##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781184_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89864
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.602443692691175	34.0	34.0	34.0	31.0	34.0
2	32.85479168521321	34.0	34.0	34.0	31.0	34.0
3	32.96660509213923	34.0	34.0	34.0	31.0	34.0
4	33.11705466037568	34.0	34.0	34.0	31.0	34.0
5	33.053859164960386	34.0	34.0	34.0	31.0	34.0
6	36.1910331167097	38.0	37.0	38.0	34.0	38.0
7	36.079297605270185	38.0	36.0	38.0	32.0	38.0
8	35.71558132288792	38.0	36.0	38.0	31.0	38.0
9	35.88058621917564	38.0	36.0	38.0	31.0	38.0
10-11	35.75948655746461	38.0	36.0	38.0	31.0	38.0
12-13	35.68591983441645	38.0	36.0	38.0	31.0	38.0
14-15	35.73273502181074	38.0	36.0	38.0	31.0	38.0
16-17	35.64213144306953	38.0	36.0	38.0	31.0	38.0
18-19	35.6310480281314	38.0	36.0	38.0	31.0	38.0
20-21	35.56241097658685	38.0	36.0	38.0	31.0	38.0
22-23	35.61394440487848	38.0	36.0	38.0	31.0	38.0
24-25	35.64723359743613	38.0	36.0	38.0	31.0	38.0
26-27	35.35425754473427	38.0	36.0	38.0	30.5	38.0
28-29	35.30183944627437	38.0	36.0	38.0	31.0	38.0
30-31	35.323588978901455	38.0	36.0	38.0	31.0	38.0
32-33	35.27021944271343	38.0	36.0	38.0	30.5	38.0
34-35	35.273824890946315	38.0	36.0	38.0	29.5	38.0
36-37	35.22409418677112	37.5	35.5	38.0	28.5	38.0
38-39	35.15877882133001	38.0	35.0	38.0	27.0	38.0
40-41	35.200436214724476	37.5	35.0	38.0	28.5	38.0
42-43	35.19298606783584	37.0	35.0	38.0	27.0	38.0
44-45	35.134141591738626	37.0	35.0	38.0	27.0	38.0
46-47	35.181780245704616	37.0	35.0	38.0	28.0	38.0
48-49	35.14548094898959	37.0	35.0	38.0	27.0	38.0
50-51	35.02992855871094	37.0	35.0	38.0	27.0	38.0
52-53	35.047905724205464	37.0	35.0	38.0	27.0	38.0
54-55	35.07396732840738	37.0	35.0	38.0	27.0	38.0
56-57	35.00753360633847	37.0	35.0	38.0	27.0	38.0
58-59	34.98686348259592	37.0	35.0	38.0	27.0	38.0
60-61	34.975958114484115	37.0	35.0	38.0	27.0	38.0
62-63	34.94976853912579	37.0	35.0	38.0	27.0	38.0
64-65	34.89082391168877	37.0	35.0	38.0	27.0	38.0
66-67	34.86669300275973	37.0	35.0	38.0	27.0	38.0
68-69	34.77428113593875	37.0	34.5	38.0	27.0	38.0
70-71	34.71188128727855	37.0	34.5	38.0	27.0	38.0
72-73	34.62705310246595	37.0	34.0	38.0	27.0	38.0
74-75	34.653871405679695	37.0	34.5	38.0	27.0	38.0
76	33.68845143772812	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	11.0
22	26.0
23	67.0
24	156.0
25	282.0
26	497.0
27	825.0
28	1322.0
29	1997.0
30	2966.0
31	4060.0
32	5405.0
33	7234.0
34	9191.0
35	11900.0
36	17199.0
37	26724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49056351820529	20.014688863171017	9.294044333659752	26.200703284963943
2	22.768850707736135	20.58110032938663	32.16861034452061	24.481438618356627
3	19.85889789014511	23.096011751090536	29.733819994658596	27.31127036410576
4	13.915472269206802	17.612169500578652	37.34977299029645	31.1225852399181
5	13.339045669010948	35.54927445918277	36.51406569927891	14.597614172527374
6	30.46492477521588	39.165850618712724	17.206000178046825	13.16322442802457
7	28.673328585417966	30.128861390545715	21.584839312739252	19.61297071129707
8	25.166918899670616	36.27036410575982	18.658194605181162	19.90452238938841
9	24.325647645330722	15.082791774236624	20.596679426689217	39.994881153743435
10-11	24.636116798718064	25.708292530935633	28.067412979613636	21.588177690732664
12-13	24.986646488026352	23.235667230481617	26.995237247396066	24.78244903409597
14-15	22.905167809133804	24.261662067123655	23.74922104513487	29.083949078607674
16-17	23.77704086174664	27.983953529778333	24.85422416095433	23.384781447520698
18-19	23.45321819638565	27.05644084394196	25.99205466037568	23.498286299296716
20-21	22.986958069972403	26.913446986557467	25.80733107807353	24.2922638653966
22-23	22.714323867177065	27.263976675865752	26.478345054749397	23.54335440220778
24-25	23.577294578474138	27.325180272411643	26.18957535831924	22.90794979079498
26-27	22.690955221223184	27.825380575091245	26.634136027775305	22.849528175910265
28-29	23.47324846434612	26.594631888186594	27.09928336152408	22.832836285943202
30-31	23.033695361880174	27.782538057509125	26.846679426689217	22.33708715392148
32-33	22.254740496750646	27.86822309267337	27.12209561114573	22.75494079943025
34-35	23.752559423128282	26.27247841182231	27.077027508234664	22.89793465681474
36-37	23.00754473426511	27.0241698566723	26.064942579898513	23.90334282916407
38-39	22.236935814119114	27.20166028665539	27.411978100240365	23.14942579898513
40-41	22.250845722424998	26.377637318614795	28.105247930205646	23.266269028754564
42-43	23.289637674708448	26.313095344075492	27.68683788836464	22.71042909285142
44-45	22.209115997507347	26.96240986379418	27.567212676934034	23.261261461764445
46-47	22.906280601798272	26.114461853467464	27.203885871984333	23.775371672749934
48-49	22.698744769874477	25.841827650672126	27.6940710406837	23.765356538769698
50-51	22.08114484109321	25.822910175376123	28.07965369892282	24.016291284607853
52-53	21.806285052968928	25.810113059734714	29.191889967061336	23.191711920235022
54-55	21.279377726342027	26.13449212142794	28.69168966438173	23.894440487848303
56-57	21.793487937327516	26.13671770675688	27.955020920502093	24.114773435413515
58-59	21.387318614795692	25.98203952639544	28.976564586486248	23.65407727232262
60-61	21.82297694293599	26.902875456244992	27.503783495059203	23.770364105759814
62-63	20.612814920324045	27.370804771654946	27.928313896554798	24.088066411466215
64-65	20.938863171013978	27.795335173150537	27.105960117510907	24.15984153832458
66-67	21.012307486869048	27.38026350930295	26.998019229057242	24.609409774770764
68-69	20.891013086441735	28.059623430962343	26.449968841805394	24.59939464079053
70-71	21.296626012641326	27.331300632066235	26.67697854535743	24.695094809935014
72-73	20.93830677468174	26.747084483219087	26.896755096590404	25.41785364550877
74-75	21.006187127214456	26.581278376212946	26.872273657972045	25.540260838600553
76	21.65717083592985	26.688106472002136	26.488916585061872	25.165806107006144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	1.0
14	4.0
15	7.0
16	21.0
17	34.0
18	37.0
19	58.5
20	99.5
21	122.0
22	153.0
23	222.5
24	311.5
25	362.0
26	429.0
27	586.0
28	761.5
29	847.0
30	944.0
31	1161.0
32	1514.5
33	1748.0
34	1960.5
35	2366.0
36	2748.5
37	2938.0
38	3275.0
39	3957.5
40	4673.5
41	5226.5
42	5409.0
43	5590.0
44	5781.0
45	5746.5
46	5702.0
47	5569.0
48	5053.5
49	4432.5
50	4194.0
51	3855.0
52	3266.0
53	2783.0
54	2550.0
55	2470.0
56	2175.0
57	1725.5
58	1491.0
59	1321.0
60	1087.5
61	965.0
62	906.0
63	813.5
64	686.5
65	629.0
66	607.0
67	608.0
68	602.0
69	582.0
70	570.0
71	572.0
72	538.0
73	478.0
74	424.0
75	396.0
76	378.5
77	331.0
78	269.5
79	238.0
80	199.0
81	139.0
82	102.0
83	86.0
84	71.5
85	49.0
86	26.0
87	11.0
88	13.0
89	9.5
90	2.5
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	89864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.69456066945606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64465729763681	41.185569304727146
2	15.011384156394755	17.02127659574468
3	4.685169192117454	7.968708270275082
4	2.3396404176807724	5.305795424196564
5	1.2797362016173355	3.6277040861746643
6	0.8773651566302897	2.984509926110567
7	0.6909005260265368	2.7419211252559426
8	0.4691057548873361	2.127659574468085
9	0.382743189134019	1.9529511261461765
>10	1.6075213943628799	13.686236980325825
>50	0.009813927926513307	0.40283094453841356
>100	0.0	0.0
>500	0.0019627855853026617	0.9948366420368556
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	894	0.9948366420368556	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	99	0.11016647378260483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0011127926644707558	0.0
10	0.0	0.0	0.0	0.0011127926644707558	0.0
11	0.0	0.0	0.0	0.0011127926644707558	0.0
12	0.0	0.0	0.0	0.0011127926644707558	0.0
13	0.0	0.0	0.0	0.0011127926644707558	0.0
14	0.0	0.0	0.0	0.0011127926644707558	0.0
15	0.0	0.0	0.0	0.0011127926644707558	0.0
16	0.0	0.0	0.0	0.0011127926644707558	0.0
17	0.0	0.0	0.0	0.0011127926644707558	0.0
18	0.0	0.0	0.0	0.0011127926644707558	0.0
19	0.0	0.0	0.0	0.0022255853289415116	0.0
20	0.0	0.0	0.0	0.0022255853289415116	0.0
21	0.0	0.0	0.0	0.0022255853289415116	0.0
22	0.0	0.0	0.0	0.0022255853289415116	0.0
23	0.0	0.0	0.0	0.0033383779934122674	0.0
24	0.0	0.0	0.0	0.0033383779934122674	0.0
25	0.0	0.0	0.0	0.0033383779934122674	0.0
26	0.0	0.0	0.0	0.0033383779934122674	0.0
27	0.0	0.0	0.0	0.005563963322353779	0.0
28	0.0	0.0	0.0	0.011127926644707558	0.0
29	0.0	0.0	0.0	0.015579097302590581	0.0
30	0.0	0.0	0.0	0.020030267960473604	0.0
31	0.0	0.0	0.0	0.02893260927623965	0.0
32	0.0	0.0	0.0	0.03672215792753494	0.0
33	0.0	0.0	0.0	0.06342918187483308	0.0
34	0.0	0.0	0.0	0.09124899848660198	0.0
35	0.0	0.0	0.0	0.13464791240096144	0.0
36	0.0	0.0	0.0	0.20364105759814832	0.0
37	0.0	0.0	0.0	0.3071307753939286	0.0
38	0.0	0.0	0.0	0.43176355381465326	0.0
39	0.0	0.0	0.0	0.5541707469064364	0.0
40	0.0	0.0	0.0	0.7578118045045847	0.0
41	0.0	0.0	0.0	0.9425353868067302	0.0
42	0.0	0.0	0.0	1.1862369803258257	0.0
43	0.0	0.0	0.0	1.4566455977922192	0.0
44	0.0	0.0	0.0	1.7693403365085016	0.0
45	0.0	0.0	0.0	2.174396866375857	0.0
46	0.0	0.0	0.0	2.6373186147956913	0.0
47	0.0	0.0	0.0	3.116932253182587	0.0
48	0.0	0.0	0.0	3.5987714768984245	0.0
49	0.0	0.0	0.0	3.9904744947921302	0.0
50	0.0	0.0	0.0	4.391079854001602	0.0
51	0.0	0.0	0.0	4.816166651829431	0.0
52	0.0	0.0	0.0	5.198967328407371	0.0
53	0.0	0.0	0.0	5.5617377370248375	0.0
54	0.0	0.0	0.0	6.000178046826315	0.0
55	0.0	0.0	0.0	6.489806819193448	0.0
56	0.0	0.0	0.0	6.9338110923172795	0.0
57	0.0	0.0	0.0	7.476853912579008	0.0
58	0.0	0.0	0.0	7.95090358764355	0.0
59	0.0	0.0	0.0	8.486156859253985	0.0
60	0.0	0.0	0.0	9.006943826226298	0.0
61	0.0	0.0	0.0	9.429805038725185	0.0
62	0.0	0.0	0.0	9.904967506454197	0.0
63	0.0	0.0	0.0	10.372340425531915	0.0
64	0.0	0.0	0.0	10.83526217395175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	15	0.002218217	70.00001	41
GTAAGGT	15	0.002218217	70.00001	21
TATGTCG	15	0.002218217	70.00001	43
CCTCGTA	25	2.379153E-4	55.999996	38
CTCGTAT	135	0.0	54.444443	39
TCTCGTA	105	0.0	53.333332	38
CTAGAAC	20	0.006931303	52.5	24
CGAAATA	20	0.006931303	52.5	56
GCGAAAT	20	0.006931303	52.5	55
GTCTAAC	20	0.006931303	52.5	1
CTAAAGT	20	0.006931303	52.5	16
CAAGGTT	20	0.006931303	52.5	6
GATTGGC	20	0.006931303	52.5	41
GGCGAAA	20	0.006931303	52.5	54
CGATAGC	20	0.006931303	52.5	50
TCGTATG	150	0.0	51.333332	40
CGTATGC	125	0.0	50.4	41
TATGCCG	125	0.0	50.4	43
ATCTCGT	105	0.0	49.999996	37
CATCTCG	110	0.0	47.727272	36
>>END_MODULE