FastQCFastQC Report
Wed 25 May 2016
SRR1781181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012312
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT37750.3729087475007705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6250.052.63999643
CGTATGC6400.051.4062541
ATCTCGT5800.050.68965537
TCTCGTA5850.049.6581238
TCGTATG6650.048.94736540
ATGCCGT6750.048.7407444
CTCGTAT6650.047.89473339
GCCGTCT7000.047.00000446
CCGTCTT7100.046.33802847
TGCCGTC7250.045.8620745
GTATGCC7250.045.3793142
CGTCTTC7300.045.06849348
CATCTCG6850.042.9197136
GCATCTC8800.033.80681635
AGCATCT8950.033.24022334
GTCTTCT10500.031.33333449
CTGCTTG10400.030.96153854
CAGCATC10650.028.5915533
CCAGCAT10650.028.5915532
ACCAGCA11900.028.52941131
Produced by FastQC (version 0.11.5)