FastQCFastQC Report
Wed 25 May 2016
SRR1781183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206113
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC252.3864204E-455.99999648
CGTAGTG200.006943984452.544
CGTATGC451.6790163E-646.66666441
TATGCCG451.6790163E-646.66666443
ATGCCGT451.6790163E-646.66666444
CCGTCTT404.5816276E-543.7547
TGCCGTC556.6725024E-638.18181645
AGCGTCA400.002414844735.03
ACCAGTT400.002414844735.037
TCGTATG400.002414844735.040
GCCGTCT501.705627E-435.046
GTATGCC751.4839807E-632.66666842
CTCGTAT450.00430034231.11110939
GTTATAC450.00430034231.1111093
CCATTAG450.00430034231.11110914
GTACTGA657.9370703E-426.9230791
GTGTAGG800.002659798421.8751
GAGTGTT850.003780373420.58823668
CTTACAC1205.6885794E-520.4166683
TATTGGG900.005262475519.4444432