##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781177_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174380
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3255763275605	34.0	34.0	34.0	33.0	34.0
2	33.318786558091524	34.0	34.0	34.0	33.0	34.0
3	33.38540543640326	34.0	34.0	34.0	34.0	34.0
4	33.4656325266659	34.0	34.0	34.0	34.0	34.0
5	33.461262759490765	34.0	34.0	34.0	34.0	34.0
6	36.878913866269066	38.0	37.0	38.0	35.0	38.0
7	36.77459571051726	38.0	37.0	38.0	34.0	38.0
8	36.58261268494093	38.0	37.0	38.0	34.0	38.0
9	36.550550521848834	38.0	37.0	38.0	34.0	38.0
10-11	36.49197155637114	38.0	37.0	38.0	34.0	38.0
12-13	36.4216165844707	38.0	37.0	38.0	34.0	38.0
14-15	36.42397924073862	38.0	37.0	38.0	34.0	38.0
16-17	36.37087682073633	38.0	37.0	38.0	34.0	38.0
18-19	36.3623896089001	38.0	37.0	38.0	34.0	38.0
20-21	36.31061474939787	38.0	37.0	38.0	34.0	38.0
22-23	36.38219979355431	38.0	37.0	38.0	34.0	38.0
24-25	36.35761841954353	38.0	37.0	38.0	34.0	38.0
26-27	36.16027354054364	38.0	37.0	38.0	33.5	38.0
28-29	36.138180984057804	38.0	37.0	38.0	33.5	38.0
30-31	36.15606720954238	38.0	37.0	38.0	34.0	38.0
32-33	36.1076356233513	38.0	37.0	38.0	33.0	38.0
34-35	36.12523511870627	38.0	37.0	38.0	33.5	38.0
36-37	36.06299460947356	38.0	37.0	38.0	32.5	38.0
38-39	35.89385537332263	38.0	37.0	38.0	32.0	38.0
40-41	36.00765856176167	38.0	37.0	38.0	32.0	38.0
42-43	36.05712811102191	38.0	37.0	38.0	32.5	38.0
44-45	36.01398669572198	38.0	37.0	38.0	32.0	38.0
46-47	36.053521045991516	38.0	37.0	38.0	32.0	38.0
48-49	36.01936575295332	38.0	37.0	38.0	32.0	38.0
50-51	35.91828764766602	38.0	37.0	38.0	32.0	38.0
52-53	35.94873552012845	38.0	37.0	38.0	32.0	38.0
54-55	35.96720667507742	38.0	37.0	38.0	32.0	38.0
56-57	35.927216423901825	38.0	37.0	38.0	32.0	38.0
58-59	35.88644626677372	38.0	37.0	38.0	32.0	38.0
60-61	35.89129487326528	38.0	37.0	38.0	31.5	38.0
62-63	35.88562908590435	38.0	37.0	38.0	31.5	38.0
64-65	35.82480789081317	38.0	36.0	38.0	31.0	38.0
66-67	35.77401651565547	38.0	36.0	38.0	31.0	38.0
68-69	35.680046450281	38.0	36.0	38.0	31.0	38.0
70-71	35.470274687464155	38.0	36.0	38.0	30.5	38.0
72-73	35.321057460717974	38.0	36.0	38.0	28.5	38.0
74-75	35.32668023855947	38.0	36.0	38.0	29.0	38.0
76	34.45451313223994	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	5.0
22	24.0
23	49.0
24	130.0
25	251.0
26	467.0
27	779.0
28	1374.0
29	2243.0
30	3379.0
31	4932.0
32	7201.0
33	10164.0
34	13997.0
35	19764.0
36	29765.0
37	79852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64055510953091	17.140153687349464	8.94598004358298	28.273311159536647
2	23.77738272737699	18.86684252781282	31.747906870053903	25.60786787475628
3	20.319990824635852	21.052299575639406	29.341667622433764	29.28604197729097
4	14.239591696295447	17.49569904805597	35.11182475054479	33.1528845051038
5	14.4460373896089	32.910310815460484	36.63493519899071	16.0087165959399
6	31.530565431815578	38.019268264709254	16.311503612799633	14.138662690675535
7	29.694919142103455	28.30657185457048	20.320564284895056	21.677944718431014
8	26.041977290973733	35.483426998509	17.7268035325152	20.747792178002065
9	24.563596742745727	14.368620254616355	19.393279045762128	41.67450395687579
10-11	25.43869709829109	24.66509920862484	27.173987842642504	22.722215850441565
12-13	25.23483197614405	21.733283633444202	26.27279504530336	26.759089345108382
14-15	23.698531941736437	22.548744122032343	22.348606491570134	31.404117444661082
16-17	25.562851244408762	26.56497304736782	23.22198646633788	24.65018924188554
18-19	24.975627938983827	25.311388920747792	24.4176511067783	25.29533203349008
20-21	23.513877738272736	25.241140038995297	24.073288221126276	27.17169400160569
22-23	23.73437320793669	26.126562679206334	24.590262644798717	25.54880146805826
24-25	25.0992086248423	26.24698933363918	24.36661314370914	24.28718889780938
26-27	24.54209198302558	26.593932790457618	24.543238903543983	24.32073632297282
28-29	23.73695377910311	24.902798486064917	25.277554765454752	26.082692969377224
30-31	23.699965592384448	27.417421722674618	24.73878885193256	24.143823833008373
32-33	23.16062621860305	25.504931758229155	26.22405092327102	25.110391099896777
34-35	24.056944603738962	24.379515999541233	27.78529647895401	23.778242917765798
36-37	23.773655235692168	27.188037618993004	24.731333868562906	24.30697327675192
38-39	23.29424245899759	25.49575639408189	26.38605344649616	24.823947700424362
40-41	23.17066177313912	24.124326184195436	27.10861337309324	25.596398669572203
42-43	24.554421378598462	24.22009404748251	26.78804908819819	24.43743548572084
44-45	22.708166074091064	25.840119279733916	27.04295217341438	24.408762472760635
46-47	24.154146117674046	23.993863975226517	26.01502465879115	25.836965248308292
48-49	23.819245326298887	23.74268838169515	26.479240738616816	25.958825553389147
50-51	22.32538135107237	24.054650762702146	27.571969262530104	26.047998623695378
52-53	21.775146232366097	23.88060557403372	29.330771877508887	25.013476316091293
54-55	21.319245326298887	24.178231448560613	28.317754329624954	26.184768895515543
56-57	22.522938410368162	24.073288221126276	26.845968574377792	26.557804794127765
58-59	21.334728753297398	24.00992086248423	28.91616011010437	25.739190274114005
60-61	22.461291432503728	25.26809267117789	26.447987154490193	25.82262874182819
62-63	20.350097488244064	26.955212753756165	26.566119967886227	26.12856979011354
64-65	20.81259318729212	27.29126046564973	25.8518752150476	26.04427113201055
66-67	21.01559811905035	27.272336277096	25.12931528845051	26.582750315403143
68-69	20.83667851817869	27.421149214359446	24.72015139350843	27.022020873953434
70-71	21.061761669916272	26.90876247276064	24.72043812363803	27.309037733685056
72-73	20.897178575524716	25.570306227778417	24.91025346943457	28.6222617272623
74-75	20.713671292579424	25.666360821195088	25.235118706273656	28.384849179951832
76	21.131437091409566	25.5826356233513	25.13763046220897	28.148296823030165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	3.0
14	4.0
15	8.0
16	17.0
17	25.0
18	26.5
19	63.5
20	95.5
21	92.0
22	115.5
23	201.5
24	311.5
25	359.0
26	460.0
27	653.0
28	858.5
29	972.0
30	1125.5
31	1439.0
32	2099.5
33	2600.0
34	2786.5
35	3363.5
36	4241.5
37	4729.0
38	5485.0
39	7232.0
40	9063.5
41	10076.5
42	10249.0
43	10348.5
44	10303.5
45	10032.0
46	9905.0
47	9812.0
48	9243.5
49	8418.0
50	8068.0
51	7489.0
52	6286.5
53	5432.5
54	5202.0
55	4988.0
56	4359.5
57	3638.5
58	3332.0
59	3120.0
60	2775.5
61	2573.5
62	2504.0
63	2399.0
64	2247.5
65	2214.5
66	2154.0
67	2080.0
68	2027.0
69	1924.0
70	1869.0
71	1864.0
72	1807.5
73	1660.0
74	1556.5
75	1544.0
76	1393.5
77	1215.0
78	1036.0
79	885.0
80	773.0
81	570.5
82	395.5
83	311.0
84	246.5
85	151.0
86	97.0
87	74.0
88	49.5
89	17.5
90	8.5
91	6.0
92	5.0
93	5.5
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	174380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.92315632526666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.38894775138185	28.476316091294873
2	19.17101752683917	17.22445234545246
3	7.128176978949921	9.60660626218603
4	2.9181612775572208	5.2437206101617155
5	1.610988421818553	3.6185342355774743
6	1.0071868976345788	2.714760867071912
7	0.7531562352400526	2.3683908705126733
8	0.5680585164098702	2.0415185227663724
9	0.46976524503108363	1.899300378483771
>10	2.932203173468476	22.948732652827157
>50	0.045955295709562534	1.266200252322514
>100	0.005106143967729171	0.5574033719463242
>500	0.0	0.0
>1k	0.0012765359919322927	2.03406353939672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	3547	2.03406353939672	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG	370	0.21218029590549373	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC	263	0.15082004817066177	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAACCTCGTATGCCGT	220	0.12616125702488817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0017203807776121115	0.0
2	0.0	0.0	0.0	0.0017203807776121115	0.0
3	0.0	0.0	0.0	0.0017203807776121115	0.0
4	0.0	0.0	0.0	0.0022938410368161487	0.0
5	0.0	0.0	0.0	0.002867301296020186	0.0
6	0.0	0.0	0.0	0.002867301296020186	0.0
7	0.0	0.0	0.0	0.002867301296020186	0.0
8	0.0	0.0	0.0	0.002867301296020186	0.0
9	0.0	0.0	0.0	0.003440761555224223	0.0
10	0.0	0.0	0.0	0.00401422181442826	0.0
11	0.0	0.0	0.0	0.004587682073632297	0.0
12	0.0	0.0	0.0	0.004587682073632297	0.0
13	0.0	0.0	0.0	0.004587682073632297	0.0
14	0.0	0.0	0.0	0.004587682073632297	0.0
15	0.0	0.0	0.0	0.004587682073632297	0.0
16	0.0	0.0	0.0	0.004587682073632297	0.0
17	0.0	0.0	0.0	0.0051611423328363345	0.0
18	0.0	0.0	0.0	0.0051611423328363345	0.0
19	0.0	0.0	0.0	0.006308062851244409	0.0
20	0.0	0.0	0.0	0.006308062851244409	0.0
21	0.0	0.0	0.0	0.006308062851244409	0.0
22	0.0	0.0	0.0	0.007454983369652483	0.0
23	0.0	0.0	0.0	0.007454983369652483	0.0
24	0.0	0.0	0.0	0.00802844362885652	0.0
25	0.0	0.0	0.0	0.008601903888060557	0.0
26	0.0	0.0	0.0	0.008601903888060557	0.0
27	0.0	0.0	0.0	0.021218029590549376	0.0
28	0.0	0.0	0.0	0.03440761555224223	0.0
29	0.0	0.0	0.0	0.053331804105975454	0.0
30	0.0	0.0	0.0	0.0648010092900562	0.0
31	0.0	0.0	0.0	0.08372519784378943	0.0
32	0.0	0.0	0.0	0.11354513132239935	0.0
33	0.0	0.0	0.0	0.1588484917995183	0.0
34	0.0	0.0	0.0	0.2236495010895745	0.0
35	0.0	0.0	0.0	0.3199908246358527	0.0
36	0.0	0.0	0.0	0.478265856176167	0.0
37	0.0	0.0	0.0	0.755247161371717	0.0
38	0.0	0.0	0.0	1.0941621745613028	0.0
39	0.0	0.0	0.0	1.4112856979011354	0.0
40	0.0	0.0	0.0	1.7811675650877394	0.0
41	0.0	0.0	0.0	2.1894712696410137	0.0
42	0.0	0.0	0.0	2.6035095767863288	0.0
43	0.0	0.0	0.0	3.111595366441106	0.0
44	0.0	0.0	0.0	3.7114347975685287	0.0
45	0.0	0.0	0.0	4.417937836907902	0.0
46	0.0	0.0	0.0	5.1634361738731505	0.0
47	0.0	0.0	0.0	5.969721298314027	0.0
48	0.0	0.0	0.0	6.773712581718087	0.0
49	0.0	0.0	0.0	7.506594792980846	0.0
50	0.0	0.0	0.0	8.133386856290858	0.0
51	0.0	0.0	0.0	8.773368505562564	0.0
52	0.0	0.0	0.0	9.393279045762128	0.0
53	0.0	0.0	0.0	10.045303360477119	0.0
54	0.0	0.0	0.0	10.739190274114003	0.0
55	0.0	0.0	0.0	11.524257368964332	0.0
56	0.0	0.0	0.0	12.317352907443514	0.0
57	0.0	0.0	0.0	13.09381809840578	0.0
58	0.0	0.0	0.0	13.920174331918798	0.0
59	0.0	0.0	0.0	14.624956990480559	0.0
60	0.0	0.0	0.0	15.345796536300034	0.0
61	0.0	0.0	0.0	15.978323202202088	0.0
62	0.0	0.0	0.0	16.622892533547425	0.0
63	0.0	0.0	0.0	17.239362312191766	0.0
64	0.0	0.0	0.0	17.87074205757541	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	25	2.8308896E-6	70.00001	39
CGTATAA	15	0.0022217315	70.0	33
TATGCCG	600	0.0	54.25	43
CGTATGC	610	0.0	53.93443	41
TCGTATG	610	0.0	53.93443	40
CGTCTTC	590	0.0	52.79661	48
CTCGTAT	585	0.0	52.649574	39
TCGTATA	20	0.006942198	52.500004	32
CGAAGTA	20	0.006942198	52.500004	12
CCGTCTT	600	0.0	51.916668	47
GCCGTCT	600	0.0	51.916668	46
ATGCCGT	625	0.0	51.52	44
TCTCGTA	525	0.0	51.333332	38
CTGCTTG	625	0.0	50.4	54
TGCCGTC	645	0.0	49.922478	45
ATCTCGT	545	0.0	49.449543	37
AATCTCG	540	0.0	49.25926	36
GTATGCC	665	0.0	48.94737	42
ATCTTAA	670	0.0	48.582092	31
TCTTCTG	635	0.0	48.503937	50
>>END_MODULE