FastQCFastQC Report
Wed 25 May 2016
SRR1781180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179805
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT40392.246322404827452No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3950.2196824337476711No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTACCTCGTATGCCGT2410.1340340924890854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC2310.12847251188787853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT150.002221844670.030
TCCAGAT150.002221844670.028
ATCTCGT5750.056.60869237
CTCGTAT6200.056.4516139
GTATCTC5900.055.76271435
TCTCGTA5850.055.64102638
TATGCCG6250.055.4443
TCGTATG6350.055.11811440
CGTATGC6300.054.99999641
CCGTCTT6200.054.75806447
GCCGTCT6350.053.4645746
TGCCGTC6300.053.33333245
ATGCCGT6500.053.30769344
AGTATCT6150.052.92682634
TATCTCG6150.052.92682636
CGTCTTC6350.052.91338748
TCTTCTG6250.052.63999650
GACTTCG200.006942549352.50000429
CGTACAG200.006942549352.5000043
CTGCTTG6500.052.2307754
Produced by FastQC (version 0.11.5)