FastQCFastQC Report
Wed 25 May 2016
SRR1781183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215887
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG500.00719760627.99999864
ATCAGGT500.00719760627.99999812
AGGGCCG500.00719760627.99999869
TGCCGCT500.00719760627.99999866
ACGAGGG906.1451665E-627.22222369
CGAGGGC1101.0105105E-625.45454870
GACCCAT700.001223329225.06
GGTCTGG700.001223329225.01
GGTTAGA700.001223329225.01
ATCCATA1052.0306967E-523.3333341
CTAGACT800.002660202321.8750024
CTACACC800.002660202321.8750024
CGACGAG1355.9874E-620.7407469
GTCTGAG850.003780945220.5882361
ATAACAC1055.4468296E-420.0000023
GTAACTT1055.4468296E-420.00000238
CTGCCGA3800.019.34210470
GACGAGG1451.1103057E-519.31034570
GATCTAG950.007192115318.4210511
ACACCAT1602.591555E-517.5000026
Produced by FastQC (version 0.11.5)