FastQCFastQC Report
Wed 25 May 2016
SRR1781181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131007
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA751.4918078E-632.66666870
TATACCG657.959256E-426.9230776
AAACGGT700.001226542525.00000220
AACCGTA901.9288462E-423.33333210
ATCCTCG1102.9234057E-522.27272834
TATCGGC952.7813684E-422.10526315
GGTATAC1506.385162E-721.0000021
CGTTCGG850.003790728320.58823624
GTATCGG1055.466485E-420.00000214
TCTACGC900.00527681419.4444433
TATCGGT900.00527681419.44444324
TATGCGG1451.1171436E-519.31034543
CGTATCG1107.4770185E-419.0909113
ATACCGC1107.4770185E-419.090917
CATATCG1301.0576176E-418.84615358
CGCCATA1501.4964369E-518.66666855
CAACCGT1150.001008004418.2608689
CGATAAA17200.017.7034970
GTAGCGA1000.00969027817.50000249
AGCGTTC1200.001341050517.527
Produced by FastQC (version 0.11.5)