##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781177_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193921
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4248946736042	34.0	33.0	34.0	23.0	34.0
2	31.597470103805158	34.0	33.0	34.0	24.0	34.0
3	31.52988588136406	34.0	34.0	34.0	23.0	34.0
4	31.72094306444377	34.0	34.0	34.0	23.0	34.0
5	31.717090980347667	34.0	34.0	34.0	23.0	34.0
6	34.585372393912984	38.0	35.0	38.0	26.0	38.0
7	34.50880513198674	38.0	34.0	38.0	25.0	38.0
8	34.386172719818894	38.0	34.0	38.0	25.0	38.0
9	34.607350415891005	38.0	35.0	38.0	26.0	38.0
10-11	34.47409512120915	38.0	34.5	38.0	25.5	38.0
12-13	34.43337493102862	38.0	34.0	38.0	25.5	38.0
14-15	34.30767683747506	38.0	34.0	38.0	24.0	38.0
16-17	34.25456500327453	37.5	34.0	38.0	24.5	38.0
18-19	34.319031976939065	37.5	34.0	38.0	24.5	38.0
20-21	34.22708989743246	37.5	34.0	38.0	24.5	38.0
22-23	34.288207569061626	37.5	34.0	38.0	24.5	38.0
24-25	34.27950815022612	37.5	34.0	38.0	24.0	38.0
26-27	34.18990723026387	37.0	34.0	38.0	24.0	38.0
28-29	34.27486192831101	37.5	34.0	38.0	24.0	38.0
30-31	34.336838196997746	38.0	34.0	38.0	24.5	38.0
32-33	34.2893961974206	37.5	34.0	38.0	24.5	38.0
34-35	34.32881431098231	38.0	34.0	38.0	24.5	38.0
36-37	34.1976861711728	37.0	34.0	38.0	24.0	38.0
38-39	34.20182445428809	37.0	34.0	38.0	24.0	38.0
40-41	34.213177015382556	37.0	34.0	38.0	24.0	38.0
42-43	34.252035622753596	37.0	34.0	38.0	24.0	38.0
44-45	34.26606968817198	37.0	34.0	38.0	24.0	38.0
46-47	34.24839496495996	37.0	34.0	38.0	24.0	38.0
48-49	34.24094863372198	37.0	34.0	38.0	24.0	38.0
50-51	34.09775888119388	37.0	34.0	38.0	23.5	38.0
52-53	34.18363147879806	37.0	34.0	38.0	24.0	38.0
54-55	34.148011303572076	37.0	34.0	38.0	24.0	38.0
56-57	34.12184600945746	37.0	34.0	38.0	24.0	38.0
58-59	34.12407629911149	37.0	34.0	38.0	24.0	38.0
60-61	34.0936515385131	37.0	34.0	38.0	24.0	38.0
62-63	34.05717276622954	37.0	34.0	38.0	23.5	38.0
64-65	34.082224204701916	37.0	34.0	38.0	24.0	38.0
66-67	33.97665286379505	37.0	34.0	38.0	23.0	38.0
68-69	33.768555236410705	37.0	34.0	38.0	22.5	38.0
70-71	33.91634480020215	37.0	34.0	38.0	23.0	38.0
72-73	33.87943286183549	37.0	34.0	38.0	23.0	38.0
74-75	33.89679818070245	37.0	34.0	38.0	23.0	38.0
76	32.52934958049928	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	28.0
14	107.0
15	304.0
16	688.0
17	1178.0
18	1473.0
19	1623.0
20	1490.0
21	1499.0
22	1551.0
23	1749.0
24	1949.0
25	2341.0
26	2807.0
27	3345.0
28	4045.0
29	4887.0
30	5904.0
31	7230.0
32	8986.0
33	10911.0
34	14165.0
35	19485.0
36	29856.0
37	66317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.77151520464519	17.405541431820172	9.037700919446578	25.78524244408806
2	23.98554050360714	19.51361636955255	31.16733102655205	25.333512100288264
3	20.157177407294725	21.290112984153343	28.57142857142857	29.981281037123363
4	14.314592024587332	15.417102840847562	36.23021745968719	34.038087674877914
5	14.466200153670824	33.49972411445898	35.17257027346187	16.86150545840832
6	31.41897989387431	36.271471372362974	17.353458367067002	14.956090366695715
7	30.344831142578677	28.09443020611486	21.760923262565683	19.799815388740775
8	26.694891218589014	33.16608309569361	19.015991047900947	21.123034637816428
9	24.959648516664	14.921024540921305	20.462456361095498	39.6568705813192
10-11	26.136416375740634	23.45465421486069	27.081130976016006	23.327798433382664
12-13	26.0923262565684	21.973380912845954	25.953609975196084	25.980682855389563
14-15	24.327690141861893	23.24683762975645	23.279582922942847	29.14588930543881
16-17	24.88126608258002	25.92060684505546	24.277927609696732	24.92019946266779
18-19	23.953826558237633	25.513482294336352	25.83062174803141	24.7020693993946
20-21	24.180207404045976	25.64549481489885	25.418082621273612	24.75621515978156
22-23	24.43340329309358	25.136008993352966	25.80767425910551	24.622913454447946
24-25	23.864872809030484	25.445929012329767	25.726713455479295	24.962484723160465
26-27	23.401539802290625	25.60501441308574	26.047204789579258	24.946240995044374
28-29	24.08790177443392	25.052211983230283	26.779977413482808	24.079908828852986
30-31	24.32201772886897	25.376570871643604	25.71304809690544	24.58836330258198
32-33	23.655509202200896	25.917770638559002	25.636728358455247	24.789991800784854
34-35	24.511012216314892	24.762145409728703	26.16503627765946	24.561806096296944
36-37	24.470789651456005	24.664940877986396	25.65606613002202	25.20820334053558
38-39	23.748846179629847	25.413957230006034	26.19855508170853	24.63864150865559
40-41	24.018543633747765	24.50224575987129	26.867384140964617	24.61182646541633
42-43	23.629983343732757	24.783545876929264	26.555143589399805	25.031327189938168
44-45	23.129521815584695	24.723985540503605	26.857844173658346	25.28864847025335
46-47	23.269527281728124	24.642766899923167	26.887753260348283	25.199952558000422
48-49	22.875036741765978	24.528287292247875	27.37970616900697	25.216969796979182
50-51	22.83249364431908	24.596871922071358	27.226035344289684	25.344599089319882
52-53	22.720850243140248	24.404267717266308	27.714120698635007	25.16076134095843
54-55	22.03655096663074	24.764465942316715	27.843554849655273	25.35542824139727
56-57	21.877320544554454	25.020884900990097	27.398927392739274	25.70286716171617
58-59	22.198071369636963	24.570699257425744	27.37108085808581	25.860148514851485
60-61	21.835808580858085	24.468079620462046	27.516501650165015	26.179610148514854
62-63	21.32967202970297	24.340965346534656	27.984220297029704	26.345142326732674
64-65	22.170998349834985	24.54775165016502	27.046462458745875	26.23478754125412
66-67	21.992832095709574	24.52145214521452	26.631600660066006	26.854115099009903
68-69	21.99618399339934	24.68878919141914	26.484375	26.83065181518152
70-71	22.331831858931885	24.464664643500818	26.716876245677994	26.486627251889306
72-73	22.251586986453244	24.491674444748117	26.370016656267243	26.886721912531392
74-75	22.20265521180071	24.900410219651867	26.257409608576708	26.63952495997071
76	22.627771102665516	24.833824083002874	25.836809834932783	26.70159497939883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	0.5
10	0.5
11	1.5
12	2.0
13	1.5
14	5.5
15	11.5
16	24.5
17	36.0
18	37.5
19	51.0
20	84.5
21	106.0
22	141.0
23	227.0
24	359.0
25	440.0
26	562.5
27	732.5
28	992.5
29	1205.0
30	1354.0
31	1717.5
32	2423.0
33	2914.0
34	3282.0
35	4019.5
36	5068.5
37	5748.0
38	6171.5
39	7444.5
40	9095.5
41	10088.0
42	10279.0
43	10663.0
44	11282.5
45	11627.0
46	11736.0
47	11401.0
48	10620.0
49	9818.0
50	9462.0
51	8661.0
52	7133.0
53	6128.0
54	5850.0
55	5519.0
56	4852.0
57	4229.0
58	3942.0
59	3661.0
60	3237.5
61	2949.0
62	2803.0
63	2707.5
64	2526.5
65	2445.0
66	2383.0
67	2317.0
68	2204.0
69	2134.5
70	2095.5
71	2013.0
72	1939.0
73	1830.5
74	1713.5
75	1631.0
76	1486.0
77	1221.5
78	988.5
79	875.0
80	777.0
81	601.5
82	450.0
83	376.0
84	310.5
85	194.0
86	107.0
87	71.0
88	55.0
89	33.0
90	17.0
91	6.0
92	5.0
93	3.5
94	1.5
95	2.5
96	4.0
97	2.0
98	1.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.156739084472543E-4
58-59	5.156739084472543E-4
60-61	5.156739084472543E-4
62-63	5.156739084472543E-4
64-65	5.156739084472543E-4
66-67	5.156739084472543E-4
68-69	5.156739084472543E-4
70-71	2.5783695422362715E-4
72-73	0.0
74-75	2.5783695422362715E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	193921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.093914278268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12099919874456	37.57065149922434
2	14.354999840517715	14.956162623129613
3	5.44499993950672	8.509540802815124
4	2.24800107043702	4.6842870024320336
5	1.1900000020924777	3.0995879050072133
6	0.7559999918577938	2.362979926212673
7	0.6119999932062444	2.2317032629070686
8	0.4739999947340304	1.9754012074859253
9	0.3909999956560405	1.8331848230858039
>10	2.3769999735918224	21.766920900203683
>50	0.03099999965559381	1.0095800474965233
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001547021725341763	0.0
2	0.0	0.0	0.0	0.001547021725341763	0.0
3	0.0	0.0	0.0	0.001547021725341763	0.0
4	0.0	0.0	0.0	0.002062695633789017	0.0
5	0.0	0.0	0.0	0.0025783695422362716	0.0
6	0.0	0.0	0.0	0.0025783695422362716	0.0
7	0.0	0.0	0.0	0.0025783695422362716	0.0
8	0.0	0.0	0.0	0.0025783695422362716	0.0
9	0.0	0.0	0.0	0.003094043450683526	0.0
10	0.0	0.0	0.0	0.00360971735913078	0.0
11	0.0	0.0	0.0	0.004125391267578034	0.0
12	0.0	0.0	0.0	0.004125391267578034	0.0
13	0.0	0.0	0.0	0.004125391267578034	0.0
14	0.0	0.0	0.0	0.004125391267578034	0.0
15	0.0	0.0	0.0	0.004125391267578034	0.0
16	0.0	0.0	0.0	0.004641065176025289	0.0
17	0.0	0.0	0.0	0.005156739084472543	0.0
18	0.0	0.0	0.0	0.005156739084472543	0.0
19	0.0	0.0	0.0	0.0056724129929197975	0.0
20	0.0	0.0	0.0	0.0056724129929197975	0.0
21	0.0	0.0	0.0	0.0056724129929197975	0.0
22	0.0	0.0	0.0	0.01082915207739234	0.0
23	0.0	0.0	0.0	0.011344825985839595	0.0
24	0.0	0.0	0.0	0.013407521619628611	0.0
25	0.0	0.0	0.0	0.013407521619628611	0.0
26	0.0	0.0	0.0	0.013407521619628611	0.0
27	0.0	0.0	0.0	0.02526802151391546	0.0
28	0.0	0.0	0.0	0.037644195316649566	0.0
29	0.0	0.0	0.0	0.05208306475317268	0.0
30	0.0	0.0	0.0	0.06136519510522326	0.0
31	0.0	0.0	0.0	0.07941378190087717	0.0
32	0.0	0.0	0.0	0.10880719468237066	0.0
33	0.0	0.0	0.0	0.150061107358151	0.0
34	0.0	0.0	0.0	0.21142630246337427	0.0
35	0.0	0.0	0.0	0.29754384517406574	0.0
36	0.0	0.0	0.0	0.44812062644066397	0.0
37	0.0	0.0	0.0	0.7013165154882658	0.0
38	0.0	0.0	0.0	1.0076268171059348	0.0
39	0.0	0.0	0.0	1.3051706622800006	0.0
40	0.0	0.0	0.0	1.6563445939325807	0.0
41	0.0	0.0	0.0	2.03588059054976	0.0
42	0.0	0.0	0.0	2.4277927609696732	0.0
43	0.0	0.0	0.0	2.9084008436425144	0.0
44	0.0	0.0	0.0	3.4828615776527556	0.0
45	0.0	0.0	0.0	4.154269006451081	0.0
46	0.0	0.0	0.0	4.872087087009659	0.0
47	0.0	0.0	0.0	5.6497233409481185	0.0
48	0.0	0.0	0.0	6.433032007879497	0.0
49	0.0	0.0	0.0	7.141567958086025	0.0
50	0.0	0.0	0.0	7.735108626708814	0.0
51	0.0	0.0	0.0	8.340509795225891	0.0
52	0.0	0.0	0.0	8.95106770282744	0.0
53	0.0	0.0	0.0	9.586893631942905	0.0
54	0.0	0.0	0.0	10.246440560846942	0.0
55	0.0	0.0	0.0	11.006543901898196	0.0
56	0.0	0.0	0.0	11.769225612491685	0.0
57	0.0	0.0	0.0	12.50251391030368	0.0
58	0.0	0.0	0.0	13.292010664136427	0.0
59	0.0	0.0	0.0	13.960839723392516	0.0
60	0.0	0.0	0.0	14.669891347507491	0.0
61	0.0	0.0	0.0	15.274261168207673	0.0
62	0.0	0.0	0.0	15.905961706055558	0.0
63	0.0	0.0	0.0	16.514972591931766	0.0
64	0.0	0.0	0.0	17.11366999963903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGAC	15	0.0022221084	70.0	22
AACCGGC	20	0.0069433674	52.5	23
GGTACTC	20	0.0069433674	52.5	45
CTGTAGA	20	0.0069433674	52.5	1
TATATCG	20	0.0069433674	52.5	22
TAACCGG	20	0.0069433674	52.5	22
CGGCTTT	20	0.0069433674	52.5	26
TAGGCGG	20	0.0069433674	52.5	34
TAAGATC	20	0.0069433674	52.5	32
GACACGG	30	5.867253E-4	46.666664	7
ACACTTG	30	5.867253E-4	46.666664	49
ACCTTTG	35	0.0012531893	40.0	42
TTGGCGG	40	0.0024144931	35.0	39
GCGACTG	40	0.0024144931	35.0	38
CAGTTGG	40	0.0024144931	35.0	1
TAATCGA	40	0.0024144931	35.0	32
GAACTAT	55	2.9843504E-4	31.818184	6
ACCGGCT	45	0.0042997175	31.111113	24
TAGACTG	90	1.745957E-7	31.111113	5
CCCTAAG	45	0.0042997175	31.111113	1
>>END_MODULE