FastQCFastQC Report
Wed 25 May 2016
SRR1781178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260910
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT38171.4629565750641984No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3770.14449427005480817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAG150.002222971870.057
TCTCGTA4750.056.7368438
TCGTATG5350.056.2616840
CTCGTAT5300.056.13207239
TCGTTCG252.3876011E-455.99999623
CGTATGC5450.055.87156341
TATGCCG5400.055.74074643
CCGTCTT5350.055.60747547
ATCTCGT4800.055.41666437
ATGCCGT5450.054.58716244
CGTCTTC5400.054.44444748
TGCCGTC5350.054.29906545
GCCGTCT5550.053.60360346
GTATGCC5750.052.9565242
GACCGTT200.006946044552.50000416
CGACCGT200.006946044552.50000415
ACGGGTG200.006946044552.5000047
ACCGTTG200.006946044552.50000417
CGGTAAG200.006946044552.50000428
AAAGATC200.006946044552.50000469
Produced by FastQC (version 0.11.5)