FastQCFastQC Report
Wed 25 May 2016
SRR1781176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121444
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAAG200.00693714452.529
ACCCGCA200.00693714452.543
GTGACGT200.00693714452.564
GCATCAG350.001251338440.025
CGTGCCA400.002410948535.027
ATACTGG400.002410948535.06
TACTAAA400.002410948535.041
GCAATTT400.002410948535.013
GTGAAAC400.002410948535.026
TGTGCAT552.9778222E-431.8181810
GGGTATA450.004293445531.1111091
GAAACCC450.004293445531.11110928
GCATGGT450.004293445531.11110913
GTTGCAC450.004293445531.11110937
CGATAAA2250.028.070
GTATTAC500.007185390727.99999816
CTATATC500.007185390727.9999984
TCAGATA500.007185390727.99999853
TTGGTCA500.007185390727.99999816
CCTATAT500.007185390727.9999983