FastQCFastQC Report
Wed 25 May 2016
SRR1781178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287939
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT150.002223206270.0000131
CGGGTAG305.8720226E-446.66666810
GCGGGTA305.8720226E-446.6666689
ACGGTAC350.00125420240.03
GAAATCG652.09858E-532.30769348
TTACAGC450.004303150331.1111134
GGCTTAT952.812194E-729.4736861
AAATCGC604.9751275E-429.16666849
GGTCGTT500.00720153727.99999811
CCTGGAC1207.369454E-826.2500023
GCTTATG1101.0124768E-625.4545442
TGCGGAG700.001224322425.07
ATGCGGA700.001224322425.06
GACTATC851.3056933E-424.70588338
GGGGACA1001.3940331E-524.4999984
TAGACGG750.001829711623.33333427
TTAGACG750.001829711623.33333426
CTGACAC901.9239906E-423.3333343
CTTATGC901.9239906E-423.3333343
CGGGCTC750.001829711623.33333431
Produced by FastQC (version 0.11.5)