FastQCFastQC Report
Wed 25 May 2016
SRR1781184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89864
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT8940.9948366420368556No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG990.11016647378260483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT150.00221821770.0000141
GTAAGGT150.00221821770.0000121
TATGTCG150.00221821770.0000143
CCTCGTA252.379153E-455.99999638
CTCGTAT1350.054.44444339
TCTCGTA1050.053.33333238
CTAGAAC200.00693130352.524
CGAAATA200.00693130352.556
GCGAAAT200.00693130352.555
GTCTAAC200.00693130352.51
CTAAAGT200.00693130352.516
CAAGGTT200.00693130352.56
GATTGGC200.00693130352.541
GGCGAAA200.00693130352.554
CGATAGC200.00693130352.550
TCGTATG1500.051.33333240
CGTATGC1250.050.441
TATGCCG1250.050.443
ATCTCGT1050.049.99999637
CATCTCG1100.047.72727236
Produced by FastQC (version 0.11.5)