##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781176_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109712
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.33563329444363	34.0	34.0	34.0	33.0	34.0
2	33.33319964999271	34.0	34.0	34.0	33.0	34.0
3	33.405005833454865	34.0	34.0	34.0	34.0	34.0
4	33.46570110835643	34.0	34.0	34.0	34.0	34.0
5	33.47724952603179	34.0	34.0	34.0	34.0	34.0
6	36.90941738369549	38.0	37.0	38.0	35.0	38.0
7	36.794352486510135	38.0	37.0	38.0	34.0	38.0
8	36.62727869330611	38.0	37.0	38.0	34.0	38.0
9	36.588550021875456	38.0	37.0	38.0	34.0	38.0
10-11	36.494872940061256	38.0	37.0	38.0	34.0	38.0
12-13	36.43725390112294	38.0	37.0	38.0	34.0	38.0
14-15	36.463235562199216	38.0	37.0	38.0	34.0	38.0
16-17	36.39507073064023	38.0	37.0	38.0	34.0	38.0
18-19	36.37806712119003	38.0	37.0	38.0	34.0	38.0
20-21	36.291763890914396	38.0	37.0	38.0	34.0	38.0
22-23	36.375127606825146	38.0	37.0	38.0	34.0	38.0
24-25	36.361974989062276	38.0	37.0	38.0	34.0	38.0
26-27	36.11215272714015	38.0	37.0	38.0	33.0	38.0
28-29	36.14892172232755	38.0	37.0	38.0	33.0	38.0
30-31	36.173499708327256	38.0	37.0	38.0	33.5	38.0
32-33	36.1084065553449	38.0	37.0	38.0	33.0	38.0
34-35	36.10362585678868	38.0	37.0	38.0	33.5	38.0
36-37	36.043158451217735	38.0	37.0	38.0	32.0	38.0
38-39	35.71996682222546	38.0	36.0	38.0	31.0	38.0
40-41	35.954941118564975	38.0	36.5	38.0	32.0	38.0
42-43	36.04155880851684	38.0	37.0	38.0	32.5	38.0
44-45	36.03660492926936	38.0	37.0	38.0	32.0	38.0
46-47	36.01243255067814	38.0	37.0	38.0	32.0	38.0
48-49	35.9869111856497	38.0	37.0	38.0	32.0	38.0
50-51	35.85517536823684	38.0	36.0	38.0	31.0	38.0
52-53	35.887327730786055	38.0	37.0	38.0	31.5	38.0
54-55	35.891953478197465	38.0	37.0	38.0	31.5	38.0
56-57	35.84346288464343	38.0	36.5	38.0	31.5	38.0
58-59	35.772294735306986	38.0	36.0	38.0	31.0	38.0
60-61	35.7622684847601	38.0	36.0	38.0	31.0	38.0
62-63	35.757378408925184	38.0	36.0	38.0	31.0	38.0
64-65	35.74025630742307	38.0	36.0	38.0	31.0	38.0
66-67	35.65288664868018	38.0	36.0	38.0	31.0	38.0
68-69	35.455556365757616	38.0	36.0	38.0	31.0	38.0
70-71	35.061574850517715	38.0	36.0	38.0	27.0	38.0
72-73	34.75415633659034	38.0	36.0	38.0	25.0	38.0
74-75	34.74238004958437	38.0	36.0	38.0	25.0	38.0
76	33.86955848038501	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	7.0
22	22.0
23	47.0
24	104.0
25	183.0
26	332.0
27	611.0
28	1020.0
29	1578.0
30	2253.0
31	3385.0
32	4764.0
33	6284.0
34	8960.0
35	12725.0
36	18032.0
37	49403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.69884789266443	15.804105293860287	9.209566865976376	30.28747994749891
2	23.894378007875165	19.000656263672163	31.89988333090273	25.20508239754995
3	20.732463176316173	19.641424821350444	30.77512031500656	28.85099168732682
4	15.19706139711244	19.84924165086773	32.43583199649993	32.517864955519904
5	15.033906956394924	30.58917894122794	37.07069418112877	17.30621992124836
6	30.652070876476596	37.24752078168295	16.920665013854457	15.179743327986001
7	29.51454717806621	26.646128044334255	19.22761411696077	24.611710660638764
8	25.86225754703223	35.99606241796704	17.32718389966458	20.814496135336153
9	24.4558480385008	15.669206650138545	17.98435904914686	41.890586262213795
10-11	25.66127679743328	24.85279641242526	26.098330173545282	23.38759661659618
12-13	25.490374799474992	20.23023917164941	25.918769141023773	28.36061688785183
14-15	23.639620096252006	20.830446988478926	21.051935977832873	34.477996937436195
16-17	27.294188420592096	25.865447717660782	21.76015385737203	25.080210004375093
18-19	26.87855476155753	23.676990666472218	22.488879976666183	26.95557459530407
20-21	23.721197316610763	23.419042584220502	22.74546084293423	30.114299256234506
22-23	24.123614554469885	25.894159253317778	22.78693306110544	27.195293131106897
24-25	26.640659180399588	25.777034417383692	22.655224588012253	24.927081814204463
26-27	26.2099861455447	26.358556949103107	23.049438529969375	24.38201837538282
28-29	26.669826454717803	23.624580720431677	23.440918039959165	26.26467478489135
30-31	27.410857517864955	22.812454426133876	25.73465072189004	24.04203733411113
32-33	23.405826163045063	25.92059209566866	23.732590783141315	26.940990958144962
34-35	24.30636575761995	23.228543823829664	28.483210587720574	23.981879830829808
36-37	23.994640513344027	25.114390403966752	26.350353653201108	24.540615429488113
38-39	23.33974405716786	23.792292547761413	26.278802683389234	26.589160711681494
40-41	23.299183316319088	22.578660492926936	26.969702493801957	27.152453696952023
42-43	26.23778620387925	22.632437654951147	26.135700743765494	24.99407539740411
44-45	22.997940061251278	25.346817121190025	27.12647659326236	24.52876622429634
46-47	25.562381507948086	22.611017937873708	24.877406300131252	26.949194254046958
48-49	25.503591220650428	22.27012541927957	24.550641680035	27.675641680035
50-51	22.479765203441737	22.334384570511887	27.55350371882748	27.6323465072189
52-53	21.931967332652764	22.364463322152545	30.410073647367653	25.29349569782704
54-55	21.495369695201983	22.52397185358028	28.188347673909874	27.79231077730786
56-57	24.079863642992564	22.683936123669245	25.27891206066793	27.957288172670264
58-59	21.61158305381362	22.72176243255068	29.865921685868457	25.80073282776725
60-61	23.95635846580137	25.033724660930435	25.063803412571094	25.946113460697095
62-63	20.77028948519761	28.477285985124688	24.58345486364299	26.16896966603471
64-65	21.31945457197025	28.71973895289485	23.792748286422633	26.168058188712262
66-67	21.349989062272133	28.7867325360945	23.244494676972437	26.618783724660933
68-69	21.071532740265422	29.084329881872538	23.53935759078314	26.304779787078896
70-71	21.68404550094794	27.85110106460551	23.522495260317925	26.94235817412863
72-73	22.04772495260318	25.329499052063586	24.11586699722911	28.506908998104123
74-75	22.091931602741724	25.198702056292838	24.41392008166837	28.29544625929707
76	22.59916873268193	24.756635554907394	24.214306548053084	28.42988916435759
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	3.0
15	5.0
16	7.5
17	11.0
18	12.5
19	27.0
20	49.5
21	59.0
22	80.5
23	142.0
24	215.5
25	249.0
26	275.0
27	351.0
28	504.0
29	607.0
30	694.0
31	848.5
32	1146.5
33	1377.0
34	1515.0
35	1862.5
36	2383.0
37	2694.0
38	2922.5
39	3856.0
40	5389.5
41	6562.5
42	6907.0
43	6785.0
44	6467.5
45	6158.0
46	6044.0
47	5823.0
48	5240.5
49	4785.5
50	4692.0
51	4296.0
52	3563.5
53	3031.5
54	2836.0
55	2688.0
56	2358.0
57	2059.5
58	1943.0
59	1798.0
60	1633.5
61	1584.0
62	1554.0
63	1580.0
64	1584.5
65	1638.5
66	1729.0
67	1744.0
68	1771.0
69	1781.0
70	1757.5
71	1751.0
72	1712.5
73	1659.0
74	1538.0
75	1432.0
76	1374.0
77	1195.0
78	948.0
79	822.0
80	755.0
81	585.0
82	397.0
83	312.0
84	254.0
85	147.5
86	83.5
87	68.0
88	51.0
89	25.0
90	12.5
91	4.5
92	0.0
93	2.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	109712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.6046375966166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.613081105920195	28.854637596616595
2	20.613464042276174	19.625929706868895
3	8.418855786168338	12.023297360361674
4	3.695335835184192	7.036604929269359
5	1.8917055985295244	4.502697972874435
6	1.1890173853105614	3.396164503427155
7	0.7677873937351612	2.558516844100919
8	0.618442214903883	2.3552574011958582
9	0.40782721911618286	1.7473020271255653
>10	1.7691659646166806	12.449868747265567
>50	0.003829363559776365	0.14401341694618638
>100	0.009573408899440913	1.1785401779203735
>500	0.0	0.0
>1k	0.0019146817798881826	4.127169316027417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	4528	4.127169316027417	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG	465	0.42383695493656115	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC	299	0.27253171941082105	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTACCTCGTATGCCGT	245	0.2233119439988333	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTAACTCGTATGCCGT	163	0.14857080355840746	No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT	121	0.11028875601575033	RNA PCR Primer, Index 45 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0027344319673326526	0.0
2	0.0	0.0	0.0	0.0027344319673326526	0.0
3	0.0	0.0	0.0	0.0027344319673326526	0.0
4	0.0	0.0	0.0	0.00364590928977687	0.0
5	0.0	0.0	0.0	0.005468863934665305	0.0
6	0.0	0.0	0.0	0.006380341257109523	0.0
7	0.0	0.0	0.0	0.006380341257109523	0.0
8	0.0	0.0	0.0	0.006380341257109523	0.0
9	0.0	0.0	0.0	0.010026250546886394	0.0
10	0.0	0.0	0.0	0.01093772786933061	0.0
11	0.0	0.0	0.0	0.01093772786933061	0.0
12	0.0	0.0	0.0	0.01093772786933061	0.0
13	0.0	0.0	0.0	0.011849205191774828	0.0
14	0.0	0.0	0.0	0.012760682514219046	0.0
15	0.0	0.0	0.0	0.012760682514219046	0.0
16	0.0	0.0	0.0	0.013672159836663264	0.0
17	0.0	0.0	0.0	0.013672159836663264	0.0
18	0.0	0.0	0.0	0.01458363715910748	0.0
19	0.0	0.0	0.0	0.01458363715910748	0.0
20	0.0	0.0	0.0	0.017318069126440135	0.0
21	0.0	0.0	0.0	0.017318069126440135	0.0
22	0.0	0.0	0.0	0.018229546448884353	0.0
23	0.0	0.0	0.0	0.02005250109377279	0.0
24	0.0	0.0	0.0	0.02005250109377279	0.0
25	0.0	0.0	0.0	0.020963978416217003	0.0
26	0.0	0.0	0.0	0.02187545573866122	0.0
27	0.0	0.0	0.0	0.03737057022021292	0.0
28	0.0	0.0	0.0	0.05377716202420884	0.0
29	0.0	0.0	0.0	0.07474114044042585	0.0
30	0.0	0.0	0.0	0.10664284672597346	0.0
31	0.0	0.0	0.0	0.1348986437217442	0.0
32	0.0	0.0	0.0	0.17409216858684556	0.0
33	0.0	0.0	0.0	0.23607262651305236	0.0
34	0.0	0.0	0.0	0.3208400175003646	0.0
35	0.0	0.0	0.0	0.4211025229692285	0.0
36	0.0	0.0	0.0	0.603397987458072	0.0
37	0.0	0.0	0.0	0.8859559574157795	0.0
38	0.0	0.0	0.0	1.2159107481405862	0.0
39	0.0	0.0	0.0	1.562272130669389	0.0
40	0.0	0.0	0.0	1.9450926060959604	0.0
41	0.0	0.0	0.0	2.3853361528365173	0.0
42	0.0	0.0	0.0	2.852924019250401	0.0
43	0.0	0.0	0.0	3.37793495697827	0.0
44	0.0	0.0	0.0	4.01323465072189	0.0
45	0.0	0.0	0.0	4.64853434446551	0.0
46	0.0	0.0	0.0	5.313001312527344	0.0
47	0.0	0.0	0.0	6.106898060376258	0.0
48	0.0	0.0	0.0	6.822407758494968	0.0
49	0.0	0.0	0.0	7.472291089397696	0.0
50	0.0	0.0	0.0	8.054725098439551	0.0
51	0.0	0.0	0.0	8.677264109668952	0.0
52	0.0	0.0	0.0	9.257875164065918	0.0
53	0.0	0.0	0.0	9.846689514364883	0.0
54	0.0	0.0	0.0	10.389018521219192	0.0
55	0.0	0.0	0.0	11.099059355403238	0.0
56	0.0	0.0	0.0	11.789047688493511	0.0
57	0.0	0.0	0.0	12.439842496718681	0.0
58	0.0	0.0	0.0	13.087902872976521	0.0
59	0.0	0.0	0.0	13.684920519177483	0.0
60	0.0	0.0	0.0	14.365794079043313	0.0
61	0.0	0.0	0.0	14.966457634534052	0.0
62	0.0	0.0	0.0	15.51243255067814	0.0
63	0.0	0.0	0.0	16.10671576491177	0.0
64	0.0	0.0	0.0	16.690061251276067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATGA	15	0.0022195294	70.0	55
TAACTCG	15	0.0022195294	70.0	36
TCGTATG	645	0.0	59.689922	40
CTCGTAT	630	0.0	59.444443	39
TCTCGTA	560	0.0	59.375	38
CGTATGC	650	0.0	59.23077	41
TATGCCG	650	0.0	59.23077	43
GTATGCC	670	0.0	57.46269	42
ATCTCGT	580	0.0	57.327587	37
CTGCTTG	635	0.0	56.771656	54
TCTTCTG	605	0.0	56.694214	50
GCCGTCT	630	0.0	56.666664	46
ATGCCGT	670	0.0	56.41791	44
CTTGAAA	640	0.0	56.328125	57
TATCTCG	595	0.0	55.88235	36
CATTTAT	615	0.0	55.772358	32
TGAAAAA	655	0.0	55.572517	59
TTATCTC	605	0.0	55.53719	35
TTGAAAA	650	0.0	55.46154	58
CGTCTTC	640	0.0	55.234375	48
>>END_MODULE