FastQCFastQC Report
Wed 25 May 2016
SRR1781182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382112
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT31060.8128506825224018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4550.053.84615738
TATGCCG5300.053.49056643
TCGTATG5300.053.49056640
CTCGTAT5150.053.0097139
CCGTCTT5150.053.0097147
CGTATGC5350.052.99065441
ATCTCGT4650.052.6881737
TATCGTG200.006948502752.50000437
CGTCTTC5200.051.82692348
GATCTCG5050.048.5148536
GCCGTCT5700.047.89473746
GTATGCC5950.047.6470642
ACAGATC5250.047.33333233
TGCCGTC5950.046.4705945
CTTGAAA5800.046.46551557
ATGCCGT6050.046.2809944
AGATCTC5600.043.7535
GTCTTCT6100.043.6065649
CAGATCT5700.042.98245634
CTGCTTG6350.041.88976754
Produced by FastQC (version 0.11.5)