##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781183_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	215887
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92407138919898	34.0	34.0	34.0	32.0	34.0
2	33.05747914418191	34.0	34.0	34.0	33.0	34.0
3	33.04298545072191	34.0	34.0	34.0	33.0	34.0
4	33.094447558213325	34.0	34.0	34.0	33.0	34.0
5	33.077619310102044	34.0	34.0	34.0	33.0	34.0
6	36.48058011830263	38.0	37.0	38.0	34.0	38.0
7	36.384603982639064	38.0	37.0	38.0	34.0	38.0
8	36.459416268696124	38.0	37.0	38.0	34.0	38.0
9	36.5596399968502	38.0	37.0	38.0	34.0	38.0
10-11	36.50169764738034	38.0	37.0	38.0	34.0	38.0
12-13	36.45505287488362	38.0	37.0	38.0	34.0	38.0
14-15	36.43406735931298	38.0	37.0	38.0	34.0	38.0
16-17	36.447183017041326	38.0	37.0	38.0	34.0	38.0
18-19	36.4741184045357	38.0	37.0	38.0	34.0	38.0
20-21	36.44929754918083	38.0	37.0	38.0	34.0	38.0
22-23	36.478766669600304	38.0	37.0	38.0	34.0	38.0
24-25	36.4927299930056	38.0	37.0	38.0	34.0	38.0
26-27	36.46652415383974	38.0	37.0	38.0	34.0	38.0
28-29	36.53337857304979	38.0	37.0	38.0	34.0	38.0
30-31	36.58633451759485	38.0	37.0	38.0	34.0	38.0
32-33	36.57352457535656	38.0	37.0	38.0	34.0	38.0
34-35	36.57682028098033	38.0	37.0	38.0	34.0	38.0
36-37	36.52201151528346	38.0	37.0	38.0	34.0	38.0
38-39	36.505113786378985	38.0	37.0	38.0	34.0	38.0
40-41	36.491553451574205	38.0	37.0	38.0	34.0	38.0
42-43	36.56414003622265	38.0	37.0	38.0	34.0	38.0
44-45	36.55469528040132	38.0	37.0	38.0	34.0	38.0
46-47	36.52759082297683	38.0	37.0	38.0	34.0	38.0
48-49	36.50240866749735	38.0	37.0	38.0	34.0	38.0
50-51	36.44803763079759	38.0	37.0	38.0	34.0	38.0
52-53	36.48012617712044	38.0	37.0	38.0	34.0	38.0
54-55	36.46563016763399	38.0	37.0	38.0	34.0	38.0
56-57	36.40090880877496	38.0	37.0	38.0	34.0	38.0
58-59	36.41570821772501	38.0	37.0	38.0	34.0	38.0
60-61	36.40313682621001	38.0	37.0	38.0	34.0	38.0
62-63	36.38359882716421	38.0	37.0	38.0	34.0	38.0
64-65	36.39237656737089	38.0	37.0	38.0	34.0	38.0
66-67	36.31647806491359	38.0	37.0	38.0	34.0	38.0
68-69	36.06570103804305	38.0	37.0	38.0	33.0	38.0
70-71	36.25476754042624	38.0	37.0	38.0	34.0	38.0
72-73	36.26636851686299	38.0	37.0	38.0	34.0	38.0
74-75	36.29117084400636	38.0	37.0	38.0	34.0	38.0
76	35.38393696702442	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	33.0
15	81.0
16	143.0
17	249.0
18	367.0
19	402.0
20	432.0
21	444.0
22	488.0
23	600.0
24	750.0
25	902.0
26	1019.0
27	1343.0
28	1628.0
29	1977.0
30	2494.0
31	3090.0
32	4111.0
33	5624.0
34	8169.0
35	13282.0
36	28956.0
37	139299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41194235873397	21.827159578853752	11.056247018115958	25.704651044296323
2	19.245253303811715	22.371888997484795	35.54174174452376	22.841115954179735
3	18.47725893638802	23.495161820767347	31.279789890081382	26.747789352763252
4	12.712205922542813	16.91208826839967	38.30800372417051	32.067702084887
5	13.245355208975065	34.6088462945893	36.26665802016796	15.879140476267677
6	28.521402400329805	38.40898247694396	18.833463802822774	14.236151319903467
7	25.74911875193967	31.195949732962152	23.84858745547439	19.206344059623785
8	22.218104841884877	36.575152741943704	22.34733911722336	18.85940329894806
9	22.484911087744976	16.356705128145745	23.698045736890133	37.460338047219146
10-11	22.537948093215434	26.298711826094205	29.61804091955514	21.545299161135222
12-13	22.726472645411718	24.372009430859663	29.498997160551582	23.40252076317703
14-15	20.548944586751404	26.420303214181494	26.485383557138686	26.54536864192842
16-17	20.141323933354023	30.23132472080301	27.458346264480955	22.16900508136201
18-19	19.47963517951521	29.68937453389968	29.260446437256526	21.570543849328583
20-21	20.427816403952068	29.355171918642625	28.562396068313518	21.654615609091792
22-23	20.061652623826355	29.001283078647628	28.721275482080905	22.215788815445116
24-25	20.324058419450918	29.052467262966275	28.52533964527739	22.09813467230542
26-27	19.60516381255008	29.01031558176268	29.222926808932453	22.161593796754783
28-29	19.421734518521262	29.051540852390374	29.713692811517134	21.81303181757123
30-31	20.182317601337736	29.502934405499172	28.86371110812601	21.45103688503708
32-33	19.692941214616905	29.21111507408969	29.124264082598767	21.97167962869464
34-35	19.65333716249705	28.7347084354315	29.63263188612561	21.979322515945842
36-37	20.017184916183	29.606460787356347	28.997809038987988	21.37854525747266
38-39	19.82078587409154	29.491817478588334	29.35308749484684	21.334309152473285
40-41	19.72397596890966	29.14279229411683	29.488111836284723	21.645119900688787
42-43	19.779329000819875	28.840319241084455	29.668298693297885	21.712053064797786
44-45	20.040808385868534	28.80974769207965	29.175448266917414	21.9739956551344
46-47	19.863632363227058	29.24377104689027	29.27063695359146	21.62195963629121
48-49	19.503490251844717	28.78357659331039	29.90684941659294	21.806083738251957
50-51	19.82634433754696	28.840319241084455	29.51683056413772	21.816505857230865
52-53	19.4921417222899	28.50820104962318	29.964286872298935	22.035370355787983
54-55	19.704521346815696	28.741424912106794	29.63378989934549	21.92026384173202
56-57	19.268413568209294	28.834297572341082	29.857518053426098	22.03977080602352
58-59	19.26146548889002	28.30415912028052	30.128029941589812	22.306345449239647
60-61	19.27651966074845	28.688619509280315	29.679647222852694	22.355213607118536
62-63	19.179246550278616	28.43478301148286	29.924451217535097	22.461519220703423
64-65	19.274435236952662	28.214760499705864	29.84593792122731	22.664866342114163
66-67	19.143811345750322	28.434319806194907	29.713924414161113	22.70794443389366
68-69	19.32631422920324	27.912982254605417	29.677099593768965	23.083603922422377
70-71	19.80804772867287	28.22309819488899	29.258593616104722	22.710260460333416
72-73	19.65264235456512	27.862956083506653	29.675709977905107	22.808691584023123
74-75	19.88216057474512	27.818951581151254	29.162246916210794	23.13664092789283
76	20.012321260659512	28.053101854210766	28.74513055441041	23.189446330719313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	2.0
9	1.5
10	8.5
11	16.0
12	16.0
13	24.5
14	45.0
15	84.0
16	179.0
17	247.0
18	332.5
19	530.0
20	784.0
21	926.0
22	1162.0
23	1676.5
24	2313.5
25	2672.0
26	3043.5
27	3871.5
28	4757.5
29	5187.0
30	5598.5
31	6529.0
32	7456.5
33	7865.0
34	8297.0
35	9104.5
36	9842.0
37	10204.0
38	10383.5
39	10794.5
40	11074.0
41	11000.5
42	10879.0
43	10718.5
44	10312.0
45	9893.0
46	9720.0
47	9351.5
48	8472.0
49	7657.5
50	7354.0
51	6871.5
52	6032.0
53	5517.0
54	5359.0
55	5198.0
56	4861.5
57	4412.0
58	4138.0
59	3796.5
60	3091.0
61	2425.0
62	2123.0
63	1855.5
64	1420.5
65	1104.5
66	859.5
67	763.0
68	670.0
69	517.5
70	407.5
71	357.0
72	326.5
73	261.0
74	197.5
75	169.0
76	154.5
77	138.5
78	104.0
79	71.0
80	69.5
81	74.0
82	68.0
83	56.0
84	43.5
85	27.5
86	21.0
87	18.0
88	14.5
89	7.0
90	6.0
91	6.5
92	4.0
93	3.0
94	1.0
95	0.5
96	1.0
97	1.0
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	215887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.83693877359723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47509429184925	72.49807185586812
2	10.724832410555587	17.982742363216794
3	1.7943178091363814	4.512903369148257
4	0.4639030100733089	1.5556883300961382
5	0.17999603683259519	0.7545158359712216
6	0.1114053221081838	0.5603928701182009
7	0.07951220686102753	0.4666242012853093
8	0.04544479553195428	0.3047962032472877
9	0.033752721572938564	0.2546752366757452
>10	0.09118890140380516	1.0827244754495646
>50	5.524940749792859E-4	0.026865258923351898
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	4.632052879515673E-4	0.0
21	0.0	0.0	0.0	0.001852821151806269	0.0
22	0.0	0.0	0.0	0.0032424370156609707	0.0
23	0.0	0.0	0.0	0.0032424370156609707	0.0
24	0.0	0.0	0.0	0.003705642303612538	0.0
25	0.0	0.0	0.0	0.003705642303612538	0.0
26	0.0	0.0	0.0	0.004168847591564106	0.0
27	0.0	0.0	0.0	0.004632052879515672	0.0
28	0.0	0.0	0.0	0.006948079319273509	0.0
29	0.0	0.0	0.0	0.011580132198789181	0.0
30	0.0	0.0	0.0	0.016675390366256423	0.0
31	0.0	0.0	0.0	0.018991416806014256	0.0
32	0.0	0.0	0.0	0.022697059109626796	0.0
33	0.0	0.0	0.0	0.027792317277094036	0.0
34	0.0	0.0	0.0	0.03659321774817381	0.0
35	0.0	0.0	0.0	0.05326860811443023	0.0
36	0.0	0.0	0.0	0.08476656769513681	0.0
37	0.0	0.0	0.0	0.1329399176420998	0.0
38	0.0	0.0	0.0	0.21446404832157565	0.0
39	0.0	0.0	0.0	0.30062023188056713	0.0
40	0.0	0.0	0.0	0.41086309041304014	0.0
41	0.0	0.0	0.0	0.5262012071129804	0.0
42	0.0	0.0	0.0	0.6841542103044649	0.0
43	0.0	0.0	0.0	0.8560033721344963	0.0
44	0.0	0.0	0.0	1.0639825464247499	0.0
45	0.0	0.0	0.0	1.3229143023896761	0.0
46	0.0	0.0	0.0	1.6198288919666306	0.0
47	0.0	0.0	0.0	1.9223019449990042	0.0
48	0.0	0.0	0.0	2.2358919249422153	0.0
49	0.0	0.0	0.0	2.551797931325184	0.0
50	0.0	0.0	0.0	2.8524181632057513	0.0
51	0.0	0.0	0.0	3.142847878751384	0.0
52	0.0	0.0	0.0	3.4388360577524355	0.0
53	0.0	0.0	0.0	3.7519628324076946	0.0
54	0.0	0.0	0.0	4.07574332868584	0.0
55	0.0	0.0	0.0	4.420368062921806	0.0
56	0.0	0.0	0.0	4.787226650979448	0.0
57	0.0	0.0	0.0	5.155474854900944	0.0
58	0.0	0.0	0.0	5.507510873744135	0.0
59	0.0	0.0	0.0	5.863252534890938	0.0
60	0.0	0.0	0.0	6.258366645513625	0.0
61	0.0	0.0	0.0	6.588632015823093	0.0
62	0.0	0.0	0.0	6.954100988016879	0.0
63	0.0	0.0	0.0	7.2894616164938135	0.0
64	0.0	0.0	0.0	7.649372125232182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCCG	50	0.007197606	27.999998	64
ATCAGGT	50	0.007197606	27.999998	12
AGGGCCG	50	0.007197606	27.999998	69
TGCCGCT	50	0.007197606	27.999998	66
ACGAGGG	90	6.1451665E-6	27.222223	69
CGAGGGC	110	1.0105105E-6	25.454548	70
GACCCAT	70	0.0012233292	25.0	6
GGTCTGG	70	0.0012233292	25.0	1
GGTTAGA	70	0.0012233292	25.0	1
ATCCATA	105	2.0306967E-5	23.333334	1
CTAGACT	80	0.0026602023	21.875002	4
CTACACC	80	0.0026602023	21.875002	4
CGACGAG	135	5.9874E-6	20.74074	69
GTCTGAG	85	0.0037809452	20.588236	1
ATAACAC	105	5.4468296E-4	20.000002	3
GTAACTT	105	5.4468296E-4	20.000002	38
CTGCCGA	380	0.0	19.342104	70
GACGAGG	145	1.1103057E-5	19.310345	70
GATCTAG	95	0.0071921153	18.421051	1
ACACCAT	160	2.591555E-5	17.500002	6
>>END_MODULE