FastQCFastQC Report
Wed 25 May 2016
SRR1781179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80868
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT39444.877083642479102No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG4310.5329673047435326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2640.3264579314438344No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAACCTCGTATGCCGT2020.24978978087747936No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAACTCGTATGCCGT1250.154572884206361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG150.002217410870.05
CTCGAAA150.002217410870.057
AATCTCG6600.058.8636436
TCTCGTA6700.058.50746538
CGTATGC7550.057.94701841
TCGTATG7550.057.94701840
ATCTCGT6800.057.64705737
CTCGTAT7300.057.53424539
CGTCTTC7250.057.44827748
CCGTCTT7200.057.36110747
GCCGTCT7250.056.9655246
TATGCCG7650.056.7320343
GTATGCC7650.056.7320342
TCTTCTG7350.056.66666850
CTTCTGC7550.056.5562951
TAATCTC6900.056.30434435
TACCACT252.3777202E-455.99999651
AATCGTC252.3777202E-455.99999628
TGAAAAA7450.055.90604459
TTGAAAA7450.055.90604458