##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781182_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382112
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.30792280797253	34.0	34.0	34.0	33.0	34.0
2	33.30944068754711	34.0	34.0	34.0	33.0	34.0
3	33.38610930826564	34.0	34.0	34.0	33.0	34.0
4	33.46184364793569	34.0	34.0	34.0	34.0	34.0
5	33.44977127125031	34.0	34.0	34.0	34.0	34.0
6	36.8710796834436	38.0	37.0	38.0	34.0	38.0
7	36.76625177958295	38.0	37.0	38.0	34.0	38.0
8	36.586189389498365	38.0	37.0	38.0	34.0	38.0
9	36.53645528012729	38.0	37.0	38.0	34.0	38.0
10-11	36.46118022988024	38.0	37.0	38.0	34.0	38.0
12-13	36.411574355162884	38.0	37.0	38.0	34.0	38.0
14-15	36.4264508835106	38.0	37.0	38.0	34.0	38.0
16-17	36.376852859894484	38.0	37.0	38.0	34.0	38.0
18-19	36.39247262582698	38.0	37.0	38.0	34.0	38.0
20-21	36.32113621137258	38.0	37.0	38.0	34.0	38.0
22-23	36.37547760865924	38.0	37.0	38.0	34.0	38.0
24-25	36.36524500669961	38.0	37.0	38.0	34.0	38.0
26-27	36.17711691859978	38.0	37.0	38.0	33.5	38.0
28-29	36.16421625073277	38.0	37.0	38.0	34.0	38.0
30-31	36.18780357591491	38.0	37.0	38.0	34.0	38.0
32-33	36.16032210451386	38.0	37.0	38.0	34.0	38.0
34-35	36.162686594506326	38.0	37.0	38.0	34.0	38.0
36-37	36.119578814588394	38.0	37.0	38.0	33.5	38.0
38-39	36.04781058956536	38.0	37.0	38.0	32.5	38.0
40-41	36.085734025625996	38.0	37.0	38.0	33.0	38.0
42-43	36.08868473117829	38.0	37.0	38.0	33.0	38.0
44-45	36.07096610417888	38.0	37.0	38.0	32.5	38.0
46-47	36.08684626496944	38.0	37.0	38.0	32.5	38.0
48-49	36.07405420400302	38.0	37.0	38.0	32.5	38.0
50-51	35.98877161669877	38.0	37.0	38.0	32.0	38.0
52-53	36.025304099321666	38.0	37.0	38.0	32.0	38.0
54-55	36.03191472657231	38.0	37.0	38.0	32.0	38.0
56-57	35.98572408089775	38.0	37.0	38.0	32.0	38.0
58-59	35.9912564902437	38.0	37.0	38.0	32.0	38.0
60-61	35.98304816388912	38.0	37.0	38.0	32.0	38.0
62-63	35.960150427099904	38.0	37.0	38.0	32.0	38.0
64-65	35.91578385394858	38.0	37.0	38.0	32.0	38.0
66-67	35.887297441587805	38.0	37.0	38.0	32.0	38.0
68-69	35.82340779666694	38.0	36.5	38.0	31.0	38.0
70-71	35.765910256678666	38.0	36.5	38.0	31.0	38.0
72-73	35.72604890712671	38.0	36.5	38.0	31.0	38.0
74-75	35.74650495142785	38.0	37.0	38.0	31.0	38.0
76	34.836563625324516	37.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	17.0
22	32.0
23	114.0
24	222.0
25	441.0
26	855.0
27	1525.0
28	2652.0
29	4330.0
30	7003.0
31	10361.0
32	15316.0
33	21265.0
34	30120.0
35	42503.0
36	66230.0
37	179120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.328688970772966	19.873230885185496	10.823266476844486	26.97481366719705
2	21.504689724478688	20.072125450129803	33.0489489992463	25.374235826145213
3	19.62775311950423	22.72004019763839	28.7557051335734	28.896501549283983
4	14.095343773553303	16.988474583368227	36.606806381375094	32.30937526170338
5	14.014215727326022	34.96697303408425	35.726697931496524	15.292113307093208
6	31.173582614521393	37.58636211372582	17.1625073276945	14.077547944058285
7	29.456023364877314	29.022904279373584	21.60675404070011	19.91431831504899
8	25.600085838706978	35.40061552633782	19.089690980654886	19.90960765430031
9	24.301513692320576	16.12982581023365	21.157671049325852	38.41098944811993
10-11	25.358664475337072	25.415584959383636	26.916584666275856	22.309165899003432
12-13	25.03075014655389	23.067843982916003	26.704735784272675	25.196670086257434
14-15	22.697402855707228	24.308579683443597	23.68886609161712	29.305151369232057
16-17	23.672117075621806	27.811741060212714	24.922404949334226	23.593736914831254
18-19	23.48460660748681	26.780237207939035	26.052701825642743	23.682454358931412
20-21	22.72867640901097	26.96146679507579	25.89136169500042	24.41849510091282
22-23	23.01040009211959	26.988945649443096	26.34934260112218	23.651311657315134
24-25	23.35009107277447	27.064577924796918	26.24296017921447	23.342370823214136
26-27	22.68693472071016	27.26203312117913	26.579510719370237	23.471521438740474
28-29	22.726844485386483	26.98632861569383	27.10121639728666	23.18561050163303
30-31	22.79554162130475	27.703788418055442	26.39775772548363	23.102912235156182
32-33	22.260096516204673	27.59779855121012	26.894209027719622	23.24789590486559
34-35	23.148056067331044	26.797771334059124	26.59913847248974	23.455034126120093
36-37	23.56638891215141	26.914098484214055	26.16981408592245	23.349698517712085
38-39	22.42915689640734	26.887404739971526	27.253266058119085	23.43017230550205
40-41	22.517220082070178	25.658838246378025	27.921002219244617	23.902939452307177
42-43	22.924168830081232	25.733423708232138	27.557627083158863	23.784780378527763
44-45	21.736689766351226	26.08868603969517	28.055910309019342	24.11871388493426
46-47	22.470244326270834	24.999869148312538	27.87939138263127	24.65049514278536
48-49	21.887300058621555	24.916254920023448	28.59096285905703	24.605482162297964
50-51	21.01595867180303	25.087278075538062	29.011781885939204	24.884981366719707
52-53	20.700475253328868	25.09761535884767	29.862710409513443	24.339198978310026
54-55	19.98667929821623	25.67467130056109	29.339827066409846	24.99882233481283
56-57	20.34521292186584	25.473552256929903	28.761593459509253	25.419641361695
58-59	19.777447449962317	25.33707394690562	29.358538857717107	25.526939745414957
60-61	20.096333012310527	26.01475483627837	28.22824198140859	25.660670170002515
62-63	19.195811699187672	26.128203249309102	28.164909764676327	26.511075286826895
64-65	19.491798216229796	26.363343731680764	27.35977933171426	26.78507872037518
66-67	19.761876099154176	26.370802277866172	26.620336445858804	27.24698517712084
68-69	19.933815216481033	26.732476342014905	25.901698978310023	27.432009463194035
70-71	19.97895904865589	26.81923101080311	25.60060924545683	27.601200695084167
72-73	19.813300812327274	26.48765283477096	25.43874570806465	28.260300644837116
74-75	19.897831002428607	27.1853540323256	25.061761996482705	27.855052968763083
76	20.268664684699775	27.62881040113893	24.689619797336906	27.412905116824387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	4.5
14	5.5
15	20.5
16	62.5
17	91.0
18	127.5
19	209.5
20	312.0
21	369.0
22	492.0
23	812.0
24	1257.5
25	1506.0
26	1748.0
27	2211.5
28	2873.5
29	3314.0
30	3742.5
31	4655.5
32	6158.0
33	7176.0
34	7744.5
35	9340.5
36	11534.5
37	12701.0
38	14029.0
39	16960.0
40	19885.5
41	22040.5
42	22873.0
43	23057.0
44	23409.5
45	23574.0
46	23570.0
47	23123.0
48	21535.0
49	19616.0
50	18838.0
51	17565.0
52	14660.5
53	12186.5
54	11344.0
55	10278.5
56	8585.5
57	7131.5
58	6305.0
59	5700.0
60	4781.0
61	4179.5
62	3892.0
63	3597.0
64	3182.0
65	3057.5
66	3007.5
67	2962.0
68	2882.0
69	2712.5
70	2615.5
71	2608.0
72	2517.5
73	2278.5
74	2044.5
75	1959.0
76	1877.0
77	1647.5
78	1361.0
79	1222.0
80	1093.5
81	847.0
82	626.0
83	523.0
84	441.0
85	301.0
86	190.5
87	138.0
88	102.5
89	57.0
90	35.5
91	15.5
92	7.0
93	5.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	382112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.24238982602762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.94814687974453	26.168377694941846
2	20.381648519945394	17.219390841532242
3	7.73628708951833	9.803977651244937
4	3.0061879499660717	5.0795425309109445
5	1.3511765440269476	2.853846314828554
6	0.8442596390024003	2.1398126871072445
7	0.5857324989475801	1.7319918388021889
8	0.4658652507321627	1.5743409222262492
9	0.38074956506366353	1.4475394398158767
>10	3.097859050240656	25.13716009481069
>50	0.18529824559406483	5.0933524526198495
>100	0.016166961024985518	0.9348275684015287
>500	0.0	0.0
>1k	6.218061932686738E-4	0.8158399627578389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	3106	0.8128506825224018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.6170337492672303E-4	0.0
2	0.0	0.0	0.0	2.6170337492672303E-4	0.0
3	0.0	0.0	0.0	2.6170337492672303E-4	0.0
4	0.0	0.0	0.0	7.851101247801692E-4	0.0
5	0.0	0.0	0.0	0.0010468134997068921	0.0
6	0.0	0.0	0.0	0.0013085168746336153	0.0
7	0.0	0.0	0.0	0.0013085168746336153	0.0
8	0.0	0.0	0.0	0.0015702202495603383	0.0
9	0.0	0.0	0.0	0.0020936269994137843	0.0
10	0.0	0.0	0.0	0.0020936269994137843	0.0
11	0.0	0.0	0.0	0.0023553303743405075	0.0
12	0.0	0.0	0.0	0.0023553303743405075	0.0
13	0.0	0.0	0.0	0.0023553303743405075	0.0
14	0.0	0.0	0.0	0.0026170337492672307	0.0
15	0.0	0.0	0.0	0.0026170337492672307	0.0
16	0.0	0.0	0.0	0.0026170337492672307	0.0
17	0.0	0.0	0.0	0.0028787371241939534	0.0
18	0.0	0.0	0.0	0.0031404404991206766	0.0
19	0.0	0.0	0.0	0.0036638472489741226	0.0
20	0.0	0.0	0.0	0.003925550623900846	0.0
21	0.0	0.0	0.0	0.004448957373754292	0.0
22	0.0	0.0	0.0	0.005234067498534461	0.0
23	0.0	0.0	0.0	0.005495770873461184	0.0
24	0.0	0.0	0.0	0.005757474248387907	0.0
25	0.0	0.0	0.0	0.0060191776233146305	0.0
26	0.0	0.0	0.0	0.007065991123021522	0.0
27	0.0	0.0	0.0	0.019366049744577507	0.0
28	0.0	0.0	0.0	0.028525667867012813	0.0
29	0.0	0.0	0.0	0.04291935348798258	0.0
30	0.0	0.0	0.0	0.0612385897328532	0.0
31	0.0	0.0	0.0	0.08086634285235743	0.0
32	0.0	0.0	0.0	0.10127920609664182	0.0
33	0.0	0.0	0.0	0.1373942718365296	0.0
34	0.0	0.0	0.0	0.1986328615693828	0.0
35	0.0	0.0	0.0	0.289182229294029	0.0
36	0.0	0.0	0.0	0.4462042542500628	0.0
37	0.0	0.0	0.0	0.7128799933003936	0.0
38	0.0	0.0	0.0	1.0520475672054266	0.0
39	0.0	0.0	0.0	1.4972050079557826	0.0
40	0.0	0.0	0.0	2.0104053261870867	0.0
41	0.0	0.0	0.0	2.5557951595343775	0.0
42	0.0	0.0	0.0	3.1823130391089522	0.0
43	0.0	0.0	0.0	3.969778494263462	0.0
44	0.0	0.0	0.0	4.81089314127795	0.0
45	0.0	0.0	0.0	5.80274893225023	0.0
46	0.0	0.0	0.0	6.90085629344276	0.0
47	0.0	0.0	0.0	7.956306004522235	0.0
48	0.0	0.0	0.0	8.991604555732351	0.0
49	0.0	0.0	0.0	9.945513357340257	0.0
50	0.0	0.0	0.0	10.797619546101666	0.0
51	0.0	0.0	0.0	11.683223766853697	0.0
52	0.0	0.0	0.0	12.62378569634034	0.0
53	0.0	0.0	0.0	13.491594087597354	0.0
54	0.0	0.0	0.0	14.449951846579014	0.0
55	0.0	0.0	0.0	15.459341763671384	0.0
56	0.0	0.0	0.0	16.432878318398792	0.0
57	0.0	0.0	0.0	17.40196591575245	0.0
58	0.0	0.0	0.0	18.2907105770036	0.0
59	0.0	0.0	0.0	19.137059291516625	0.0
60	0.0	0.0	0.0	19.965873879909555	0.0
61	0.0	0.0	0.0	20.74103927644251	0.0
62	0.0	0.0	0.0	21.541851603718282	0.0
63	0.0	0.0	0.0	22.316231890126456	0.0
64	0.0	0.0	0.0	23.11782932752701	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	455	0.0	53.846157	38
TATGCCG	530	0.0	53.490566	43
TCGTATG	530	0.0	53.490566	40
CTCGTAT	515	0.0	53.00971	39
CCGTCTT	515	0.0	53.00971	47
CGTATGC	535	0.0	52.990654	41
ATCTCGT	465	0.0	52.68817	37
TATCGTG	20	0.0069485027	52.500004	37
CGTCTTC	520	0.0	51.826923	48
GATCTCG	505	0.0	48.51485	36
GCCGTCT	570	0.0	47.894737	46
GTATGCC	595	0.0	47.64706	42
ACAGATC	525	0.0	47.333332	33
TGCCGTC	595	0.0	46.47059	45
CTTGAAA	580	0.0	46.465515	57
ATGCCGT	605	0.0	46.28099	44
AGATCTC	560	0.0	43.75	35
GTCTTCT	610	0.0	43.60656	49
CAGATCT	570	0.0	42.982456	34
CTGCTTG	635	0.0	41.889767	54
>>END_MODULE