FastQCFastQC Report
Wed 25 May 2016
SRR1781175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146988
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT44363.0179334367431356No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4330.29458187062889485No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3050.20749993196723543No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT2650.18028682613546684No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT1610.10953275097286853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA252.3841574E-455.99999648
GCCGTCT7300.052.73972346
TTGCTCT200.006940038452.54
ACGATAG200.006940038452.549
TGATTCG200.006940038452.551
GTGTCGA200.006940038452.51
CCGTCTT7400.052.02702747
GTCTTCT7150.051.8881149
CGTCTTC7300.051.7808248
TATGCCG7600.051.57894543
CGTATGC7550.051.45695541
TCGTATG7450.051.20805740
CTTCTGC7250.051.17241351
ATGCCGT7600.051.1184244
TCTTCTG7300.050.82191550
CTTGAAA7300.050.82191557
CTCGTAT7600.050.6578939
TGCCGTC7650.050.32679745
GTATGCC7900.049.62025542
ATCTCGT7000.049.50000437
Produced by FastQC (version 0.11.5)