FastQCFastQC Report
Wed 25 May 2016
SRR1781179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89451
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA150.002218184870.021
TTAGCCC150.002218184870.05
AAGCTAC150.002218184870.019
TAAACGC200.00693120252.513
ATCCGCT200.00693120252.530
TCATCCG200.00693120252.528
AAGGCGT200.00693120252.548
CCGCATT200.00693120252.565
AATTGAC305.8502145E-446.66666451
CAGGAAT350.001249571740.00000413
AAATTGA501.6979288E-435.00000450
CTTTTAA400.002407566235.037
CATAGTG400.002407566235.050
TTTTAAC552.971595E-431.8181838
TTTCCTG500.0071754328.00000243
ACTTTTA500.0071754328.00000236
TCTTACT700.001217730825.00000222
AACAGGG850.003763904320.5882367
AAACTGG950.00716002718.42105370
AAACCCC950.00716002718.4210534
Produced by FastQC (version 0.11.5)