##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781181_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012312
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.37693616197378	34.0	34.0	34.0	33.0	34.0
2	33.371738159776825	34.0	34.0	34.0	33.0	34.0
3	33.449324911687306	34.0	34.0	34.0	34.0	34.0
4	33.50980330174887	34.0	34.0	34.0	34.0	34.0
5	33.5055447332443	34.0	34.0	34.0	34.0	34.0
6	36.992176325085545	38.0	37.0	38.0	35.0	38.0
7	36.8901524431203	38.0	37.0	38.0	35.0	38.0
8	36.72869530342424	38.0	37.0	38.0	34.0	38.0
9	36.674302981689436	38.0	37.0	38.0	34.0	38.0
10-11	36.616785635258694	38.0	37.0	38.0	34.0	38.0
12-13	36.56666472391911	38.0	37.0	38.0	34.0	38.0
14-15	36.57537745280111	38.0	37.0	38.0	34.0	38.0
16-17	36.53902403606793	38.0	37.0	38.0	34.0	38.0
18-19	36.546756335991276	38.0	37.0	38.0	34.0	38.0
20-21	36.49057652186282	38.0	37.0	38.0	34.0	38.0
22-23	36.532840171804736	38.0	37.0	38.0	34.0	38.0
24-25	36.538235247631164	38.0	37.0	38.0	34.0	38.0
26-27	36.36213094381969	38.0	37.0	38.0	34.0	38.0
28-29	36.35032084969851	38.0	37.0	38.0	34.0	38.0
30-31	36.381588383818425	38.0	37.0	38.0	34.0	38.0
32-33	36.34604203052024	38.0	37.0	38.0	34.0	38.0
34-35	36.35816477528667	38.0	37.0	38.0	34.0	38.0
36-37	36.322410482143844	38.0	37.0	38.0	34.0	38.0
38-39	36.31910171962794	38.0	37.0	38.0	34.0	38.0
40-41	36.31246048649033	38.0	37.0	38.0	34.0	38.0
42-43	36.30822068690286	38.0	37.0	38.0	34.0	38.0
44-45	36.28055085783829	38.0	37.0	38.0	34.0	38.0
46-47	36.299434858027965	38.0	37.0	38.0	34.0	38.0
48-49	36.29323568227977	38.0	37.0	38.0	34.0	38.0
50-51	36.217251203186365	38.0	37.0	38.0	34.0	38.0
52-53	36.24818534206845	38.0	37.0	38.0	34.0	38.0
54-55	36.260286848323446	38.0	37.0	38.0	34.0	38.0
56-57	36.205195631386374	38.0	37.0	38.0	34.0	38.0
58-59	36.219388390140594	38.0	37.0	38.0	34.0	38.0
60-61	36.222985601277074	38.0	37.0	38.0	34.0	38.0
62-63	36.19126464963371	38.0	37.0	38.0	34.0	38.0
64-65	36.15179509874426	38.0	37.0	38.0	34.0	38.0
66-67	36.14551195678803	38.0	37.0	38.0	34.0	38.0
68-69	36.11791917906733	38.0	37.0	38.0	33.5	38.0
70-71	36.08048309216922	38.0	37.0	38.0	33.0	38.0
72-73	36.05081190383992	38.0	37.0	38.0	33.0	38.0
74-75	36.06354019314203	38.0	37.0	38.0	33.0	38.0
76	35.273747619311045	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	27.0
22	91.0
23	211.0
24	459.0
25	856.0
26	1702.0
27	3085.0
28	5511.0
29	9310.0
30	15001.0
31	23154.0
32	34796.0
33	50217.0
34	72314.0
35	105613.0
36	171192.0
37	518763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.92940516362544	18.217506065323736	9.999486324374303	24.85360244667652
2	23.23226436118509	22.770845352025855	30.349141371434897	23.64774891535416
3	20.267862082045852	23.094559779988778	30.398533258521088	26.239044879444286
4	14.382818735725744	17.95069109128411	36.8992958692577	30.76719430373245
5	13.74102055492773	33.7387090146121	36.088281083302384	16.431989347157796
6	29.180430539201353	37.597400801333976	19.44805554018919	13.77411311927548
7	27.404100712033447	31.415117078529146	22.196911624084276	18.98387058535313
8	24.85873920293348	36.239321474011966	20.615976102229354	18.2859632208252
9	24.56979666347924	18.172954583171986	21.852748954867668	35.4044997984811
10-11	24.05829428081461	25.97894720204838	28.16285888145157	21.799899635685442
12-13	24.8737049447206	24.15954764934131	27.484658879871027	23.482088526067063
14-15	22.616347529220242	24.888967037830234	24.91731798101771	27.577367451931817
16-17	22.809222848291828	28.56782296367128	25.58583717272936	23.037117015307533
18-19	22.208172974339927	27.883547759979137	27.3480903120777	22.560188953603237
20-21	22.13082527916295	28.04930693304041	27.225104513233074	22.594763274563572
22-23	22.206493650178995	27.949930456222983	27.385578754376123	22.4579971392219
24-25	22.5638933451347	27.794642363223986	27.1305684413501	22.510895850291217
26-27	22.197306759180965	27.68948703561748	27.391703348374808	22.72150285682675
28-29	21.91542725958005	27.80501465951209	27.71442993859601	22.565128142311856
30-31	22.388107618994933	28.041848758090392	27.434130979381848	22.135912643532823
32-33	21.771400516836707	28.01690585511186	27.557215562000646	22.654478066050782
34-35	22.264380941844017	27.545954211744995	27.955363563802464	22.234301282608524
36-37	22.294016074095733	27.741546084606327	27.246787551663914	22.717650289634026
38-39	21.85561368431867	27.68820284655324	27.825462900765775	22.63072056836232
40-41	21.806369972893734	27.087548107698023	28.42685851792728	22.679223401480968
42-43	21.96788144366559	26.996617643572336	28.334594472850267	22.700906439911805
44-45	21.661009649199062	26.99642007602399	28.52539533266424	22.817174942112707
46-47	21.76107761243569	26.698883348216757	28.45190020467998	23.088138834667575
48-49	21.372659812389855	26.485362220343134	28.903984147179923	23.237993820087087
50-51	21.08974308316013	26.523838500383278	29.075127035933583	23.311291380523
52-53	20.66556555686389	26.49346248982527	29.6467887370692	23.194183216241633
54-55	20.37598092287753	26.637341056907353	29.501280237713274	23.48539778250184
56-57	20.40388733908123	26.51880052790049	29.190358308505676	23.8869538245126
58-59	20.043128995803666	26.320788452571936	29.56637874489288	24.06970380673152
60-61	20.305943226989307	26.815793944949778	28.635292281431024	24.242970546629895
62-63	19.78061111594054	27.05499885410822	28.551918776029524	24.612471253921715
64-65	19.754729767107374	26.790159555552044	28.31706035293467	25.138050324405913
66-67	19.76826314416899	26.8311054299465	27.86245742419333	25.53817400169118
68-69	20.130503244059145	26.926283596361593	27.479275164178635	25.463937995400627
70-71	20.55398928393618	27.024820411098556	26.851899414409786	25.56929089055548
72-73	20.29700329542671	26.946534270066934	26.584294170176783	26.172168264329578
74-75	20.357212005784778	27.012620614988265	26.205507788112758	26.424659591114203
76	20.519365571088755	27.06369182623539	25.869593564039544	26.54734903863631
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	1.0
8	0.0
9	0.0
10	3.5
11	13.0
12	19.0
13	20.0
14	45.5
15	115.5
16	261.0
17	361.0
18	527.0
19	911.5
20	1460.5
21	1791.0
22	2378.0
23	3701.0
24	5375.5
25	6314.0
26	7593.0
27	10143.0
28	13181.0
29	14948.0
30	16710.0
31	20376.0
32	24415.5
33	26551.0
34	28750.5
35	33139.5
36	37524.5
37	39720.0
38	42129.0
39	46237.5
40	49445.5
41	52166.0
42	53378.0
43	54064.0
44	54493.0
45	54010.5
46	53785.0
47	52126.0
48	48221.0
49	44003.0
50	42031.0
51	40261.0
52	36468.5
53	32904.0
54	31362.0
55	29903.5
56	26881.0
57	23312.0
58	21307.0
59	19538.0
60	16292.0
61	13376.5
62	11938.0
63	10884.5
64	9133.0
65	7634.5
66	6437.5
67	6041.0
68	5559.0
69	4792.0
70	4248.5
71	3990.0
72	3701.5
73	3250.0
74	2887.5
75	2688.0
76	2508.5
77	2099.5
78	1708.5
79	1547.0
80	1386.5
81	1103.0
82	864.0
83	748.0
84	635.5
85	418.0
86	258.0
87	203.0
88	155.5
89	87.5
90	44.0
91	17.5
92	14.0
93	12.5
94	6.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1012312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.7028600386389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.80410845723046	38.62889368732214
2	22.143415344114256	25.997636234459765
3	7.415185784614797	13.058778398242415
4	2.20773293759915	5.18400950554304
5	0.7351511304280479	2.157773695838243
6	0.32694483496580656	1.1515558132412183
7	0.19352612553229737	0.7952375942659761
8	0.1362694108781258	0.6399523323461117
9	0.1283701234912377	0.6782124053204026
>10	0.8676474720440651	9.58172785177826
>50	0.03777291561234706	1.429872780455011
>100	0.003706965280030945	0.322952114408137
>500	0.0	0.0
>1k	1.6849820938176805E-4	0.37339758677924484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	3775	0.3729087475007705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.902701933791164E-4	0.0
2	0.0	0.0	0.0	0.001086621515896285	0.0
3	0.0	0.0	0.0	0.0011854052900686744	0.0
4	0.0	0.0	0.0	0.0018768917092754013	0.0
5	0.0	0.0	0.0	0.0020744592576201802	0.0
6	0.0	0.0	0.0	0.00217324303179257	0.0
7	0.0	0.0	0.0	0.00217324303179257	0.0
8	0.0	0.0	0.0	0.00217324303179257	0.0
9	0.0	0.0	0.0	0.0022720268059649596	9.878377417238954E-5
10	0.0	0.0	0.0	0.002568378128482128	9.878377417238954E-5
11	0.0	0.0	0.0	0.0026671619026545175	9.878377417238954E-5
12	0.0	0.0	0.0	0.002765945676826907	9.878377417238954E-5
13	0.0	0.0	0.0	0.002864729450999297	9.878377417238954E-5
14	0.0	0.0	0.0	0.0031610807735164654	9.878377417238954E-5
15	0.0	0.0	0.0	0.003457432096033634	9.878377417238954E-5
16	0.0	0.0	0.0	0.003654999644378413	9.878377417238954E-5
17	0.0	0.0	0.0	0.0037537834185508026	9.878377417238954E-5
18	0.0	0.0	0.0	0.0041489185152403605	9.878377417238954E-5
19	0.0	0.0	0.0	0.0041489185152403605	9.878377417238954E-5
20	0.0	0.0	0.0	0.004741621160274698	9.878377417238954E-5
21	0.0	0.0	0.0	0.005136756256964256	9.878377417238954E-5
22	0.0	0.0	0.0	0.0061245939986881515	9.878377417238954E-5
23	0.0	0.0	0.0	0.006519729095377709	9.878377417238954E-5
24	0.0	0.0	0.0	0.006914864192067268	9.878377417238954E-5
25	0.0	0.0	0.0	0.007211215514584437	9.878377417238954E-5
26	0.0	0.0	0.0	0.008495404578825501	9.878377417238954E-5
27	0.0	0.0	0.0	0.013533377061617367	9.878377417238954E-5
28	0.0	0.0	0.0	0.022621484285477205	9.878377417238954E-5
29	0.0	0.0	0.0	0.03832810437888714	9.878377417238954E-5
30	0.0	0.0	0.0	0.05610918372991726	9.878377417238954E-5
31	0.0	0.0	0.0	0.07448296572598172	9.878377417238954E-5
32	0.0	0.0	0.0	0.09186890998032227	9.878377417238954E-5
33	0.0	0.0	0.0	0.11765147503931594	9.878377417238954E-5
34	0.0	0.0	0.0	0.1528184986446866	9.878377417238954E-5
35	0.0	0.0	0.0	0.2076434933103628	9.878377417238954E-5
36	0.0	0.0	0.0	0.30198199764499484	9.878377417238954E-5
37	0.0	0.0	0.0	0.47426089980164216	9.878377417238954E-5
38	0.0	0.0	0.0	0.7355439824876125	9.878377417238954E-5
39	0.0	0.0	0.0	1.0676550312551862	9.878377417238954E-5
40	0.0	0.0	0.0	1.4628889117189168	9.878377417238954E-5
41	0.0	0.0	0.0	1.8587154948276816	9.878377417238954E-5
42	0.0	0.0	0.0	2.2842759939623356	9.878377417238954E-5
43	0.0	0.0	0.0	2.7376935174136037	9.878377417238954E-5
44	0.0	0.0	0.0	3.2283525237278625	9.878377417238954E-5
45	0.0	0.0	0.0	3.8042619271528935	9.878377417238954E-5
46	0.0	0.0	0.0	4.4346999739210835	9.878377417238954E-5
47	0.0	0.0	0.0	5.15345071479939	9.878377417238954E-5
48	0.0	0.0	0.0	5.909245370992342	9.878377417238954E-5
49	0.0	0.0	0.0	6.678375836698567	9.878377417238954E-5
50	0.0	0.0	0.0	7.439603600471001	9.878377417238954E-5
51	0.0	0.0	0.0	8.165664340638065	9.878377417238954E-5
52	0.0	0.0	0.0	8.857743462489825	9.878377417238954E-5
53	0.0	0.0	0.0	9.508530966737528	9.878377417238954E-5
54	0.0	0.0	0.0	10.140154418795786	9.878377417238954E-5
55	0.0	0.0	0.0	10.791238274366005	9.878377417238954E-5
56	0.0	0.0	0.0	11.437778076324296	9.878377417238954E-5
57	0.0	0.0	0.0	12.131734089885331	9.878377417238954E-5
58	0.0	0.0	0.0	12.826282806091402	9.878377417238954E-5
59	0.0	0.0	0.0	13.53841503410016	9.878377417238954E-5
60	0.0	0.0	0.0	14.264475774267222	9.878377417238954E-5
61	0.0	0.0	0.0	14.945293545863331	9.878377417238954E-5
62	0.0	0.0	0.0	15.576423079050727	9.878377417238954E-5
63	0.0	0.0	0.0	16.195599775563267	9.878377417238954E-5
64	0.0	0.0	0.0	16.769039584633987	9.878377417238954E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	625	0.0	52.639996	43
CGTATGC	640	0.0	51.40625	41
ATCTCGT	580	0.0	50.689655	37
TCTCGTA	585	0.0	49.65812	38
TCGTATG	665	0.0	48.947365	40
ATGCCGT	675	0.0	48.74074	44
CTCGTAT	665	0.0	47.894733	39
GCCGTCT	700	0.0	47.000004	46
CCGTCTT	710	0.0	46.338028	47
TGCCGTC	725	0.0	45.86207	45
GTATGCC	725	0.0	45.37931	42
CGTCTTC	730	0.0	45.068493	48
CATCTCG	685	0.0	42.91971	36
GCATCTC	880	0.0	33.806816	35
AGCATCT	895	0.0	33.240223	34
GTCTTCT	1050	0.0	31.333334	49
CTGCTTG	1040	0.0	30.961538	54
CAGCATC	1065	0.0	28.59155	33
CCAGCAT	1065	0.0	28.59155	32
ACCAGCA	1190	0.0	28.529411	31
>>END_MODULE