##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781179_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89451
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.866407306793665	34.0	32.0	34.0	21.0	34.0
2	31.07930598875362	34.0	32.0	34.0	21.0	34.0
3	31.015583950989928	34.0	32.0	34.0	23.0	34.0
4	31.155593565192117	34.0	33.0	34.0	23.0	34.0
5	31.15484455176577	34.0	33.0	34.0	23.0	34.0
6	33.87383036522789	37.0	34.0	38.0	22.0	38.0
7	33.941968228415554	37.0	34.0	38.0	23.0	38.0
8	33.6609428625728	37.0	34.0	38.0	21.0	38.0
9	33.94597041955931	37.0	34.0	38.0	23.0	38.0
10-11	33.79687762014958	37.0	34.0	38.0	22.0	38.0
12-13	33.78100300723301	37.0	34.0	38.0	22.0	38.0
14-15	33.640171714122815	37.0	34.0	38.0	21.5	38.0
16-17	33.60478921420666	37.0	34.0	38.0	21.5	38.0
18-19	33.63309521413959	37.0	34.0	38.0	21.5	38.0
20-21	33.58382242792143	37.0	34.0	38.0	21.0	38.0
22-23	33.614034499334835	37.0	34.0	38.0	21.5	38.0
24-25	33.626689472448604	37.0	34.0	38.0	21.5	38.0
26-27	33.55162602989346	37.0	34.0	38.0	21.0	38.0
28-29	33.60957395669138	37.0	34.0	38.0	21.5	38.0
30-31	33.66273155135214	37.0	34.0	38.0	22.0	38.0
32-33	33.648690344434385	37.0	34.0	38.0	21.5	38.0
34-35	33.64628679388716	37.0	34.0	38.0	21.5	38.0
36-37	33.517730377525126	37.0	34.0	38.0	21.0	38.0
38-39	33.536271254653386	37.0	34.0	38.0	21.0	38.0
40-41	33.50355501894892	37.0	34.0	38.0	21.0	38.0
42-43	33.58145241528882	37.0	34.0	38.0	21.0	38.0
44-45	33.62042906172094	37.0	34.0	38.0	21.5	38.0
46-47	33.5807704776917	37.0	34.0	38.0	21.0	38.0
48-49	33.5585460196085	37.0	34.0	38.0	21.5	38.0
50-51	33.421934914087046	37.0	34.0	38.0	21.0	38.0
52-53	33.52717689014097	37.0	34.0	38.0	21.0	38.0
54-55	33.49162670065175	37.0	34.0	38.0	21.0	38.0
56-57	33.44434942035304	37.0	34.0	38.0	21.0	38.0
58-59	33.405266570524645	37.0	34.0	38.0	21.0	38.0
60-61	33.38286324356352	37.0	34.0	38.0	21.0	38.0
62-63	33.33020871762194	37.0	34.0	38.0	21.0	38.0
64-65	33.388475254608664	37.0	34.0	38.0	21.0	38.0
66-67	33.25429564789661	37.0	34.0	38.0	21.0	38.0
68-69	33.04038523884585	37.0	33.5	38.0	20.0	38.0
70-71	33.18006506355435	37.0	34.0	38.0	21.0	38.0
72-73	33.11769013202759	37.0	33.0	38.0	21.0	38.0
74-75	33.138338308124005	37.0	33.0	38.0	21.0	38.0
76	31.75401057562241	36.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	26.0
14	96.0
15	289.0
16	668.0
17	1129.0
18	1401.0
19	1387.0
20	1139.0
21	949.0
22	861.0
23	785.0
24	1031.0
25	1099.0
26	1320.0
27	1568.0
28	1824.0
29	2267.0
30	2767.0
31	3175.0
32	3876.0
33	4991.0
34	6299.0
35	8377.0
36	13092.0
37	29030.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.7680741411499	17.722552011715912	10.137393656862416	26.37198019027177
2	23.306614794692067	19.91481369688433	30.925311064158027	25.853260444265576
3	20.633643000078255	21.07522554247577	28.04440419894691	30.246727258499067
4	15.364836614459312	15.724810231299818	34.538462398408065	34.3718907558328
5	15.345831796178913	32.52506959117282	33.7782696671921	18.350828945456172
6	31.646376228326123	34.271277011995394	17.361460464388323	16.720886295290157
7	30.404355457177672	27.231668734838067	20.808040156063097	21.555935651921164
8	25.985176241741286	32.93311421895787	19.223932655867458	21.857776883433388
9	24.535220399995527	15.84666465439179	20.594515433030374	39.02359951258231
10-11	26.9862829929235	22.98800460587361	25.944371778962783	24.08134062224011
12-13	26.257951280589374	21.344087824619066	25.114308392304167	27.283652502487392
14-15	24.60453209019463	22.358609741646266	22.716347497512604	30.3205106706465
16-17	25.217716962359283	24.572671071312786	23.889615543705492	26.319996422622445
18-19	24.48379559758974	24.453611474438517	24.905255391219775	26.157337536751964
20-21	24.464790779309343	24.814144056522565	24.707381695006205	26.013683469161887
22-23	24.793462342511543	24.2115795239852	24.973449150931796	26.02150898257146
24-25	24.769985802282815	23.924271388805042	24.680551363316226	26.625191445595913
26-27	24.20598987154979	24.688376876725805	24.794021307755084	26.311611943969325
28-29	24.611798638360664	24.297101206246996	25.9052442119149	25.185855943477435
30-31	24.842651283943166	24.178600574616272	24.81582095225319	26.162927189187375
32-33	24.137796111837766	24.781165107153637	24.923701244256634	26.157337536751964
34-35	25.21100937943679	23.74484354562833	25.243429363562175	25.800717711372705
36-37	25.247901085510506	23.64590669752155	24.934321583883914	26.171870633084033
38-39	24.131088528915274	23.892410369923198	25.79233323271959	26.18416786844194
40-41	24.448580787246648	23.524611239673117	26.26968955070374	25.757118422376497
42-43	23.937686554650032	23.330091334920795	26.172988563571113	26.559233546858056
44-45	23.31276341237102	23.441884383629024	26.94380163441437	26.301550569585586
46-47	23.681680473108184	23.46368402812713	26.89852544968754	25.956110049077147
48-49	23.15345831796179	23.393813372684484	27.008082637421605	26.444645671932122
50-51	22.871180869973507	23.818067992532225	26.455266011559402	26.85548512593487
52-53	22.37593766419604	23.890733474192576	27.181361862919363	26.551966998692023
54-55	22.146202949100623	24.09531475332864	27.36470246280086	26.393779834769877
56-57	22.228370839901174	24.336228773294877	27.027087455702002	26.408312931101946
58-59	22.383204212362077	24.107611988686546	26.69618003152564	26.81300376742574
60-61	22.116018825949403	23.44356127935965	26.85548512593487	27.58493476875608
62-63	22.156823288727907	23.53467261405686	27.046651239225945	27.26185285798929
64-65	22.681132687169512	23.581066729270773	26.365272607349276	27.372527976210435
66-67	22.594493074420633	23.73813596270584	25.824753216844975	27.84261774602855
68-69	22.342958714827112	24.298778101977618	25.863321818649315	27.494941364545955
70-71	22.809694693183978	23.989670322299357	26.115415143486377	27.085219841030284
72-73	22.601200657343128	24.209902628254575	25.43906719880158	27.749829515600723
74-75	22.677778895708265	24.689494807212885	25.402734457971405	27.22999183910744
76	23.22612379962214	24.35187980011403	25.21156834468033	27.210428055583503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	5.0
15	10.0
16	11.5
17	12.0
18	21.5
19	34.5
20	51.5
21	65.0
22	93.0
23	144.5
24	196.0
25	224.0
26	293.0
27	406.0
28	513.5
29	577.0
30	722.5
31	904.5
32	1152.5
33	1364.0
34	1520.5
35	1870.0
36	2368.0
37	2673.0
38	2847.5
39	3278.0
40	3798.5
41	4224.0
42	4385.0
43	4597.5
44	4777.0
45	4690.0
46	4636.0
47	4585.0
48	4271.5
49	3874.5
50	3740.0
51	3416.0
52	2866.0
53	2541.5
54	2443.0
55	2296.5
56	2024.0
57	1817.5
58	1737.0
59	1619.5
60	1493.0
61	1499.0
62	1514.0
63	1453.0
64	1379.5
65	1374.0
66	1429.0
67	1477.0
68	1440.5
69	1393.0
70	1383.5
71	1385.0
72	1410.0
73	1348.5
74	1231.0
75	1200.0
76	1140.5
77	984.5
78	789.5
79	691.0
80	582.0
81	428.0
82	330.0
83	277.0
84	218.0
85	125.5
86	75.5
87	59.0
88	46.0
89	25.0
90	14.5
91	7.5
92	3.0
93	2.0
94	0.5
95	0.5
96	1.0
97	1.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	89451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.53593587550726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25023084025854	43.131994052609805
2	15.089566020313944	18.269220019899162
3	6.51708217913204	11.83553006674045
4	2.886426592797784	6.989301405238622
5	1.4090489381348108	4.264904808219025
6	0.8365650969529086	3.0385350638897273
7	0.5060018467220684	2.1441906742238768
8	0.37488457987072943	1.8155191110216768
9	0.25854108956602034	1.4085924137237147
>10	0.8716528162511542	7.102212384433935
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006707582922493879	0.0
2	0.0	0.0	0.0	0.006707582922493879	0.0
3	0.0	0.0	0.0	0.006707582922493879	0.0
4	0.0	0.0	0.0	0.011179304870823132	0.0
5	0.0	0.0	0.0	0.013415165844987758	0.0
6	0.0	0.0	0.0	0.013415165844987758	0.0
7	0.0	0.0	0.0	0.013415165844987758	0.0
8	0.0	0.0	0.0	0.013415165844987758	0.0
9	0.0	0.0	0.0	0.015651026819152387	0.0
10	0.0	0.0	0.0	0.0167689573062347	0.0
11	0.0	0.0	0.0	0.01788688779331701	0.0
12	0.0	0.0	0.0	0.01788688779331701	0.0
13	0.0	0.0	0.0	0.01788688779331701	0.0
14	0.0	0.0	0.0	0.020122748767481637	0.0
15	0.0	0.0	0.0	0.02124067925456395	0.0
16	0.0	0.0	0.0	0.02124067925456395	0.0
17	0.0	0.0	0.0	0.022358609741646263	0.0
18	0.0	0.0	0.0	0.022358609741646263	0.0
19	0.0	0.0	0.0	0.02347654022872858	0.0
20	0.0	0.0	0.0	0.025712401202893205	0.0
21	0.0	0.0	0.0	0.026830331689975517	0.0
22	0.0	0.0	0.0	0.03018412315122246	0.0
23	0.0	0.0	0.0	0.0335379146124694	0.0
24	0.0	0.0	0.0	0.04359928899621022	0.0
25	0.0	0.0	0.0	0.04359928899621022	0.0
26	0.0	0.0	0.0	0.04695308045745716	0.0
27	0.0	0.0	0.0	0.0681937597120211	0.0
28	0.0	0.0	0.0	0.09502409140199662	0.0
29	0.0	0.0	0.0	0.1263261450403014	0.0
30	0.0	0.0	0.0	0.1520385462431946	0.0
31	0.0	0.0	0.0	0.1922840437781579	0.0
32	0.0	0.0	0.0	0.2191143754681334	0.0
33	0.0	0.0	0.0	0.2705391778739198	0.0
34	0.0	0.0	0.0	0.3800963656079865	0.0
35	0.0	0.0	0.0	0.5265452594157696	0.0
36	0.0	0.0	0.0	0.7747258275480431	0.0
37	0.0	0.0	0.0	1.1380532358497948	0.0
38	0.0	0.0	0.0	1.5259751148673575	0.0
39	0.0	0.0	0.0	1.9642038658036243	0.0
40	0.0	0.0	0.0	2.4180836435590436	0.0
41	0.0	0.0	0.0	2.816066896960347	0.0
42	0.0	0.0	0.0	3.297894936892824	0.0
43	0.0	0.0	0.0	3.844562945076075	0.0
44	0.0	0.0	0.0	4.4918446970967345	0.0
45	0.0	0.0	0.0	5.135772657656147	0.0
46	0.0	0.0	0.0	5.840068864518004	0.0
47	0.0	0.0	0.0	6.556662306737767	0.0
48	0.0	0.0	0.0	7.236364042883814	0.0
49	0.0	0.0	0.0	7.898178891236543	0.0
50	0.0	0.0	0.0	8.565583392024683	0.0
51	0.0	0.0	0.0	9.156968619691227	0.0
52	0.0	0.0	0.0	9.781891761970241	0.0
53	0.0	0.0	0.0	10.358743893304714	0.0
54	0.0	0.0	0.0	10.975841522174152	0.0
55	0.0	0.0	0.0	11.597410872991917	0.0
56	0.0	0.0	0.0	12.223451945758013	0.0
57	0.0	0.0	0.0	12.849493018524107	0.0
58	0.0	0.0	0.0	13.469944438854792	0.0
59	0.0	0.0	0.0	14.03002761288303	0.0
60	0.0	0.0	0.0	14.634828006394562	0.0
61	0.0	0.0	0.0	15.204972554806542	0.0
62	0.0	0.0	0.0	15.797475712960168	0.0
63	0.0	0.0	0.0	16.349733373578832	0.0
64	0.0	0.0	0.0	16.889693798839588	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTA	15	0.0022181848	70.0	21
TTAGCCC	15	0.0022181848	70.0	5
AAGCTAC	15	0.0022181848	70.0	19
TAAACGC	20	0.006931202	52.5	13
ATCCGCT	20	0.006931202	52.5	30
TCATCCG	20	0.006931202	52.5	28
AAGGCGT	20	0.006931202	52.5	48
CCGCATT	20	0.006931202	52.5	65
AATTGAC	30	5.8502145E-4	46.666664	51
CAGGAAT	35	0.0012495717	40.000004	13
AAATTGA	50	1.6979288E-4	35.000004	50
CTTTTAA	40	0.0024075662	35.0	37
CATAGTG	40	0.0024075662	35.0	50
TTTTAAC	55	2.971595E-4	31.81818	38
TTTCCTG	50	0.00717543	28.000002	43
ACTTTTA	50	0.00717543	28.000002	36
TCTTACT	70	0.0012177308	25.000002	22
AACAGGG	85	0.0037639043	20.588236	7
AAACTGG	95	0.007160027	18.421053	70
AAACCCC	95	0.007160027	18.421053	4
>>END_MODULE