FastQCFastQC Report
Wed 25 May 2016
SRR1781176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109712
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT45284.127169316027417No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG4650.42383695493656115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC2990.27253171941082105No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTACCTCGTATGCCGT2450.2233119439988333No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTAACTCGTATGCCGT1630.14857080355840746No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT1210.11028875601575033RNA PCR Primer, Index 45 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATGA150.002219529470.055
TAACTCG150.002219529470.036
TCGTATG6450.059.68992240
CTCGTAT6300.059.44444339
TCTCGTA5600.059.37538
CGTATGC6500.059.2307741
TATGCCG6500.059.2307743
GTATGCC6700.057.4626942
ATCTCGT5800.057.32758737
CTGCTTG6350.056.77165654
TCTTCTG6050.056.69421450
GCCGTCT6300.056.66666446
ATGCCGT6700.056.4179144
CTTGAAA6400.056.32812557
TATCTCG5950.055.8823536
CATTTAT6150.055.77235832
TGAAAAA6550.055.57251759
TTATCTC6050.055.5371935
TTGAAAA6500.055.4615458
CGTCTTC6400.055.23437548