0.0
53	0.0	0.0021015909043145663	0.0	5.662736691675598	0.0
54	0.0	0.0021015909043145663	0.0	6.120883508816174	0.0
55	0.0	0.0021015909043145663	0.0	6.62526532585167	0.0
56	0.0	0.0021015909043145663	0.0	7.085513733896559	0.0
57	0.0	0.0021015909043145663	0.0	7.604606687262257	0.0
58	0.0	0.0021015909043145663	0.0	8.094277367967551	0.0
59	0.0	0.0021015909043145663	0.0	8.606014753168148	0.0
60	0.0	0.0021015909043145663	0.0	9.13876804741189	0.0
61	0.0	0.0021015909043145663	0.0	9.569594182796378	0.0
62	0.0	0.0021015909043145663	0.0	10.056112477145199	0.0
63	0.0	0.0021015909043145663	0.0	10.515310089737932	0.0
64	0.0	0.0021015909043145663	0.0	10.977660088687136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTT	15	0.0022186209	70.0	65
GCTGCGT	15	0.0022186209	70.0	61
CTAAGCG	15	0.0022186209	70.0	40
GCGTTTT	15	0.0022186209	70.0	44
AGCGTTT	20	0.006932554	52.5	43
CGTGGGG	20	0.006932554	52.5	22
ACATTCA	20	0.006932554	52.5	16
ATCACGG	20	0.006932554	52.5	16
CCGAATG	20	0.006932554	52.5	9
TTGCCGA	20	0.006932554	52.5	16
GCGTGCT	20	0.006932554	52.5	64
CGTTTTG	20	0.006932554	52.5	45
CGTTTGG	20	0.006932554	52.5	28
ATTCGCC	30	5.852107E-4	46.666664	25
AGCCCGA	40	0.0024083357	35.0	6
TCCTGCG	40	0.0024083357	35.0	69
TTGTATA	40	0.0024083357	35.0	2
ACACTAT	40	0.0024083357	35.0	6
TTCGCCT	40	0.0024083357	35.0	26
TAACACT	65	2.0839321E-5	32.307693	4
>>END_MODULE