FastQCFastQC Report
Wed 25 May 2016
SRR1781175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157738
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGCG252.3846942E-456.00000431
GTAGGCA200.006940976352.533
TACTCGG200.006940976352.531
GCAGAGT200.006940976352.548
AGTCATC305.863901E-446.6666648
TTGCGTT305.863901E-446.66666433
GGTAGAT305.863901E-446.66666418
TGGAGCA350.001252477640.039
TGGACTA350.001252477640.042
AAAGTAG350.001252477640.021
GCTTAAT350.001252477640.013
GATGCAG459.1658294E-538.8888945
TATAGAT501.7038124E-435.0000045
ATCTAAT400.002413130835.01
AGCTGAT400.002413130835.026
TGCAGAG400.002413130835.047
GCAGTAG552.9818396E-431.8181846
GGGCTTA450.00429730631.1111136
ATAGCCG450.00429730631.11111311
GTGCTAG450.00429730631.11111327