FastQCFastQC Report
Wed 25 May 2016
SRR1781182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424687
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG503.4756868E-642.043
TAACCGG801.4206307E-939.37522
AACCGGC957.619747E-933.15789823
TACCGAA552.9903007E-431.8181811
TAAGGTA450.004305432631.1111096
TGGCGCT853.951269E-628.8235337
GGCGGTA853.951269E-628.8235341
GCGGTAT755.5798948E-528.042
TCTGCCG1154.8892616E-827.39130664
CGGCTTT1305.3896656E-926.92307726
ACCGGCT1207.386734E-826.2524
GTTCTAG808.658043E-526.251
ATCGAAG959.3796425E-625.78947451
GGCTTTT1251.09679604E-725.19999927
GTCCTTA851.3071217E-424.7058831
CGAACGA750.001831136623.33333414
TAGACGT1052.036704E-523.3333344
CCCGCTA1502.4690962E-823.33333417
TTGGCGG901.9260874E-423.33333239
CTTTTGG1352.3074244E-723.33333236
Produced by FastQC (version 0.11.5)