##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781179_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80868
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.32036157689073	34.0	34.0	34.0	33.0	34.0
2	33.33471830637582	34.0	34.0	34.0	33.0	34.0
3	33.383019241232624	34.0	34.0	34.0	34.0	34.0
4	33.454889449473214	34.0	34.0	34.0	34.0	34.0
5	33.47352475639313	34.0	34.0	34.0	34.0	34.0
6	36.87958154028787	38.0	37.0	38.0	34.0	38.0
7	36.75552752633922	38.0	37.0	38.0	34.0	38.0
8	36.58963990700895	38.0	37.0	38.0	34.0	38.0
9	36.52739031508137	38.0	37.0	38.0	34.0	38.0
10-11	36.4621172775387	38.0	37.0	38.0	34.0	38.0
12-13	36.41160285898006	38.0	37.0	38.0	34.0	38.0
14-15	36.41792798140179	38.0	37.0	38.0	34.0	38.0
16-17	36.34250877973982	38.0	37.0	38.0	34.0	38.0
18-19	36.30201068407776	38.0	37.0	38.0	34.0	38.0
20-21	36.265624227135575	38.0	37.0	38.0	34.0	38.0
22-23	36.32964213285848	38.0	37.0	38.0	34.0	38.0
24-25	36.29870282435574	38.0	37.0	38.0	34.0	38.0
26-27	36.0150553989217	38.0	36.5	38.0	32.0	38.0
28-29	35.9923640995202	38.0	37.0	38.0	32.0	38.0
30-31	36.07361379037444	38.0	37.0	38.0	32.5	38.0
32-33	36.015624227135575	38.0	37.0	38.0	32.0	38.0
34-35	36.0406464856309	38.0	37.0	38.0	32.0	38.0
36-37	35.985006430231984	38.0	37.0	38.0	32.0	38.0
38-39	35.56104392343077	38.0	36.0	38.0	31.0	38.0
40-41	35.86423554434387	38.0	36.0	38.0	31.5	38.0
42-43	35.995140228520555	38.0	37.0	38.0	32.0	38.0
44-45	35.95302839194737	38.0	36.5	38.0	32.0	38.0
46-47	35.982149923331846	38.0	37.0	38.0	32.0	38.0
48-49	35.91969011228174	38.0	36.5	38.0	31.5	38.0
50-51	35.79164193500519	38.0	36.0	38.0	31.0	38.0
52-53	35.829425730820596	38.0	36.0	38.0	31.0	38.0
54-55	35.81927956670129	38.0	36.0	38.0	31.0	38.0
56-57	35.79158628876688	38.0	36.0	38.0	31.0	38.0
58-59	35.71413909086412	38.0	36.0	38.0	31.0	38.0
60-61	35.693519068111	38.0	36.0	38.0	31.0	38.0
62-63	35.67675718454766	38.0	36.0	38.0	31.0	38.0
64-65	35.62678686254142	38.0	36.0	38.0	31.0	38.0
66-67	35.518078844536774	38.0	36.0	38.0	31.0	38.0
68-69	35.28684399267943	38.0	36.0	38.0	29.0	38.0
70-71	34.80950437750408	38.0	35.5	38.0	25.5	38.0
72-73	34.4447061878617	38.0	35.0	38.0	24.0	38.0
74-75	34.434776425780285	38.0	35.0	38.0	24.0	38.0
76	33.53572488499778	37.0	34.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	6.0
22	13.0
23	40.0
24	72.0
25	149.0
26	264.0
27	490.0
28	772.0
29	1227.0
30	1802.0
31	2722.0
32	3670.0
33	5011.0
34	6854.0
35	9529.0
36	13297.0
37	34948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.442004253845774	16.64069842212	9.517979917890884	31.399317406143346
2	22.406885294554087	18.866547954691594	33.82549339664639	24.90107335410793
3	20.188455260424394	19.69382203096404	31.776475243606868	28.3412474650047
4	14.864965128357325	20.69792748676856	32.574071326111685	31.86303605876243
5	15.202552307464016	31.015976653311572	37.17787010931394	16.603600929910474
6	30.914576841272197	37.938368699609235	16.262304001582827	14.884750457535736
7	28.63679081960726	26.584062917346785	19.410644507097988	25.368501755947964
8	25.646732947519414	36.51506158183707	16.96344660434288	20.87475886630064
9	23.528466142355445	14.613938764406193	18.395409803630606	43.46218528960776
10-11	25.64364148983529	24.878814858782214	26.346020675668992	23.13152297571351
12-13	24.71867735074442	20.1278626898155	26.415887619330263	28.737572340109807
14-15	23.39429687886432	20.778923678092696	20.858683286343176	34.96809615669981
16-17	27.280877479349066	26.104268684770243	21.385467675718456	25.229386160162235
18-19	27.081787604491268	23.41346391650591	22.263441658010585	27.241306820992232
20-21	23.494460107830044	23.286095859919868	22.30363060790424	30.915813424345846
22-23	23.828337537715782	25.918781223722608	22.552802097244893	27.700079141316714
24-25	27.366201711430975	25.71412672503339	22.043329870900727	24.876341692634913
26-27	26.787480832962355	26.34354750952169	22.576915467181085	24.29205619033487
28-29	24.042884700994215	23.017139041400803	26.009051788099125	26.93092446950586
30-31	23.68798535885641	22.43470841371123	26.21123312064104	27.666073106791316
32-33	23.376366424296386	26.187738042241676	26.32314388880645	24.11275164465549
34-35	24.128208933076124	23.127813226492556	29.175322748182225	23.568655092249095
36-37	23.7825839639907	28.45624969085423	23.638522035910373	24.122644309244695
38-39	23.35225305436019	23.804842459316415	25.979992085868332	26.862912400455063
40-41	23.544541722312907	21.8312558737696	27.024286491566503	27.59991591235099
42-43	26.322525597269625	21.9357471434931	26.70957609932235	25.03215115991492
44-45	22.703046940693476	25.706088935054655	27.492333184943362	24.098530939308503
46-47	25.764826631053072	22.437799871395363	24.684671316219024	27.112702181332544
48-49	25.118711975070486	21.988301924123263	24.374907256269477	28.518078844536777
50-51	22.30053915022011	22.239328288074393	27.626502448434486	27.833630113271006
52-53	21.52334668843053	21.983973883365486	31.331305337092548	25.161374091111444
54-55	21.021912252065096	22.142874808329623	28.795073453034576	28.04013948657071
56-57	24.10533214621358	22.335781767819164	25.150863134985407	28.40802295098185
58-59	21.63154770737498	22.42543404065885	30.309269426720086	25.633748825246077
60-61	24.600583667210763	25.40745412276797	24.68096156699807	25.311000643023196
62-63	20.422169461344414	29.287233516347627	24.15046742840184	26.14012959390612
64-65	20.830241875649204	29.74971558589306	23.325048226739874	26.094994311717862
66-67	20.786961468071425	29.48075876737399	23.223030123163674	26.50924964139091
68-69	20.7548103081565	29.874610476331796	23.164910718702085	26.205668496809615
70-71	21.07261215808478	28.020972448929122	23.736212098728792	27.170203294257306
72-73	21.132586437156846	25.62014641143592	24.622223870999655	28.62504328040758
74-75	21.085596280358114	25.158282633427316	25.361700549042887	28.394420537171687
76	21.19503388237622	25.024731661473016	25.660335361329572	28.11989909482119
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	5.5
16	8.5
17	9.0
18	14.5
19	35.0
20	49.0
21	48.0
22	83.5
23	124.0
24	163.5
25	198.0
26	249.5
27	312.5
28	411.0
29	498.0
30	598.0
31	756.5
32	967.0
33	1119.0
34	1218.5
35	1466.5
36	2071.5
37	2528.0
38	3124.5
39	4100.0
40	4565.0
41	4455.5
42	4260.0
43	4165.5
44	4009.0
45	3896.0
46	3845.0
47	3788.5
48	3509.5
49	3154.5
50	3022.0
51	2851.5
52	2447.0
53	2106.5
54	2000.0
55	1935.5
56	1747.0
57	1540.5
58	1458.0
59	1362.5
60	1231.0
61	1194.0
62	1193.0
63	1183.5
64	1221.0
65	1291.0
66	1343.5
67	1373.0
68	1355.5
69	1351.5
70	1287.0
71	1209.0
72	1311.5
73	1278.5
74	1137.5
75	1132.0
76	1042.5
77	875.5
78	748.5
79	699.0
80	605.5
81	433.5
82	285.5
83	216.0
84	174.5
85	105.0
86	59.0
87	41.0
88	28.5
89	16.0
90	9.5
91	2.0
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	80868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.536281347380914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.14378791282857	29.79299599347084
2	21.29608826980204	21.09858040263145
3	8.814498614543547	13.099124499183857
4	4.0415387303727	8.008111984963149
5	1.8372899972540502	4.550625711035267
6	1.1557952020769364	3.4352277786021665
7	0.7114506103497341	2.466983231933521
8	0.48678199655508125	1.9290695948953849
9	0.3594697820714446	1.6026116634515506
>10	1.135824658628523	7.708858881139635
>50	0.004992635862103398	0.16693871494286985
>100	0.009985271724206796	1.2637879012712074
>500	0.0	0.0
>1k	0.002496317931051699	4.877083642479102
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	3944	4.877083642479102	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG	431	0.5329673047435326	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC	264	0.3264579314438344	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAACCTCGTATGCCGT	202	0.24978978087747936	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAACTCGTATGCCGT	125	0.154572884206361	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00618291536825444	0.0
2	0.0	0.0	0.0	0.00618291536825444	0.0
3	0.0	0.0	0.0	0.00618291536825444	0.0
4	0.0	0.0	0.0	0.01236583073650888	0.0
5	0.0	0.0	0.0	0.013602413810159766	0.0
6	0.0	0.0	0.0	0.013602413810159766	0.0
7	0.0	0.0	0.0	0.013602413810159766	0.0
8	0.0	0.0	0.0	0.013602413810159766	0.0
9	0.0	0.0	0.0	0.016075579957461544	0.0
10	0.0	0.0	0.0	0.01731216303111243	0.0
11	0.0	0.0	0.0	0.018548746104763317	0.0
12	0.0	0.0	0.0	0.019785329178414204	0.0
13	0.0	0.0	0.0	0.019785329178414204	0.0
14	0.0	0.0	0.0	0.02225849532571598	0.0
15	0.0	0.0	0.0	0.02349507839936687	0.0
16	0.0	0.0	0.0	0.02349507839936687	0.0
17	0.0	0.0	0.0	0.025968244546668646	0.0
18	0.0	0.0	0.0	0.025968244546668646	0.0
19	0.0	0.0	0.0	0.027204827620319533	0.0
20	0.0	0.0	0.0	0.030914576841272197	0.0
21	0.0	0.0	0.0	0.030914576841272197	0.0
22	0.0	0.0	0.0	0.03338774298857397	0.0
23	0.0	0.0	0.0	0.03338774298857397	0.0
24	0.0	0.0	0.0	0.038334075283177525	0.0
25	0.0	0.0	0.0	0.038334075283177525	0.0
26	0.0	0.0	0.0	0.0408072414304793	0.0
27	0.0	0.0	0.0	0.06306573675619528	0.0
28	0.0	0.0	0.0	0.09398031359746747	0.0
29	0.0	0.0	0.0	0.12736805658604144	0.0
30	0.0	0.0	0.0	0.15580946728001188	0.0
31	0.0	0.0	0.0	0.19785329178414207	0.0
32	0.0	0.0	0.0	0.22876786862541426	0.0
33	0.0	0.0	0.0	0.2881238561606569	0.0
34	0.0	0.0	0.0	0.39941633278923677	0.0
35	0.0	0.0	0.0	0.561408715437503	0.0
36	0.0	0.0	0.0	0.8173814116832369	0.0
37	0.0	0.0	0.0	1.21185141217787	0.0
38	0.0	0.0	0.0	1.6298164910718702	0.0
39	0.0	0.0	0.0	2.093535143690953	0.0
40	0.0	0.0	0.0	2.5819854577830537	0.0
41	0.0	0.0	0.0	2.9912944551614977	0.0
42	0.0	0.0	0.0	3.4908740169164565	0.0
43	0.0	0.0	0.0	4.046099816985705	0.0
44	0.0	0.0	0.0	4.741059504377504	0.0
45	0.0	0.0	0.0	5.395211950338823	0.0
46	0.0	0.0	0.0	6.124795963792848	0.0
47	0.0	0.0	0.0	6.8667458079833805	0.0
48	0.0	0.0	0.0	7.553049413859623	0.0
49	0.0	0.0	0.0	8.222040856704753	0.0
50	0.0	0.0	0.0	8.89968838106544	0.0
51	0.0	0.0	0.0	9.492011673344216	0.0
52	0.0	0.0	0.0	10.128851956274422	0.0
53	0.0	0.0	0.0	10.7236484147005	0.0
54	0.0	0.0	0.0	11.36296186377801	0.0
55	0.0	0.0	0.0	12.005985062076471	0.0
56	0.0	0.0	0.0	12.656427758816838	0.0
57	0.0	0.0	0.0	13.330365533956572	0.0
58	0.0	0.0	0.0	13.984517979917891	0.0
59	0.0	0.0	0.0	14.578077855270317	0.0
60	0.0	0.0	0.0	15.212444972053223	0.0
61	0.0	0.0	0.0	15.80847801355295	0.0
62	0.0	0.0	0.0	16.415640302715538	0.0
63	0.0	0.0	0.0	16.9931245981105	0.0
64	0.0	0.0	0.0	17.552060147400702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAGCG	15	0.0022174108	70.0	5
CTCGAAA	15	0.0022174108	70.0	57
AATCTCG	660	0.0	58.86364	36
TCTCGTA	670	0.0	58.507465	38
CGTATGC	755	0.0	57.947018	41
TCGTATG	755	0.0	57.947018	40
ATCTCGT	680	0.0	57.647057	37
CTCGTAT	730	0.0	57.534245	39
CGTCTTC	725	0.0	57.448277	48
CCGTCTT	720	0.0	57.361107	47
GCCGTCT	725	0.0	56.96552	46
TATGCCG	765	0.0	56.73203	43
GTATGCC	765	0.0	56.73203	42
TCTTCTG	735	0.0	56.666668	50
CTTCTGC	755	0.0	56.55629	51
TAATCTC	690	0.0	56.304344	35
TACCACT	25	2.3777202E-4	55.999996	51
AATCGTC	25	2.3777202E-4	55.999996	28
TGAAAAA	745	0.0	55.906044	59
TTGAAAA	745	0.0	55.906044	58
>>END_MODULE