FastQCFastQC Report
Wed 25 May 2016
SRR1781177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174380
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT35472.03406353939672No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3700.21218029590549373No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2630.15082004817066177No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAACCTCGTATGCCGT2200.12616125702488817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT252.8308896E-670.0000139
CGTATAA150.002221731570.033
TATGCCG6000.054.2543
CGTATGC6100.053.9344341
TCGTATG6100.053.9344340
CGTCTTC5900.052.7966148
CTCGTAT5850.052.64957439
TCGTATA200.00694219852.50000432
CGAAGTA200.00694219852.50000412
CCGTCTT6000.051.91666847
GCCGTCT6000.051.91666846
ATGCCGT6250.051.5244
TCTCGTA5250.051.33333238
CTGCTTG6250.050.454
TGCCGTC6450.049.92247845
ATCTCGT5450.049.44954337
AATCTCG5400.049.2592636
GTATGCC6650.048.9473742
ATCTTAA6700.048.58209231
TCTTCTG6350.048.50393750
Produced by FastQC (version 0.11.5)