##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781181_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131007
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889960009089247	34.0	34.0	34.0	27.0	34.0
2	31.79540887014846	34.0	34.0	34.0	24.0	34.0
3	31.807189522257598	34.0	34.0	34.0	27.0	34.0
4	31.977901993533198	34.0	34.0	34.0	27.0	34.0
5	31.980478458577178	34.0	34.0	34.0	27.0	34.0
6	34.92430639244496	38.0	35.0	38.0	27.0	38.0
7	34.799388509531774	38.0	35.0	38.0	27.0	38.0
8	34.652362894305696	38.0	35.0	38.0	27.0	38.0
9	34.92635235679355	38.0	36.0	38.0	27.0	38.0
10-11	34.815884428655174	38.0	35.0	38.0	27.0	38.0
12-13	34.772919177334884	38.0	35.0	38.0	27.0	38.0
14-15	34.66381242556412	38.0	35.0	38.0	26.5	38.0
16-17	34.65729522452116	38.0	34.5	38.0	26.5	38.0
18-19	34.719137901003265	38.0	35.0	38.0	27.0	38.0
20-21	34.64442218306341	38.0	34.5	38.0	26.5	38.0
22-23	34.72684430777175	38.0	35.0	38.0	26.5	38.0
24-25	34.71169188165944	38.0	35.0	38.0	26.5	38.0
26-27	34.64084528212469	38.0	35.0	38.0	26.5	38.0
28-29	34.73015419002712	38.0	35.0	38.0	27.0	38.0
30-31	34.806470693815335	38.0	35.0	38.0	27.0	38.0
32-33	34.763726926535384	38.0	35.0	38.0	27.0	38.0
34-35	34.79206362118006	38.0	35.0	38.0	27.0	38.0
36-37	34.65101498045547	38.0	35.0	38.0	26.5	38.0
38-39	34.65558966478545	38.0	35.0	38.0	26.0	38.0
40-41	34.64026305761149	38.0	35.0	38.0	26.5	38.0
42-43	34.71927936785537	38.0	35.0	38.0	27.0	38.0
44-45	34.731116606705356	38.0	35.0	38.0	27.0	38.0
46-47	34.727796114436075	38.0	35.0	38.0	27.0	38.0
48-49	34.698290549925865	38.0	35.0	38.0	27.0	38.0
50-51	34.57584524233714	37.5	34.5	38.0	26.0	38.0
52-53	34.65670548458144	38.0	35.0	38.0	27.0	38.0
54-55	34.61811509566254	38.0	35.0	38.0	26.0	38.0
56-57	34.59346272834739	37.5	34.5	38.0	26.0	38.0
58-59	34.59774828979839	37.5	35.0	38.0	26.0	38.0
60-61	34.578658222274484	37.5	34.5	38.0	26.0	38.0
62-63	34.55121321088198	37.0	34.5	38.0	26.0	38.0
64-65	34.56388510415939	37.0	35.0	38.0	26.0	38.0
66-67	34.47644444287259	37.0	34.0	38.0	25.0	38.0
68-69	34.27646336406406	37.0	34.0	38.0	24.5	38.0
70-71	34.430770101334474	37.0	34.0	38.0	25.0	38.0
72-73	34.408767584992844	37.0	34.0	38.0	25.0	38.0
74-75	34.41745453387999	37.0	34.0	38.0	25.0	38.0
76	33.165277491651246	37.0	32.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	43.0
14	149.0
15	449.0
16	1007.0
17	2028.0
18	3020.0
19	3955.0
20	4857.0
21	5725.0
22	7197.0
23	9011.0
24	11134.0
25	13717.0
26	16655.0
27	20099.0
28	24630.0
29	29533.0
30	36031.0
31	43284.0
32	53773.0
33	66576.0
34	85471.0
35	113398.0
36	175087.0
37	404170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.22136379350437	18.493873159052065	9.40480474479822	23.879958302645342
2	22.942033073181687	22.250260166382702	30.672577623303837	24.135129137131777
3	19.516059582301438	22.67006305000765	30.869658631644192	26.944218736046725
4	13.996730347380698	17.576902707056632	37.221520291209515	31.204846654353158
5	13.895227880994549	33.41314421572988	36.021439301436686	16.67018860183889
6	28.81635569010625	37.26838118596967	19.789709524344236	14.125553599579844
7	27.463048416145963	31.06063888198747	22.43239873846935	19.043913963397223
8	24.74361343475328	35.63841779935933	21.182715933676803	18.435252832210587
9	24.545736675369824	18.025529461798204	22.441770917421376	34.986962945410596
10-11	24.412713625998776	25.475262310489676	28.40070839526192	21.711315668249622
12-13	25.1036023649721	23.838667665186865	27.53002412893996	23.527705840901074
14-15	22.908125237067498	24.659661699706543	25.132868320001556	27.299344743224403
16-17	22.780981903737114	28.233954343341814	25.76155585243946	23.223507900481607
18-19	21.965602334910393	27.79943006541958	27.688245961342417	22.546721638327615
20-21	22.307731075050818	27.912249879974215	27.428963746466646	22.35105529850832
22-23	22.279889638123212	27.71756837825424	27.603775928785556	22.398766054836997
24-25	22.45980794106491	27.557875415448358	27.36773512453946	22.614581518947276
26-27	22.042480727351823	27.43462242055089	27.495276333391395	23.027620518705895
28-29	21.909634511545907	27.542446686890532	28.06392887046676	22.4839899310968
30-31	22.126344045615987	27.842533246920663	27.64337444419	22.387748263273348
32-33	21.800705035424183	28.004070708669353	27.767732648869547	22.427491607036913
34-35	22.24981808246987	27.321227896909566	28.080683850763084	22.348270169857482
36-37	22.389118723403126	27.548459028105043	27.46914917414304	22.593273074348787
38-39	22.013922106582896	27.559157458795568	27.948721802782828	22.478198631838705
40-41	22.042657560916954	27.187409096495426	28.28169940592764	22.488233936659984
42-43	21.743057293190933	27.145278499602565	28.258445792112692	22.853218415093806
44-45	21.686382135565914	26.808410558024843	28.44690616415283	23.05830114225641
46-47	21.754639891707125	26.699923165815946	28.503802363734266	23.04163457874266
48-49	21.22303398652705	26.684361812084273	29.023516211659167	23.069087989729507
50-51	21.303139591532148	26.508058747647006	28.93275638435483	23.25604527646602
52-53	20.906280862983166	26.46827119549216	29.310030795565368	23.31541714595931
54-55	20.641118932066732	26.702619877684224	29.281162716057462	23.37509847419158
56-57	20.565072656619556	26.661199552610288	29.305750195622476	23.46797759514768
58-59	20.302200121926905	26.59102895609261	29.340396664737707	23.76637425724278
60-61	20.412323370664424	26.59845597567828	28.924748818639696	24.0644718350176
62-63	20.23566640480528	26.57564441552229	29.053749075490792	24.13494010418163
64-65	20.616301158570845	26.176442112792554	28.88367916866894	24.323577559967656
66-67	20.631429569276882	26.13047687676005	28.52790217549878	24.710191378464287
68-69	20.87139314149805	26.101741371611975	28.180246771676522	24.846618715213463
70-71	21.253884819253674	26.20271351585537	27.895367394485437	24.648034270405525
72-73	21.017723175405944	26.24563993969965	27.884005817834577	24.85263106705983
74-75	21.068253455997098	26.41637304874868	27.62176117700629	24.89361231824793
76	21.15070905838779	26.535556367025137	27.292227192227813	25.02150738235926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	2.5
8	5.0
9	4.5
10	5.5
11	12.5
12	18.0
13	27.5
14	47.5
15	115.0
16	279.0
17	386.0
18	536.0
19	936.5
20	1565.5
21	1944.0
22	2577.0
23	3964.0
24	5827.5
25	6937.0
26	8208.0
27	11062.0
28	14362.0
29	16079.0
30	18335.5
31	22627.0
32	27163.5
33	29665.0
34	32095.0
35	37090.5
36	42370.0
37	45084.0
38	47583.0
39	52441.0
40	56667.5
41	59462.5
42	60390.0
43	60649.0
44	60809.0
45	60374.0
46	60038.0
47	58135.5
48	54132.5
49	49917.5
50	47803.0
51	45334.0
52	40616.0
53	36417.5
54	34468.0
55	32719.0
56	29101.0
57	25501.0
58	23770.0
59	21812.5
60	18196.0
61	14942.5
62	13348.0
63	12159.0
64	10034.5
65	8429.5
66	7116.0
67	6472.0
68	6138.5
69	5394.5
70	4607.5
71	4231.0
72	3970.5
73	3533.0
74	3112.5
75	2869.0
76	2729.5
77	2379.0
78	1960.0
79	1752.0
80	1552.0
81	1203.5
82	896.0
83	737.0
84	626.0
85	425.0
86	266.0
87	197.0
88	165.0
89	110.5
90	68.5
91	39.5
92	30.0
93	23.0
94	9.5
95	3.5
96	4.0
97	5.0
98	5.0
99	10.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.420839128316624E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.7683356513266497E-4
58-59	1.3262517384949872E-4
60-61	1.3262517384949872E-4
62-63	1.3262517384949872E-4
64-65	1.3262517384949872E-4
66-67	1.7683356513266497E-4
68-69	1.7683356513266497E-4
70-71	1.7683356513266497E-4
72-73	1.7683356513266497E-4
74-75	1.7683356513266497E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1131007.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.91191952109505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16507738165623	44.77131620947258
2	18.604007189075364	23.408276060979663
3	6.238212110691916	11.77373694790111
4	1.9437307863431514	4.891353392043805
5	0.6160461547665754	1.937832305497743
6	0.2751346771567486	1.0385550400048702
7	0.17673028896337267	0.7782909201342502
8	0.10970340326942928	0.5521321342141257
9	0.10357062205394352	0.5864243975466702
>10	0.730375237205148	8.669536500871503
>50	0.03543936494488702	1.443523613498088
>100	0.001972783873273467	0.14902247783553046
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.957510430969924E-4	0.0
2	0.0	0.0	0.0	0.0011494181733623223	0.0
3	0.0	0.0	0.0	0.0012378349559286547	0.0
4	0.0	0.0	0.0	0.0019451692164593145	0.0
5	0.0	0.0	0.0	0.0021220027815919795	0.0
6	0.0	0.0	0.0	0.0024756699118573095	0.0
7	0.0	0.0	0.0	0.0024756699118573095	0.0
8	0.0	0.0	0.0	0.0024756699118573095	0.0
9	0.0	0.0	0.0	0.0026525034769899745	0.0
10	0.0	0.0	0.0	0.002917753824688972	0.0
11	0.0	0.0	0.0	0.0030061706072553045	0.0
12	0.0	0.0	0.0	0.0031830041723879695	0.0
13	0.0	0.0	0.0	0.003271420954954302	0.0
14	0.0	0.0	0.0	0.0036250880852196315	0.0
15	0.0	0.0	0.0	0.003978755215484962	0.0
16	0.0	0.0	0.0	0.004244005563183959	0.0
17	0.0	0.0	0.0	0.004509255910882957	0.0
18	0.0	0.0	0.0	0.005128173388847284	0.0
19	0.0	0.0	0.0	0.005216590171413616	0.0
20	0.0	0.0	0.0	0.005747090866811611	0.0
21	0.0	0.0	0.0	0.006100757997076941	0.0
22	0.0	0.0	0.0	0.007161759387872931	0.0
23	0.0	0.0	0.0	0.007515426518138261	0.0
24	0.0	0.0	0.0	0.00822276077866892	0.0
25	0.0	0.0	0.0	0.008488011126367918	0.0
26	0.0	0.0	0.0	0.009460595734597576	0.0
27	0.0	0.0	0.0	0.013704601297781534	0.0
28	0.0	0.0	0.0	0.021750528511317792	0.0
29	0.0	0.0	0.0	0.03660454798246165	0.0
30	0.0	0.0	0.0	0.052431152061835164	0.0
31	0.0	0.0	0.0	0.069141923966872	0.0
32	0.0	0.0	0.0	0.0857642790893425	0.0
33	0.0	0.0	0.0	0.10910630968685428	0.0
34	0.0	0.0	0.0	0.14199735280152997	0.0
35	0.0	0.0	0.0	0.1932790866900028	0.0
36	0.0	0.0	0.0	0.28054645108297294	0.0
37	0.0	0.0	0.0	0.4396082429198051	0.0
38	0.0	0.0	0.0	0.6816933935864234	0.0
39	0.0	0.0	0.0	0.9934509689153117	0.0
40	0.0	0.0	0.0	1.3594964487399281	0.0
41	0.0	0.0	0.0	1.7323500208221523	0.0
42	0.0	0.0	0.0	2.1313749605440107	0.0
43	0.0	0.0	0.0	2.5594890217301924	0.0
44	0.0	0.0	0.0	3.021201460291581	0.0
45	0.0	0.0	0.0	3.5673519262038167	0.0
46	0.0	0.0	0.0	4.1640767917439945	0.0
47	0.0	0.0	0.0	4.848422688807408	0.0
48	0.0	0.0	0.0	5.5648638779424	0.0
49	0.0	0.0	0.0	6.296778004026501	0.0
50	0.0	0.0	0.0	7.0221492882006915	0.0
51	0.0	0.0	0.0	7.715955780998703	0.0
52	0.0	0.0	0.0	8.382087820853451	0.0
53	0.0	0.0	0.0	9.001005298817779	0.0
54	0.0	0.0	0.0	9.605334007658662	0.0
55	0.0	0.0	0.0	10.226550321969714	0.0
56	0.0	0.0	0.0	10.84449521532581	0.0
57	0.0	0.0	0.0	11.483483302932695	0.0
58	0.0	0.0	0.0	12.146520755397624	0.0
59	0.0	0.0	0.0	12.803369033082907	0.0
60	0.0	0.0	0.0	13.499828029357909	0.0
61	0.0	0.0	0.0	14.158002558781687	0.0
62	0.0	0.0	0.0	14.765072187882126	0.0
63	0.0	0.0	0.0	15.36091288559664	0.0
64	0.0	0.0	0.0	15.904852931944719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	75	1.4918078E-6	32.666668	70
TATACCG	65	7.959256E-4	26.923077	6
AAACGGT	70	0.0012265425	25.000002	20
AACCGTA	90	1.9288462E-4	23.333332	10
ATCCTCG	110	2.9234057E-5	22.272728	34
TATCGGC	95	2.7813684E-4	22.105263	15
GGTATAC	150	6.385162E-7	21.000002	1
CGTTCGG	85	0.0037907283	20.588236	24
GTATCGG	105	5.466485E-4	20.000002	14
TCTACGC	90	0.005276814	19.444443	3
TATCGGT	90	0.005276814	19.444443	24
TATGCGG	145	1.1171436E-5	19.310345	43
CGTATCG	110	7.4770185E-4	19.09091	13
ATACCGC	110	7.4770185E-4	19.09091	7
CATATCG	130	1.0576176E-4	18.846153	58
CGCCATA	150	1.4964369E-5	18.666668	55
CAACCGT	115	0.0010080044	18.260868	9
CGATAAA	1720	0.0	17.70349	70
GTAGCGA	100	0.009690278	17.500002	49
AGCGTTC	120	0.0013410505	17.5	27
>>END_MODULE