FastQCFastQC Report
Wed 25 May 2016
SRR1781180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199528
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCCG150.00222220370.032
GCGAAGT407.452927E-752.532
CACGCGA200.006943661352.536
CGCGATA200.006943661352.538
CGATTAA200.006943661352.542
CGGATCC200.006943661352.531
TTGCGAG200.006943661352.548
GACGAAT200.006943661352.523
GATAGCA200.006943661352.541
ACGGCTA352.0812182E-550.050
CCGCATG404.5810983E-543.7521
TACCAAG350.001253276140.042
TTTCGTC350.001253276140.018
GCTTGCT459.174581E-538.8888935
ATTTTGA459.174581E-538.8888930
GTTGTCA556.6714274E-638.18181658
CGAAGTT556.6714274E-638.18181633
GTCAAAA654.843241E-737.69230761
CTCAACG501.7054306E-435.00000446
CAACGGC501.7054306E-435.00000448