##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781182_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424687
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44020184276891	34.0	33.0	34.0	23.0	34.0
2	31.612790125433555	34.0	33.0	34.0	24.0	34.0
3	31.635762337910037	34.0	34.0	34.0	23.0	34.0
4	31.8135968372001	34.0	34.0	34.0	23.0	34.0
5	31.834190827597737	34.0	34.0	34.0	27.0	34.0
6	34.66419975181722	38.0	35.0	38.0	27.0	38.0
7	34.54573839086198	37.0	34.0	38.0	26.0	38.0
8	34.285730432059374	37.0	34.0	38.0	24.0	38.0
9	34.635246664013735	38.0	35.0	38.0	26.0	38.0
10-11	34.535709828650276	38.0	34.0	38.0	26.0	38.0
12-13	34.51273997084912	38.0	34.0	38.0	26.0	38.0
14-15	34.41076369184835	37.5	34.0	38.0	25.0	38.0
16-17	34.39297882911414	37.5	34.0	38.0	25.0	38.0
18-19	34.454619519787514	37.5	34.0	38.0	25.5	38.0
20-21	34.367628394558814	37.0	34.0	38.0	25.0	38.0
22-23	34.44505365127729	37.5	34.0	38.0	25.5	38.0
24-25	34.429384464323135	37.5	34.0	38.0	25.5	38.0
26-27	34.35962132111414	37.0	34.0	38.0	25.0	38.0
28-29	34.43472369062392	37.0	34.0	38.0	25.5	38.0
30-31	34.509418701302366	38.0	34.0	38.0	26.0	38.0
32-33	34.46738186005223	37.5	34.0	38.0	25.5	38.0
34-35	34.48979836915187	38.0	34.0	38.0	25.5	38.0
36-37	34.3474452950055	37.0	34.0	38.0	24.5	38.0
38-39	34.35050519559111	37.0	34.0	38.0	25.0	38.0
40-41	34.34506118623834	37.0	34.0	38.0	24.5	38.0
42-43	34.41636899646092	37.0	34.0	38.0	25.0	38.0
44-45	34.443108689458356	37.0	34.0	38.0	25.0	38.0
46-47	34.43264451231142	37.0	34.0	38.0	25.0	38.0
48-49	34.405282007690374	37.0	34.0	38.0	25.0	38.0
50-51	34.29022315258061	37.0	34.0	38.0	24.5	38.0
52-53	34.377269612679456	37.0	34.0	38.0	25.0	38.0
54-55	34.34159157214607	37.0	34.0	38.0	25.0	38.0
56-57	34.32146851681355	37.0	34.0	38.0	24.5	38.0
58-59	34.31913267889057	37.0	34.0	38.0	24.5	38.0
60-61	34.292171646412534	37.0	34.0	38.0	24.0	38.0
62-63	34.261352478413514	37.0	34.0	38.0	24.0	38.0
64-65	34.293225363620735	37.0	34.0	38.0	24.0	38.0
66-67	34.189364167021836	37.0	34.0	38.0	24.0	38.0
68-69	33.97078083388472	37.0	34.0	38.0	23.5	38.0
70-71	34.13371259303911	37.0	34.0	38.0	24.0	38.0
72-73	34.10256141581918	37.0	34.0	38.0	24.0	38.0
74-75	34.101043827571836	37.0	34.0	38.0	24.0	38.0
76	32.71324057482334	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	21.0
14	96.0
15	301.0
16	677.0
17	1260.0
18	2033.0
19	2235.0
20	2522.0
21	2880.0
22	3259.0
23	3860.0
24	4737.0
25	5540.0
26	6503.0
27	7991.0
28	9463.0
29	11235.0
30	13924.0
31	16550.0
32	20436.0
33	25323.0
34	32206.0
35	43192.0
36	65552.0
37	142889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6721938745476	19.09194300743842	10.34903352351261	25.886829594501364
2	22.406854930807864	19.953754176605358	32.748588960811134	24.890801931775638
3	19.75501957912533	22.206001125534804	28.99806210220704	29.040917193132827
4	14.149244031486718	16.06971722704015	37.019028131306115	32.76201061016702
5	14.030332927544286	34.333285454935044	35.70182275416954	15.934558863351128
6	30.905584583469707	36.67077165065095	17.978887981030738	14.444755784848606
7	29.750145401201355	28.729864582622028	22.492800580192	19.02718943598462
8	26.06201743872546	34.17669954578313	20.032635800012716	19.72864721547869
9	24.903752646066398	16.494736123309636	21.90012880074031	36.70138242988366
10-11	25.751671230812338	24.5603232498287	27.405830647041235	22.282174872317732
12-13	25.407417698210683	22.91605346996729	27.001061958571842	24.675466873250183
14-15	23.324118703892513	24.205355944495594	24.169447145780303	28.301078205831587
16-17	23.579718710485604	27.326360354802475	25.435438334585236	23.658482600126682
18-19	23.154817547982397	26.67729410130284	26.815395809148857	23.352492541565905
20-21	23.078290599900633	26.851657809162983	26.68777240650173	23.382279184434655
22-23	23.53333160657143	26.358353328451305	26.835646016948957	23.272669048028312
24-25	23.167768262273157	26.576749464900036	26.78702197147546	23.46846030135135
26-27	22.430637151596354	26.787610640306863	27.101371127442093	23.680381080654694
28-29	22.81621523616216	26.66457885454464	27.717000991318315	22.802204917974887
30-31	23.187429801241855	26.45183393887734	27.114675043031696	23.246061216849114
32-33	22.885913625799706	26.84871446500599	26.7816062182266	23.4837656909677
34-35	23.494832664997986	26.076380958211576	27.303755471676787	23.125030905113647
36-37	23.3542585480601	26.22790431541347	26.816926348110492	23.600910788415938
38-39	22.82304379460638	26.740870335093845	27.31847219246174	23.11761367783803
40-41	22.970917405053605	25.82549030226967	27.9338665888054	23.269725703871323
42-43	22.714846463395393	25.728359945088968	27.667670543247148	23.88912304826849
44-45	22.23366856061052	25.623459159333816	27.932100582311207	24.210771697744455
46-47	22.336214671040082	25.204562418910868	28.409393270808852	24.049829639240194
48-49	21.728943904569718	25.161707327985084	29.099901810038926	24.009446957406276
50-51	21.558112209697967	25.2153939254086	28.993588219088412	24.23290564580503
52-53	21.08435153418871	25.225519029308646	29.3214767581772	24.36865267832545
54-55	20.465778326155498	25.76179633471239	29.260843868543184	24.51158147058893
56-57	20.4061593814717	25.79850265667182	29.198312633701878	24.597025328154597
58-59	20.57569660372205	25.65569109376232	28.798958287956616	24.96965401455901
60-61	20.231982886199585	25.58640314679181	28.81961164294132	25.362002324067284
62-63	20.05599431109102	25.289390278935496	29.104797426804748	25.549817983168744
64-65	20.816791700220868	25.267138544713035	28.31586160127718	25.600208153788916
66-67	20.948560758490693	25.113525185107566	27.801278828686172	26.136635227715566
68-69	20.908531136570474	25.028756573982392	27.404161432401153	26.658550857045977
70-71	21.462680662889756	25.04285519183585	27.338904508271995	26.155559637002394
72-73	21.326109172423863	25.11137640515581	26.995827505498184	26.56668691692215
74-75	21.370023970651257	25.716411654728432	26.69890224777836	26.214662126841947
76	21.806648190314277	25.754025906137933	26.416867010292282	26.0224588932555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	2.0
13	9.5
14	17.5
15	28.0
16	61.5
17	85.0
18	127.5
19	203.5
20	339.0
21	441.0
22	531.5
23	796.5
24	1252.5
25	1534.0
26	1837.0
27	2457.0
28	3168.5
29	3563.0
30	4148.5
31	5302.5
32	7032.0
33	8193.0
34	8678.0
35	10348.0
36	13037.5
37	14542.0
38	15820.0
39	18539.5
40	21521.5
41	24076.5
42	25091.0
43	25506.5
44	26367.5
45	26831.0
46	26849.0
47	26186.5
48	24122.0
49	21716.5
50	20713.0
51	19273.5
52	16285.0
53	13537.5
54	12339.0
55	11347.0
56	9550.0
57	7975.5
58	7206.0
59	6540.5
60	5428.5
61	4726.0
62	4470.0
63	4225.0
64	3756.5
65	3397.5
66	3199.5
67	3137.0
68	3143.5
69	3015.5
70	2840.5
71	2800.0
72	2702.5
73	2433.5
74	2170.0
75	2078.0
76	1956.0
77	1699.0
78	1444.5
79	1325.0
80	1168.5
81	894.0
82	639.0
83	502.0
84	455.5
85	314.5
86	188.5
87	157.0
88	123.5
89	69.5
90	43.5
91	29.0
92	20.0
93	17.0
94	12.0
95	7.0
96	4.0
97	2.5
98	1.5
99	4.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.532012988389096E-4
58-59	3.532012988389096E-4
60-61	1.1773376627963652E-4
62-63	2.3546753255927305E-4
64-65	2.3546753255927305E-4
66-67	3.532012988389096E-4
68-69	3.532012988389096E-4
70-71	2.3546753255927305E-4
72-73	2.3546753255927305E-4
74-75	2.3546753255927305E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	424687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51986364286357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59341072597795	35.45295936877954
2	15.030692943247074	14.886357300146935
3	5.728502413387184	8.510239751662448
4	2.2474616937618563	4.451759864705854
5	1.0817729112803613	2.6784623529573524
6	0.6340171317022222	1.883786514548011
7	0.4745464693213782	1.644963351709789
8	0.36104073813333537	1.4302950497505265
9	0.30809589671821774	1.373118011497073
>10	2.396451178036848	22.818499881011544
>50	0.13399410417166843	4.255609749120921
>100	0.01001379426189193	0.6139488041100488
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3546753255927308E-4	0.0
2	0.0	0.0	0.0	2.3546753255927308E-4	0.0
3	0.0	0.0	0.0	2.3546753255927308E-4	0.0
4	0.0	0.0	0.0	7.064025976778192E-4	0.0
5	0.0	0.0	0.0	9.418701302370923E-4	0.0
6	0.0	0.0	0.0	0.0011773376627963654	0.0
7	0.0	0.0	0.0	0.0011773376627963654	0.0
8	0.0	0.0	0.0	0.0014128051953556384	0.0
9	0.0	0.0	0.0	0.0018837402604741846	0.0
10	0.0	0.0	0.0	0.0018837402604741846	0.0
11	0.0	0.0	0.0	0.002354675325592731	0.0
12	0.0	0.0	0.0	0.0025901428581520036	0.0
13	0.0	0.0	0.0	0.0025901428581520036	0.0
14	0.0	0.0	0.0	0.0025901428581520036	0.0
15	0.0	0.0	0.0	0.0025901428581520036	0.0
16	0.0	0.0	0.0	0.0025901428581520036	0.0
17	0.0	0.0	0.0	0.00306107792327055	0.0
18	0.0	0.0	0.0	0.003296545455829823	0.0
19	0.0	0.0	0.0	0.004002948053507642	0.0
20	0.0	0.0	0.0	0.004238415586066915	0.0
21	0.0	0.0	0.0	0.005180285716304007	0.0
22	0.0	0.0	0.0	0.0061221558465411	0.0
23	0.0	0.0	0.0	0.0061221558465411	0.0
24	0.0	0.0	0.0	0.006593090911659646	0.0
25	0.0	0.0	0.0	0.006828558444218919	0.0
26	0.0	0.0	0.0	0.007534961041896738	0.0
27	0.0	0.0	0.0	0.018837402604741846	0.0
28	0.0	0.0	0.0	0.027314233776875675	0.0
29	0.0	0.0	0.0	0.04073588313275424	0.0
30	0.0	0.0	0.0	0.056983142879344084	0.0
31	0.0	0.0	0.0	0.07582054548408593	0.0
32	0.0	0.0	0.0	0.09512888315394631	0.0
33	0.0	0.0	0.0	0.12621059745177035	0.0
34	0.0	0.0	0.0	0.18083906500552172	0.0
35	0.0	0.0	0.0	0.2632527014012673	0.0
36	0.0	0.0	0.0	0.40712336379498315	0.0
37	0.0	0.0	0.0	0.6557770781775755	0.0
38	0.0	0.0	0.0	0.9790740003814574	0.0
39	0.0	0.0	0.0	1.4015027537927933	0.0
40	0.0	0.0	0.0	1.890097883853285	0.0
41	0.0	0.0	0.0	2.4045944424952967	0.0
42	0.0	0.0	0.0	2.9932632738934792	0.0
43	0.0	0.0	0.0	3.742756430029645	0.0
44	0.0	0.0	0.0	4.5478199238498	0.0
45	0.0	0.0	0.0	5.48403883330547	0.0
46	0.0	0.0	0.0	6.5306920155314385	0.0
47	0.0	0.0	0.0	7.544615210731668	0.0
48	0.0	0.0	0.0	8.545823159173697	0.0
49	0.0	0.0	0.0	9.465323873817658	0.0
50	0.0	0.0	0.0	10.299114406610045	0.0
51	0.0	0.0	0.0	11.151271406942055	0.0
52	0.0	0.0	0.0	12.058292342360375	0.0
53	0.0	0.0	0.0	12.893495680348115	0.0
54	0.0	0.0	0.0	13.811348122264162	0.0
55	0.0	0.0	0.0	14.798428018752634	0.0
56	0.0	0.0	0.0	15.731114915219916	0.0
57	0.0	0.0	0.0	16.632013694791695	0.0
58	0.0	0.0	0.0	17.49782781201214	0.0
59	0.0	0.0	0.0	18.287585916215942	0.0
60	0.0	0.0	0.0	19.106541994457093	0.0
61	0.0	0.0	0.0	19.861450903842123	0.0
62	0.0	0.0	0.0	20.629075059985354	0.0
63	0.0	0.0	0.0	21.37503620313313	0.0
64	0.0	0.0	0.0	22.1360672683647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATG	50	3.4756868E-6	42.0	43
TAACCGG	80	1.4206307E-9	39.375	22
AACCGGC	95	7.619747E-9	33.157898	23
TACCGAA	55	2.9903007E-4	31.81818	11
TAAGGTA	45	0.0043054326	31.111109	6
TGGCGCT	85	3.951269E-6	28.82353	37
GGCGGTA	85	3.951269E-6	28.82353	41
GCGGTAT	75	5.5798948E-5	28.0	42
TCTGCCG	115	4.8892616E-8	27.391306	64
CGGCTTT	130	5.3896656E-9	26.923077	26
ACCGGCT	120	7.386734E-8	26.25	24
GTTCTAG	80	8.658043E-5	26.25	1
ATCGAAG	95	9.3796425E-6	25.789474	51
GGCTTTT	125	1.09679604E-7	25.199999	27
GTCCTTA	85	1.3071217E-4	24.705883	1
CGAACGA	75	0.0018311366	23.333334	14
TAGACGT	105	2.036704E-5	23.333334	4
CCCGCTA	150	2.4690962E-8	23.333334	17
TTGGCGG	90	1.9260874E-4	23.333332	39
CTTTTGG	135	2.3074244E-7	23.333332	36
>>END_MODULE