##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781178_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260910
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3001303131348	34.0	34.0	34.0	33.0	34.0
2	33.29773485109808	34.0	34.0	34.0	33.0	34.0
3	33.37712621210379	34.0	34.0	34.0	33.0	34.0
4	33.464715802383964	34.0	34.0	34.0	34.0	34.0
5	33.44050055574719	34.0	34.0	34.0	34.0	34.0
6	36.86270744701238	38.0	37.0	38.0	34.0	38.0
7	36.75861408148403	38.0	37.0	38.0	34.0	38.0
8	36.570832854240926	38.0	37.0	38.0	34.0	38.0
9	36.52758039170595	38.0	37.0	38.0	34.0	38.0
10-11	36.47057797708022	38.0	37.0	38.0	34.0	38.0
12-13	36.41410448047219	38.0	37.0	38.0	34.0	38.0
14-15	36.42058180981948	38.0	37.0	38.0	34.0	38.0
16-17	36.35921773791729	38.0	37.0	38.0	34.0	38.0
18-19	36.36701544593922	38.0	37.0	38.0	34.0	38.0
20-21	36.31185082978805	38.0	37.0	38.0	34.0	38.0
22-23	36.36271319612127	38.0	37.0	38.0	34.0	38.0
24-25	36.353098769690696	38.0	37.0	38.0	34.0	38.0
26-27	36.14530681077766	38.0	37.0	38.0	33.5	38.0
28-29	36.14739373730406	38.0	37.0	38.0	34.0	38.0
30-31	36.18291173201487	38.0	37.0	38.0	34.0	38.0
32-33	36.134184201448775	38.0	37.0	38.0	34.0	38.0
34-35	36.13945230155993	38.0	37.0	38.0	33.5	38.0
36-37	36.068517879728645	38.0	37.0	38.0	32.5	38.0
38-39	35.9879441186616	38.0	37.0	38.0	32.0	38.0
40-41	36.053221417346975	38.0	37.0	38.0	32.5	38.0
42-43	36.06095780154076	38.0	37.0	38.0	32.5	38.0
44-45	36.04255107125063	38.0	37.0	38.0	32.0	38.0
46-47	36.048754359740904	38.0	37.0	38.0	32.5	38.0
48-49	36.03046452799816	38.0	37.0	38.0	32.0	38.0
50-51	35.93457897359242	38.0	37.0	38.0	32.0	38.0
52-53	35.969573799394425	38.0	37.0	38.0	32.0	38.0
54-55	35.97444329462267	38.0	37.0	38.0	32.0	38.0
56-57	35.926183358246135	38.0	37.0	38.0	32.0	38.0
58-59	35.92506228201296	38.0	37.0	38.0	32.0	38.0
60-61	35.92101490935572	38.0	37.0	38.0	32.0	38.0
62-63	35.88667739833659	38.0	37.0	38.0	32.0	38.0
64-65	35.85952052431873	38.0	37.0	38.0	32.0	38.0
66-67	35.82336246215171	38.0	36.5	38.0	31.0	38.0
68-69	35.743447932237174	38.0	36.0	38.0	31.0	38.0
70-71	35.60927139626691	38.0	36.0	38.0	31.0	38.0
72-73	35.507420183204935	38.0	36.0	38.0	31.0	38.0
74-75	35.52088267985129	38.0	36.0	38.0	31.0	38.0
76	34.655041968494885	37.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	10.0
22	25.0
23	70.0
24	151.0
25	336.0
26	582.0
27	1118.0
28	1962.0
29	3034.0
30	4936.0
31	7255.0
32	10673.0
33	15006.0
34	21278.0
35	29497.0
36	45726.0
37	119247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50128396765168	20.196236250047907	11.046337817638266	27.256141964662145
2	20.58065999770036	21.740830171323445	33.85918515963359	23.81932467134261
3	19.35494998275267	23.419186692729294	30.389789582614696	26.836073741903338
4	13.957303284657543	18.597600705224025	37.023111417730256	30.421984592388178
5	13.367827986662068	34.63416503775248	37.15840711356406	14.839599862021386
6	29.094323713157795	38.9663102219156	18.214710053275077	13.724656011651529
7	27.423249396343568	29.652370549231538	22.956191790272506	19.96818826415239
8	23.97416733739604	36.575064198382584	20.482541872676403	18.968226591544976
9	23.58897704189184	16.87976696945307	22.167797324747998	37.363458663907096
10-11	24.258748227358094	26.246215169981983	28.04223678663141	21.452799816028513
12-13	23.686520256026984	23.775823080755817	27.715495764823117	24.82216089839408
14-15	21.93515005174198	24.952090759265648	24.435245870223447	28.677513318768927
16-17	23.157218964393852	28.64014411099613	25.42045149668468	22.782185427925338
18-19	22.538806484994826	27.466559349967422	26.90985397263424	23.08478019240351
20-21	22.12410409719827	27.311525046951058	26.39205089877736	24.17231995707332
22-23	22.049365681652677	27.784868345406462	26.9736690812924	23.19209689164846
24-25	22.79540837836802	27.91288183664865	26.712659537771643	22.579050247211683
26-27	22.257675060365642	28.29404775593116	26.997048790770762	22.45122839293243
28-29	21.829749722126405	27.074086849871605	28.526311755011307	22.569851672990687
30-31	22.77777777777778	27.04476639454218	28.10432716262313	22.073128665056917
32-33	22.357517918056036	27.34180368709517	27.274347476141198	23.0263309187076
34-35	21.987850216549766	26.562607795791653	28.293664482005287	23.15587750565329
36-37	21.96849488329309	27.7896592694799	27.93530336131233	22.306542485914683
38-39	21.546510290904912	27.408110076271512	28.150894944616915	22.894484688206663
40-41	21.6729906864436	26.357364608485685	28.573837721819785	23.39580698325093
42-43	22.475949561151353	26.314629565750643	28.477444329462266	22.73197654363574
44-45	21.19945575102526	27.426698861676442	28.52535357019662	22.84849181710168
46-47	22.30079337702656	26.1653443716224	27.93396190257177	23.599900348779272
48-49	21.674332145184163	25.92464834617301	28.66697328580737	23.734046222835463
50-51	20.865815798551225	26.01720899927178	29.262389329653903	23.854585872523092
52-53	20.4543712391246	25.873098003142847	30.547698440075123	23.12483231765743
54-55	19.841324594687823	26.24928136138898	29.673642252117588	24.235751791805605
56-57	20.536966770150627	26.13238281399716	28.82009121919436	24.51055919665785
58-59	19.609635506496492	25.98328925683186	30.377908090912577	24.02916714575907
60-61	20.582001456440917	27.02579433521138	28.331608600666897	24.06059560768081
62-63	19.044498102794066	27.826070292438004	28.429535088727913	24.699896516040013
64-65	19.39576865585834	27.891993407688474	27.76283009466866	24.949407841784524
66-67	19.371430761565293	28.01061668774673	27.422674485454756	25.19527806523322
68-69	19.525890153692842	28.164884442911347	26.934191866927293	25.375033536468518
70-71	20.021846613774866	27.635966425204096	26.70786861369821	25.634318347322832
72-73	19.919129201640413	26.56203288490284	27.025027787359623	26.493810126097124
74-75	20.121497834502318	26.419646621440346	27.042466750986932	26.416388793070407
76	20.46031198497566	26.307922272047833	26.921543827373423	26.310221915603083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	3.0
11	6.0
12	7.0
13	15.0
14	22.0
15	46.5
16	90.5
17	109.0
18	164.5
19	272.0
20	419.0
21	514.0
22	621.5
23	877.0
24	1261.0
25	1497.0
26	1724.0
27	2209.5
28	2759.5
29	3051.0
30	3362.0
31	4103.0
32	5181.0
33	5829.0
34	6222.0
35	7174.0
36	8529.0
37	9325.0
38	9997.0
39	11591.0
40	13445.5
41	15087.5
42	15797.0
43	16277.0
44	16492.0
45	16007.0
46	15787.0
47	15427.5
48	14087.0
49	12569.0
50	12032.0
51	11505.0
52	10094.0
53	8616.5
54	8023.0
55	7528.0
56	6420.5
57	5324.0
58	4840.0
59	4305.0
60	3374.5
61	2687.5
62	2396.0
63	2159.0
64	1795.0
65	1553.0
66	1335.5
67	1233.0
68	1161.5
69	1078.0
70	979.5
71	893.0
72	888.5
73	838.0
74	698.5
75	605.0
76	558.5
77	497.0
78	427.0
79	372.0
80	319.0
81	241.0
82	178.5
83	141.0
84	122.5
85	75.0
86	42.0
87	38.0
88	27.5
89	14.5
90	9.5
91	6.5
92	6.0
93	3.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	260910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.69278748259322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.71788847649023	33.314229543434806
2	19.95504452327784	20.231536624484185
3	6.71350710499884	10.209791668097573
4	2.4690590704458186	5.006539469603162
5	1.1674646660267916	2.9591019104166403
6	0.7155684922971512	2.1764496905555477
7	0.5006011510178965	1.7763807433464243
8	0.3815855409296766	1.5474907786222776
9	0.32779811251158686	1.4955300049270548
>10	1.9947076798444885	17.698381613633572
>50	0.05071916272931326	1.6336051574140773
>100	0.005299017001570042	0.4862057163363016
>500	0.0	0.0
>1k	7.570024287957204E-4	1.4647570791283846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	3817	1.4629565750641984	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG	377	0.14449427005480817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.665478517496454E-4	0.0
2	0.0	0.0	0.0	7.665478517496454E-4	0.0
3	0.0	0.0	0.0	0.0011498217776244682	0.0
4	0.0	0.0	0.0	0.0015330957034992909	0.0
5	0.0	0.0	0.0	0.0015330957034992909	0.0
6	0.0	0.0	0.0	0.0015330957034992909	0.0
7	0.0	0.0	0.0	0.0015330957034992909	0.0
8	0.0	0.0	0.0	0.0015330957034992909	0.0
9	0.0	0.0	0.0	0.0030661914069985818	0.0
10	0.0	0.0	0.0	0.0030661914069985818	0.0
11	0.0	0.0	0.0	0.0030661914069985818	0.0
12	0.0	0.0	0.0	0.0034494653328734047	0.0
13	0.0	0.0	0.0	0.00421601318462305	0.0
14	0.0	0.0	0.0	0.00421601318462305	0.0
15	0.0	0.0	0.0	0.00421601318462305	0.0
16	0.0	0.0	0.0	0.00421601318462305	0.0
17	0.0	0.0	0.0	0.004599287110497873	0.0
18	0.0	0.0	0.0	0.004599287110497873	0.0
19	0.0	0.0	0.0	0.004599287110497873	0.0
20	0.0	0.0	0.0	0.0053658349622475185	0.0
21	0.0	0.0	0.0	0.0061323828139971635	0.0
22	0.0	0.0	0.0	0.0065156567398719865	0.0
23	0.0	0.0	0.0	0.007282204591621632	0.0
24	0.0	0.0	0.0	0.009581848146870568	0.0
25	0.0	0.0	0.0	0.010731669924495037	0.0
26	0.0	0.0	0.0	0.010731669924495037	0.0
27	0.0	0.0	0.0	0.0168640527384922	0.0
28	0.0	0.0	0.0	0.028362270514736884	0.0
29	0.0	0.0	0.0	0.03909394043923192	0.0
30	0.0	0.0	0.0	0.05135870606722625	0.0
31	0.0	0.0	0.0	0.06783948487984362	0.0
32	0.0	0.0	0.0	0.08700318117358476	0.0
33	0.0	0.0	0.0	0.11344908205894753	0.0
34	0.0	0.0	0.0	0.1552259399793032	0.0
35	0.0	0.0	0.0	0.21808286382277414	0.0
36	0.0	0.0	0.0	0.3089187842551071	0.0
37	0.0	0.0	0.0	0.5339005787436281	0.0
38	0.0	0.0	0.0	0.7903108351538845	0.0
39	0.0	0.0	0.0	1.1057452761488635	0.0
40	0.0	0.0	0.0	1.4871028323943123	0.0
41	0.0	0.0	0.0	1.8561956230117664	0.0
42	0.0	0.0	0.0	2.283162776436319	0.0
43	0.0	0.0	0.0	2.8289448468820666	0.0
44	0.0	0.0	0.0	3.4372005672454105	0.0
45	0.0	0.0	0.0	4.104480472193477	0.0
46	0.0	0.0	0.0	4.837683492392013	0.0
47	0.0	0.0	0.0	5.602314974512284	0.0
48	0.0	0.0	0.0	6.366179908780806	0.0
49	0.0	0.0	0.0	7.077536315204477	0.0
50	0.0	0.0	0.0	7.770878847112031	0.0
51	0.0	0.0	0.0	8.438542025985972	0.0
52	0.0	0.0	0.0	9.08934115212142	0.0
53	0.0	0.0	0.0	9.732091525813498	0.0
54	0.0	0.0	0.0	10.421601318462304	0.0
55	0.0	0.0	0.0	11.141389751255222	0.0
56	0.0	0.0	0.0	11.88187497604538	0.0
57	0.0	0.0	0.0	12.653405388831398	0.0
58	0.0	0.0	0.0	13.382009121919436	0.0
59	0.0	0.0	0.0	14.08646659767736	0.0
60	0.0	0.0	0.0	14.794756812694033	0.0
61	0.0	0.0	0.0	15.465486182974972	0.0
62	0.0	0.0	0.0	16.077574642597064	0.0
63	0.0	0.0	0.0	16.756352765321374	0.0
64	0.0	0.0	0.0	17.407151891456824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAAG	15	0.0022229718	70.0	57
TCTCGTA	475	0.0	56.73684	38
TCGTATG	535	0.0	56.26168	40
CTCGTAT	530	0.0	56.132072	39
TCGTTCG	25	2.3876011E-4	55.999996	23
CGTATGC	545	0.0	55.871563	41
TATGCCG	540	0.0	55.740746	43
CCGTCTT	535	0.0	55.607475	47
ATCTCGT	480	0.0	55.416664	37
ATGCCGT	545	0.0	54.587162	44
CGTCTTC	540	0.0	54.444447	48
TGCCGTC	535	0.0	54.299065	45
GCCGTCT	555	0.0	53.603603	46
GTATGCC	575	0.0	52.95652	42
GACCGTT	20	0.0069460445	52.500004	16
CGACCGT	20	0.0069460445	52.500004	15
ACGGGTG	20	0.0069460445	52.500004	7
ACCGTTG	20	0.0069460445	52.500004	17
CGGTAAG	20	0.0069460445	52.500004	28
AAAGATC	20	0.0069460445	52.500004	69
>>END_MODULE