##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781180_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199528
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477060863638187	34.0	33.0	34.0	23.0	34.0
2	31.523675875065155	34.0	33.0	34.0	23.0	34.0
3	31.541543041578123	34.0	33.0	34.0	23.0	34.0
4	31.605228338879755	34.0	34.0	34.0	23.0	34.0
5	31.67782466621226	34.0	34.0	34.0	23.0	34.0
6	34.613232228058216	38.0	35.0	38.0	26.0	38.0
7	34.55545587586705	38.0	35.0	38.0	26.0	38.0
8	34.28911731686781	37.0	34.0	38.0	24.0	38.0
9	34.60289282707189	38.0	35.0	38.0	26.0	38.0
10-11	34.45612645844192	38.0	34.5	38.0	25.5	38.0
12-13	34.406133475001	38.0	34.0	38.0	25.0	38.0
14-15	34.281361513171085	37.5	34.0	38.0	24.0	38.0
16-17	34.26954612886412	37.5	34.0	38.0	24.5	38.0
18-19	34.337461408925066	37.5	34.0	38.0	25.0	38.0
20-21	34.25348071448619	37.0	34.0	38.0	24.5	38.0
22-23	34.31676255964075	37.5	34.0	38.0	24.5	38.0
24-25	34.31434435267231	37.5	34.0	38.0	24.5	38.0
26-27	34.23733009903372	37.0	34.0	38.0	24.0	38.0
28-29	34.329054067599536	37.0	34.0	38.0	24.5	38.0
30-31	34.41199731366024	38.0	34.0	38.0	25.0	38.0
32-33	34.33963153041177	38.0	34.0	38.0	24.5	38.0
34-35	34.380109558558196	38.0	34.0	38.0	24.5	38.0
36-37	34.22578284752015	37.0	34.0	38.0	24.0	38.0
38-39	34.2498371155928	37.0	34.0	38.0	24.0	38.0
40-41	34.21874122930115	37.0	34.0	38.0	24.0	38.0
42-43	34.276046469668415	37.0	34.0	38.0	24.0	38.0
44-45	34.30862585702258	37.0	34.0	38.0	24.5	38.0
46-47	34.29312427328495	37.0	34.0	38.0	24.5	38.0
48-49	34.26448167675714	37.0	34.0	38.0	24.0	38.0
50-51	34.12592718816407	37.0	34.0	38.0	24.0	38.0
52-53	34.215295597610364	37.0	34.0	38.0	24.0	38.0
54-55	34.189356882242095	37.0	34.0	38.0	24.0	38.0
56-57	34.17726835331382	37.0	34.0	38.0	24.0	38.0
58-59	34.15727867767932	37.0	34.0	38.0	24.0	38.0
60-61	34.14050659556553	37.0	34.0	38.0	24.0	38.0
62-63	34.10969888937893	37.0	34.0	38.0	24.0	38.0
64-65	34.11583336674552	37.0	34.0	38.0	24.0	38.0
66-67	34.043480113066835	37.0	34.0	38.0	24.0	38.0
68-69	33.80671133875947	37.0	34.0	38.0	23.0	38.0
70-71	33.96008329657993	37.0	34.0	38.0	23.5	38.0
72-73	33.92287298023335	37.0	34.0	38.0	23.5	38.0
74-75	33.90541427769536	37.0	34.0	38.0	23.5	38.0
76	32.4331121446614	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	26.0
14	101.0
15	314.0
16	748.0
17	1222.0
18	1627.0
19	1675.0
20	1515.0
21	1439.0
22	1450.0
23	1619.0
24	1880.0
25	2314.0
26	2743.0
27	3268.0
28	4105.0
29	4832.0
30	6198.0
31	7518.0
32	9194.0
33	11680.0
34	15323.0
35	20218.0
36	31506.0
37	67008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61966240327172	18.78382983841867	10.153963353514293	25.442544404795314
2	23.68138807585903	19.454913596086765	30.901928551381257	25.96176977667295
3	19.535102842708792	21.528807986848964	29.043542760915763	29.89254640952648
4	14.15139729762239	15.98322040014434	36.549256244737585	33.31612605749569
5	14.059680846798445	34.11150314742793	35.04320195661762	16.78561404915601
6	31.030732528767892	36.22849925824947	17.78296780401748	14.957800408965158
7	30.260414578405037	28.40804298143619	22.03199550940219	19.299546930756588
8	26.41032837496492	33.01792229662002	19.532596928751854	21.039152399663205
9	25.043602902850726	16.16164147387835	21.321819494005855	37.472936129265065
10-11	25.948739024096867	23.917445170602623	26.961378854095663	23.172436951204844
12-13	25.77708391804659	22.39635539874103	26.19782687141654	25.62873381179584
14-15	23.65958662443366	23.638035764403995	23.80117076300068	28.90120684816166
16-17	24.165781243735214	26.403061224489793	25.138577041818692	24.292580489956297
18-19	23.628262699971934	26.01740106651698	26.359458321639067	23.994877911872017
20-21	23.467132432540797	26.21060703259693	26.16124052764524	24.161020007217033
22-23	24.016178180505996	25.69463934886332	26.563690309129544	23.725492161501144
24-25	23.390702056854177	26.074535904735175	26.30608235435628	24.22867968405437
26-27	22.710847600336795	26.325377891824708	26.803255683412853	24.160518824425644
28-29	23.370404153802976	26.082554829397374	27.250310733330664	23.296730283468985
30-31	23.591676356200633	26.012389238603102	26.665430415781245	23.73050398941502
32-33	23.085481736899084	26.167004129746203	26.576971653101317	24.1705424802534
34-35	24.172046028627562	25.511707630006814	26.67044224369512	23.645804097670503
36-37	23.8450242572471	25.54854456517381	26.300318752255325	24.306112425323764
38-39	23.078465177819655	26.091074936850966	27.08717573473397	23.743284150595407
40-41	23.526522593320237	24.936850968285153	27.566557074696284	23.97006936369833
42-43	22.968204963714367	25.097981235716286	27.36483100116274	24.568982799406598
44-45	22.43043582855539	24.979952688344493	27.855739545326973	24.733871937773145
46-47	22.57477647247504	24.333677478850085	28.29427448779119	24.797271560883686
48-49	21.84079427448779	24.254240006415138	28.98741028828034	24.917555430816726
50-51	21.73354115713083	24.48603704743194	28.64785894711519	25.13256284832204
52-53	21.302523956537428	24.4509542520348	29.164077222244494	25.08244456918327
54-55	20.528447135239166	25.19546128864119	29.007457599935847	25.268633976183796
56-57	20.215709073413255	25.207740267030193	29.017731847159293	25.558818812397256
58-59	20.730173208772705	25.026312096547855	28.36243534741991	25.881079347259533
60-61	20.439487189767853	24.76143699129947	28.563159055370672	26.235916763562006
62-63	20.02525961268594	24.50508199350467	28.996932761316707	26.472725632492683
64-65	21.156429172847922	24.624363497854937	27.735956858185318	26.483250471111823
66-67	21.167204602862753	24.32866565093621	27.34829196904695	27.155837777154083
68-69	20.888045788059824	24.319894952086926	27.216230303516298	27.575828956336956
70-71	21.539332825468104	24.385299306363017	27.169620303917245	26.905747564251637
72-73	21.46265185838579	24.507838498857303	26.621326330139127	27.40818331261778
74-75	21.583938093901608	25.10625075177419	26.39855057936731	26.9112605749569
76	21.789423038370554	25.306222685537872	26.20985525840985	26.69449901768173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	3.0
15	7.5
16	21.5
17	32.0
18	37.5
19	66.0
20	104.5
21	120.0
22	139.0
23	207.5
24	368.5
25	480.0
26	599.0
27	799.5
28	1063.5
29	1246.0
30	1486.5
31	1903.5
32	2521.0
33	2962.0
34	3427.0
35	4444.5
36	5682.0
37	6367.0
38	6862.0
39	8259.5
40	9995.5
41	11500.5
42	12172.0
43	12370.0
44	12770.0
45	12867.0
46	12762.0
47	12570.0
48	11679.5
49	10425.5
50	9870.0
51	9219.0
52	7690.5
53	6263.0
54	5713.0
55	5259.0
56	4317.5
57	3546.5
58	3263.0
59	2993.5
60	2600.5
61	2299.5
62	2122.0
63	2069.5
64	1949.5
65	1859.5
66	1787.0
67	1737.0
68	1716.0
69	1693.5
70	1670.5
71	1649.0
72	1635.0
73	1589.5
74	1452.5
75	1347.0
76	1301.0
77	1132.0
78	926.5
79	844.0
80	727.5
81	527.5
82	382.0
83	320.0
84	266.5
85	170.5
86	121.5
87	115.0
88	82.0
89	37.5
90	21.0
91	12.5
92	9.0
93	9.5
94	8.0
95	3.5
96	1.0
97	1.0
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	199528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.536405917966405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28663786561985	35.312838298384186
2	13.80932627808861	13.681287839300749
3	5.624298100952053	8.358225411972255
4	2.5131779965398273	4.979752215227938
5	1.4326328675927518	3.548374163024738
6	0.9368771436376329	2.784571588949922
7	0.6991167454142595	2.424221161942184
8	0.5746719412377704	2.277374604065595
9	0.4269569704266535	1.9034922416903892
>10	2.640658039842572	22.932620985525844
>50	0.05159906514634911	1.557174932841506
>100	0.00404698550167444	0.2400665570746963
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010023655827753497	0.0
2	0.0	0.0	0.0	0.0010023655827753497	0.0
3	0.0	0.0	0.0	0.0010023655827753497	0.0
4	0.0	0.0	0.0	0.0020047311655506995	0.0
5	0.0	0.0	0.0	0.0030070967483260495	0.0
6	0.0	0.0	0.0	0.0035082795397137242	0.0
7	0.0	0.0	0.0	0.0035082795397137242	0.0
8	0.0	0.0	0.0	0.0035082795397137242	0.0
9	0.0	0.0	0.0	0.005011827913876749	0.0
10	0.0	0.0	0.0	0.005011827913876749	0.0
11	0.0	0.0	0.0	0.005011827913876749	0.0
12	0.0	0.0	0.0	0.005011827913876749	0.0
13	0.0	0.0	0.0	0.005011827913876749	0.0
14	0.0	0.0	0.0	0.005011827913876749	0.0
15	0.0	0.0	0.0	0.005011827913876749	0.0
16	0.0	0.0	0.0	0.005011827913876749	0.0
17	0.0	0.0	0.0	0.005011827913876749	0.0
18	0.0	0.0	0.0	0.005011827913876749	0.0
19	0.0	0.0	0.0	0.008018924662202798	0.0
20	0.0	0.0	0.0	0.008018924662202798	0.0
21	0.0	0.0	0.0	0.008018924662202798	0.0
22	0.0	0.0	0.0	0.010023655827753499	0.0
23	0.0	0.0	0.0	0.010023655827753499	0.0
24	0.0	0.0	0.0	0.011527204201916523	0.0
25	0.0	0.0	0.0	0.012529569784691873	0.0
26	0.0	0.0	0.0	0.012529569784691873	0.0
27	0.0	0.0	0.0	0.02556032236077142	0.0
28	0.0	0.0	0.0	0.046609999599053764	0.0
29	0.0	0.0	0.0	0.06916322521149913	0.0
30	0.0	0.0	0.0	0.08620344011868009	0.0
31	0.0	0.0	0.0	0.10474720340002405	0.0
32	0.0	0.0	0.0	0.13481817088328454	0.0
33	0.0	0.0	0.0	0.1914518263100918	0.0
34	0.0	0.0	0.0	0.2641233310613047	0.0
35	0.0	0.0	0.0	0.3718776312096548	0.0
36	0.0	0.0	0.0	0.5653341886852973	0.0
37	0.0	0.0	0.0	0.8770698849284311	0.0
38	0.0	0.0	0.0	1.2539593440519625	0.0
39	0.0	0.0	0.0	1.7511326731085362	0.0
40	0.0	0.0	0.0	2.280381700813921	0.0
41	0.0	0.0	0.0	2.8682691151116635	0.0
42	0.0	0.0	0.0	3.561404915600818	0.0
43	0.0	0.0	0.0	4.374323403231626	0.0
44	0.0	0.0	0.0	5.299506836133275	0.0
45	0.0	0.0	0.0	6.333446934766048	0.0
46	0.0	0.0	0.0	7.429533699530893	0.0
47	0.0	0.0	0.0	8.535142937332104	0.0
48	0.0	0.0	0.0	9.631229702096949	0.0
49	0.0	0.0	0.0	10.636602381620625	0.0
50	0.0	0.0	0.0	11.625937211819895	0.0
51	0.0	0.0	0.0	12.534080429814361	0.0
52	0.0	0.0	0.0	13.488833647407883	0.0
53	0.0	0.0	0.0	14.411511166352593	0.0
54	0.0	0.0	0.0	15.353233631370033	0.0
55	0.0	0.0	0.0	16.361112224850647	0.0
56	0.0	0.0	0.0	17.40357243093701	0.0
57	0.0	0.0	0.0	18.318732208010907	0.0
58	0.0	0.0	0.0	19.224369512048433	0.0
59	0.0	0.0	0.0	20.103945310933803	0.0
60	0.0	0.0	0.0	20.983521109819172	0.0
61	0.0	0.0	0.0	21.84254841425765	0.0
62	0.0	0.0	0.0	22.688544966120045	0.0
63	0.0	0.0	0.0	23.524016679363296	0.0
64	0.0	0.0	0.0	24.339942263742433	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCCG	15	0.002222203	70.0	32
GCGAAGT	40	7.452927E-7	52.5	32
CACGCGA	20	0.0069436613	52.5	36
CGCGATA	20	0.0069436613	52.5	38
CGATTAA	20	0.0069436613	52.5	42
CGGATCC	20	0.0069436613	52.5	31
TTGCGAG	20	0.0069436613	52.5	48
GACGAAT	20	0.0069436613	52.5	23
GATAGCA	20	0.0069436613	52.5	41
ACGGCTA	35	2.0812182E-5	50.0	50
CCGCATG	40	4.5810983E-5	43.75	21
TACCAAG	35	0.0012532761	40.0	42
TTTCGTC	35	0.0012532761	40.0	18
GCTTGCT	45	9.174581E-5	38.88889	35
ATTTTGA	45	9.174581E-5	38.88889	30
GTTGTCA	55	6.6714274E-6	38.181816	58
CGAAGTT	55	6.6714274E-6	38.181816	33
GTCAAAA	65	4.843241E-7	37.692307	61
CTCAACG	50	1.7054306E-4	35.000004	46
CAACGGC	50	1.7054306E-4	35.000004	48
>>END_MODULE