##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781178_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287939
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805517835374854	34.0	34.0	34.0	24.0	34.0
2	31.911529872646636	34.0	34.0	34.0	24.0	34.0
3	31.89740882617499	34.0	34.0	34.0	27.0	34.0
4	32.00838372016295	34.0	34.0	34.0	27.0	34.0
5	31.98821625413716	34.0	34.0	34.0	27.0	34.0
6	35.0353720753354	38.0	36.0	38.0	27.0	38.0
7	34.86495403540333	38.0	35.0	38.0	27.0	38.0
8	34.74348733585933	38.0	35.0	38.0	27.0	38.0
9	34.9893935868361	38.0	36.0	38.0	27.0	38.0
10-11	34.901078006105465	38.0	36.0	38.0	27.0	38.0
12-13	34.87812522791286	38.0	36.0	38.0	27.0	38.0
14-15	34.78229416647276	38.0	35.0	38.0	27.0	38.0
16-17	34.762255199886084	38.0	35.0	38.0	26.5	38.0
18-19	34.82356853361303	38.0	35.0	38.0	27.0	38.0
20-21	34.75101323544223	38.0	35.0	38.0	26.5	38.0
22-23	34.821166983284655	38.0	35.5	38.0	27.0	38.0
24-25	34.8169560219352	38.0	35.5	38.0	27.0	38.0
26-27	34.74626743858942	38.0	35.0	38.0	26.5	38.0
28-29	34.836651165698285	38.0	35.0	38.0	27.0	38.0
30-31	34.900124679185524	38.0	36.0	38.0	27.0	38.0
32-33	34.83924199222752	38.0	35.5	38.0	27.0	38.0
34-35	34.87662838309503	38.0	35.5	38.0	27.0	38.0
36-37	34.76371905160468	38.0	35.0	38.0	26.5	38.0
38-39	34.76139737930603	38.0	35.0	38.0	27.0	38.0
40-41	34.75966263687795	38.0	35.0	38.0	27.0	38.0
42-43	34.82462083983066	38.0	35.0	38.0	27.0	38.0
44-45	34.82902107738097	38.0	35.0	38.0	27.0	38.0
46-47	34.820330000451484	38.0	35.0	38.0	27.0	38.0
48-49	34.800857125988486	38.0	35.0	38.0	27.0	38.0
50-51	34.67304706899725	38.0	35.0	38.0	26.5	38.0
52-53	34.75202212968719	38.0	35.0	38.0	27.0	38.0
54-55	34.730632182510874	38.0	35.0	38.0	27.0	38.0
56-57	34.69063239088835	38.0	35.0	38.0	26.5	38.0
58-59	34.70449991143958	38.0	35.0	38.0	26.5	38.0
60-61	34.676202251171254	38.0	35.0	38.0	26.5	38.0
62-63	34.651130968712124	38.0	35.0	38.0	26.0	38.0
64-65	34.67353328309121	38.0	35.0	38.0	26.0	38.0
66-67	34.571039352084995	38.0	35.0	38.0	25.5	38.0
68-69	34.363259231990114	37.0	34.0	38.0	24.5	38.0
70-71	34.541303887281686	37.5	34.5	38.0	25.0	38.0
72-73	34.50946554652201	38.0	35.0	38.0	25.0	38.0
74-75	34.52586658979853	38.0	35.0	38.0	25.0	38.0
76	33.25598130159513	37.0	33.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	25.0
14	122.0
15	321.0
16	712.0
17	1197.0
18	1488.0
19	1524.0
20	1437.0
21	1489.0
22	1599.0
23	1800.0
24	2244.0
25	2830.0
26	3436.0
27	4246.0
28	5274.0
29	6407.0
30	7885.0
31	10111.0
32	12450.0
33	16105.0
34	21149.0
35	29300.0
36	46459.0
37	108324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93791393315946	20.556090005174706	11.100615060828856	25.405381000836986
2	21.342020358478706	22.008828258763142	32.999350556888785	23.64980082586937
3	19.886503738639085	23.110450477358054	29.615300462945278	27.387745321057583
4	14.167236810574463	17.133837375277402	37.61039664651194	31.088529167636203
5	13.796672211822644	34.44826855688184	36.065277715071595	15.689781516223922
6	29.648640857959496	37.658323464344875	18.352150976422088	14.340884701273534
7	27.85451085125669	29.77297274770003	23.166017802381756	19.206498598661522
8	24.53818343468582	35.15883572562244	20.988125957233997	19.314854882457745
9	24.253400893939343	17.081048416504885	22.69786308905706	35.967687600498714
10-11	24.757848016420144	25.444625424134976	28.10282733495636	21.69469922448852
12-13	24.16032562452464	23.987372325388364	27.68954535509257	24.162756694994425
14-15	22.51744293062072	25.591878835447783	24.85213882106974	27.038539412861752
16-17	22.74387978009231	28.162041265684746	26.331792497716535	22.762286456506413
18-19	22.059880738628667	27.506173182514353	27.815787371630798	22.618158707226183
20-21	22.499904493660118	27.57754246559167	27.364997447376005	22.557555593372207
22-23	22.61676952410059	27.219654162860884	27.729484369953354	22.434091943085164
24-25	22.251761657851144	27.473701026953623	27.35909341909224	22.915443896102992
26-27	21.64434133618579	27.604978832322125	27.804847554516755	22.94583227697533
28-29	22.219115854399714	27.39503853246695	28.396465918128495	21.989379695004846
30-31	22.49903625420662	27.620954438266438	27.644223255620116	22.235786051906828
32-33	21.837264142752456	27.885246527910425	27.644396903510817	22.633092425826305
34-35	22.528209099844066	27.02568946895002	28.02781144617436	22.418289985031553
36-37	22.70307252577803	27.2545573888914	27.40545740590889	22.636912679421684
38-39	22.099646105598755	27.4974907879794	28.037188432272114	22.365674674149734
40-41	22.34883082875192	26.770600717513084	28.402022650630865	22.47854580310413
42-43	21.92512997544619	26.736392083045367	28.226464633134103	23.112013308374344
44-45	21.640000138918314	26.70079426545206	28.413483411417	23.245722184212628
46-47	21.78048128249386	26.53565512139724	28.709032121386823	22.974831474722077
48-49	21.212652679907897	26.487554655673595	29.191252313858147	23.10854035056036
50-51	21.185737256849542	26.36357006171446	29.019340902066066	23.431351779369937
52-53	21.046645296399586	26.232118608455263	29.425156022629796	23.29608007251536
54-55	20.415608861599157	26.712775969910297	29.372367063857276	23.49924810463327
56-57	20.266271675597956	26.773900027436365	29.213131948086225	23.74669634887945
58-59	20.503648342183585	26.52610448740879	29.08289603006192	23.887351140345697
60-61	20.18517811064149	26.607892643928054	29.027155057147517	24.179774188282934
62-63	20.270612872865435	26.17689857921296	29.295093752496186	24.25739479542542
64-65	20.54844949798395	26.1692580720222	28.914457576083823	24.367834853910033
66-67	20.60262763988206	26.014711449300027	28.588346837350965	24.79431407346695
68-69	20.66045238748485	25.85669186876387	28.316935184188317	25.165920559562966
70-71	21.069566817971864	26.041105928686285	28.157179124745173	24.73214812859668
72-73	20.808921334032558	26.195826199299155	27.99099809334616	25.004254373322127
74-75	20.678685416008253	26.553019910467146	27.681557552120417	25.086737121404184
76	21.390641767874445	26.449004823938406	27.39816419449953	24.76218921368762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	4.5
11	8.5
12	10.0
13	12.5
14	26.5
15	64.5
16	120.5
17	150.0
18	194.0
19	315.0
20	500.5
21	609.0
22	720.5
23	980.0
24	1335.5
25	1543.0
26	1868.0
27	2414.0
28	3041.0
29	3447.0
30	3743.5
31	4484.0
32	5707.0
33	6486.0
34	7019.5
35	8321.0
36	9890.5
37	10692.0
38	11414.0
39	13273.5
40	15159.0
41	16466.0
42	17025.0
43	17051.0
44	17194.0
45	17248.0
46	17185.0
47	17005.0
48	15896.0
49	14184.0
50	13401.0
51	12632.0
52	11011.5
53	9422.0
54	8684.0
55	8101.0
56	6941.5
57	5891.5
58	5418.0
59	4816.0
60	3858.0
61	3170.0
62	2838.0
63	2513.5
64	1969.0
65	1673.5
66	1478.5
67	1359.0
68	1258.5
69	1131.5
70	1063.5
71	1022.0
72	961.5
73	833.0
74	740.0
75	715.0
76	645.0
77	521.5
78	422.5
79	377.0
80	347.5
81	273.5
82	206.0
83	183.0
84	145.5
85	86.5
86	56.0
87	47.0
88	36.0
89	21.5
90	18.0
91	12.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.5
99	4.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	287939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24103222753527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89767743126052	40.43599593496459
2	16.230907920842792	18.25686030916554
3	5.572494838797483	9.402085854497495
4	2.030865196532373	4.568718198718278
5	0.9550723460914533	2.685712729807857
6	0.5808992643653649	1.9602224548874412
7	0.41357093437805126	1.6281759374109497
8	0.33007267703899884	1.485090245342334
9	0.24491535617754875	1.2396863195819818
>10	1.7070761332511424	16.922695943594263
>50	0.03274133503400553	1.1718988779358448
>100	0.0037065662302647774	0.2428571940934348
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.4729578139814336E-4	0.0
4	0.0	0.0	0.0	6.945915627962867E-4	0.0
5	0.0	0.0	0.0	6.945915627962867E-4	0.0
6	0.0	0.0	0.0	6.945915627962867E-4	0.0
7	0.0	0.0	0.0	6.945915627962867E-4	0.0
8	0.0	0.0	0.0	6.945915627962867E-4	0.0
9	0.0	0.0	0.0	0.0020837746883888603	0.0
10	0.0	0.0	0.0	0.0020837746883888603	0.0
11	0.0	0.0	0.0	0.0020837746883888603	0.0
12	0.0	0.0	0.0	0.0024310704697870036	0.0
13	0.0	0.0	0.0	0.00312566203258329	0.0
14	0.0	0.0	0.0	0.00312566203258329	0.0
15	0.0	0.0	0.0	0.00312566203258329	0.0
16	0.0	3.4729578139814336E-4	0.0	0.00312566203258329	0.0
17	0.0	3.4729578139814336E-4	0.0	0.00312566203258329	0.0
18	0.0	3.4729578139814336E-4	0.0	0.00312566203258329	0.0
19	0.0	3.4729578139814336E-4	0.0	0.0034729578139814335	0.0
20	0.0	3.4729578139814336E-4	0.0	0.0041675493767777206	0.0
21	0.0	3.4729578139814336E-4	0.0	0.004862140939574007	0.0
22	0.0	3.4729578139814336E-4	0.0	0.005556732502370294	0.0
23	0.0	3.4729578139814336E-4	0.0	0.005904028283768437	0.0
24	0.0	3.4729578139814336E-4	0.0	0.00729321140936101	0.0
25	0.0	6.945915627962867E-4	0.0	0.008682394534953584	0.0
26	0.0	6.945915627962867E-4	0.0	0.008682394534953584	0.0
27	0.0	6.945915627962867E-4	0.0	0.01354453547452759	0.0
28	0.0	6.945915627962867E-4	0.0	0.024310704697870035	0.0
29	0.0	6.945915627962867E-4	0.0	0.03542416970261062	0.0
30	0.0	6.945915627962867E-4	0.0	0.04757952205154564	0.0
31	0.0	6.945915627962867E-4	0.0	0.06286053643306395	0.0
32	0.0	6.945915627962867E-4	0.0	0.07987802972157297	0.0
33	0.0	6.945915627962867E-4	0.0	0.10488332598223929	0.0
34	0.0	6.945915627962867E-4	0.0	0.14273856615463693	0.0
35	0.0	6.945915627962867E-4	0.0	0.20247344055511757	0.0
36	0.0	6.945915627962867E-4	0.0	0.2861717238720701	0.0
37	0.0	6.945915627962867E-4	0.0	0.499064037869132	0.0
38	0.0	6.945915627962867E-4	0.0	0.7473805215688045	0.0
39	0.0	0.0010418873441944301	0.0	1.0526535134177726	0.0
40	0.0	0.0010418873441944301	0.0	1.4204397459184064	0.0
41	0.0	0.0010418873441944301	0.0	1.7746814429445126	0.0
42	0.0	0.0010418873441944301	0.0	2.175808070459368	0.0
43	0.0	0.0010418873441944301	0.0	2.7026557708403516	0.0
44	0.0	0.0010418873441944301	0.0	3.289932937184612	0.0
45	0.0	0.0010418873441944301	0.0	3.9331247243339735	0.0
46	0.0	0.0010418873441944301	0.0	4.638829752135001	0.0
47	0.0	0.0010418873441944301	0.0	5.380653541201435	0.0
48	0.0	0.0010418873441944301	0.0	6.1262975838632485	0.0
49	0.0	0.0010418873441944301	0.0	6.803871653371027	0.0
50	0.0	0.0010418873441944301	0.0	7.478667356627619	0.0
51	0.0	0.0010418873441944301	0.0	8.13297260878172	0.0
52	0.0	0.0010418873441944301	0.0	8.75810501529838	0.0
53	0.0	0.0010418873441944301	0.0	9.37906987243826	0.0
54	0.0	0.0010418873441944301	0.0	10.057338533508833	0.0
55	0.0	0.0010418873441944301	0.0	10.755055758337704	0.0
56	0.0	0.0010418873441944301	0.0	11.479862054115628	0.0
57	0.0	0.0010418873441944301	0.0	12.211614265521517	0.0
58	0.0	0.0010418873441944301	0.0	12.930863828797072	0.0
59	0.0	0.0010418873441944301	0.0	13.583085306262785	0.0
60	0.0	0.0010418873441944301	0.0	14.280455235310257	0.0
61	0.0	0.0010418873441944301	0.0	14.927814571836397	0.0
62	0.0	0.0010418873441944301	0.0	15.517522808650444	0.0
63	0.0	0.0010418873441944301	0.0	16.173911835492934	0.0
64	0.0	0.0010418873441944301	0.0	16.791403734818832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGTT	15	0.0022232062	70.00001	31
CGGGTAG	30	5.8720226E-4	46.666668	10
GCGGGTA	30	5.8720226E-4	46.666668	9
ACGGTAC	35	0.001254202	40.0	3
GAAATCG	65	2.09858E-5	32.307693	48
TTACAGC	45	0.0043031503	31.111113	4
GGCTTAT	95	2.812194E-7	29.473686	1
AAATCGC	60	4.9751275E-4	29.166668	49
GGTCGTT	50	0.007201537	27.999998	11
CCTGGAC	120	7.369454E-8	26.250002	3
GCTTATG	110	1.0124768E-6	25.454544	2
TGCGGAG	70	0.0012243224	25.0	7
ATGCGGA	70	0.0012243224	25.0	6
GACTATC	85	1.3056933E-4	24.705883	38
GGGGACA	100	1.3940331E-5	24.499998	4
TAGACGG	75	0.0018297116	23.333334	27
TTAGACG	75	0.0018297116	23.333334	26
CTGACAC	90	1.9239906E-4	23.333334	3
CTTATGC	90	1.9239906E-4	23.333334	3
CGGGCTC	75	0.0018297116	23.333334	31
>>END_MODULE