FastQCFastQC Report
Wed 25 May 2016
SRR1781174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506577
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATT200.00694980452.519
GCTCGAT200.00694980452.524
CACGCTA404.5911496E-543.7523
ACCCGAC400.002418160235.036
CCGACCA552.991109E-431.81818438
GGACGAT500.00720662327.99999818
TTCGGTC500.00720662327.99999828
TACCACG657.95316E-426.92307720
CGTGCGA657.95316E-426.92307710
GCTCTAC657.95316E-426.9230771
CGAGGAT657.95316E-426.92307724
ACGCTAC657.95316E-426.92307724
CTACTAG1757.2759576E-1226.0000021
CGAGTGC700.001225607825.045
ACTTCGG700.001225607825.034
CGCTACT700.001225607825.025
ACGTCCG700.001225607825.043
CCACGCT700.001225607825.022
CGCAAAA851.3076082E-424.70588322
CATCGCC1301.6039303E-724.23076811
Produced by FastQC (version 0.11.5)