FastQCFastQC Report
Wed 25 May 2016
SRR1781177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193921
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAC150.002222108470.022
AACCGGC200.006943367452.523
GGTACTC200.006943367452.545
CTGTAGA200.006943367452.51
TATATCG200.006943367452.522
TAACCGG200.006943367452.522
CGGCTTT200.006943367452.526
TAGGCGG200.006943367452.534
TAAGATC200.006943367452.532
GACACGG305.867253E-446.6666647
ACACTTG305.867253E-446.66666449
ACCTTTG350.001253189340.042
TTGGCGG400.002414493135.039
GCGACTG400.002414493135.038
CAGTTGG400.002414493135.01
TAATCGA400.002414493135.032
GAACTAT552.9843504E-431.8181846
ACCGGCT450.004299717531.11111324
TAGACTG901.745957E-731.1111135
CCCTAAG450.004299717531.1111131
Produced by FastQC (version 0.11.5)