##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781174_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506577
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177558396847864	34.0	32.0	34.0	21.0	34.0
2	31.365484417966073	34.0	33.0	34.0	23.0	34.0
3	31.445993402779834	34.0	33.0	34.0	23.0	34.0
4	31.539667217422032	34.0	33.0	34.0	23.0	34.0
5	31.608965665634248	34.0	33.0	34.0	23.0	34.0
6	34.49350246852897	37.0	34.0	38.0	26.0	38.0
7	34.44492150255539	37.0	34.0	38.0	25.0	38.0
8	34.22727640615346	37.0	34.0	38.0	24.0	38.0
9	34.49978186929134	37.0	34.0	38.0	26.0	38.0
10-11	34.36989736999509	37.0	34.0	38.0	25.0	38.0
12-13	34.34085834927366	37.0	34.0	38.0	25.0	38.0
14-15	34.22111248635449	37.0	34.0	38.0	24.0	38.0
16-17	34.188777816600435	37.0	34.0	38.0	24.0	38.0
18-19	34.25290824494598	37.0	34.0	38.0	24.0	38.0
20-21	34.174037707989115	37.0	34.0	38.0	24.0	38.0
22-23	34.24936880276839	37.0	34.0	38.0	24.5	38.0
24-25	34.251194783813716	37.0	34.0	38.0	24.5	38.0
26-27	34.159848354741726	37.0	34.0	38.0	24.0	38.0
28-29	34.26026842908384	37.0	34.0	38.0	24.0	38.0
30-31	34.34273269414126	37.0	34.0	38.0	25.0	38.0
32-33	34.286862609238085	37.0	34.0	38.0	24.5	38.0
34-35	34.32310487842915	37.0	34.0	38.0	24.5	38.0
36-37	34.16366909670198	37.0	34.0	38.0	24.0	38.0
38-39	34.182898157634476	37.0	34.0	38.0	24.0	38.0
40-41	34.17860364761921	37.0	34.0	38.0	24.0	38.0
42-43	34.238231305408654	37.0	34.0	38.0	24.0	38.0
44-45	34.26092479524337	37.0	34.0	38.0	24.0	38.0
46-47	34.253214220148166	37.0	34.0	38.0	24.0	38.0
48-49	34.2310714856774	37.0	34.0	38.0	24.0	38.0
50-51	34.0891651219854	37.0	34.0	38.0	24.0	38.0
52-53	34.16320421179801	37.0	34.0	38.0	24.0	38.0
54-55	34.124384841791084	37.0	34.0	38.0	24.0	38.0
56-57	34.101698261073835	37.0	34.0	38.0	24.0	38.0
58-59	34.10232600374671	37.0	34.0	38.0	24.0	38.0
60-61	34.0805593226696	37.0	34.0	38.0	24.0	38.0
62-63	34.05465111917833	37.0	34.0	38.0	24.0	38.0
64-65	34.087689531897425	37.0	34.0	38.0	24.0	38.0
66-67	33.994196341326194	37.0	34.0	38.0	24.0	38.0
68-69	33.781574173324096	37.0	34.0	38.0	23.5	38.0
70-71	33.94318731407071	37.0	34.0	38.0	24.0	38.0
72-73	33.92566085708589	37.0	34.0	38.0	24.0	38.0
74-75	33.93172607520673	37.0	34.0	38.0	24.0	38.0
76	32.68924763658832	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	23.0
14	114.0
15	285.0
16	645.0
17	1252.0
18	2081.0
19	2625.0
20	3306.0
21	3991.0
22	4626.0
23	5513.0
24	6500.0
25	7644.0
26	8949.0
27	10754.0
28	12472.0
29	14769.0
30	17422.0
31	20481.0
32	25048.0
33	29553.0
34	37880.0
35	49887.0
36	75281.0
37	165472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.680654668490675	17.518560850571582	9.05745819490423	24.743326286033515
2	24.15684091460923	20.875404923634512	30.685562115927095	24.282192045829163
3	19.71388357544855	22.049757489976844	30.43466245013098	27.801696484443628
4	13.845476600793166	16.794090533127246	37.165722091607	32.19471077447259
5	13.652416118378843	33.09585709576234	36.427433539225035	16.82429324663378
6	28.92610600165424	37.57572886254212	19.35145101337013	14.14671412243351
7	28.79878083687179	30.513623792631723	21.970598744119847	18.71699662637664
8	25.956962120269967	34.8450482355101	20.104347414114734	19.093642230105196
9	24.9430984825604	17.355703081663794	21.73233289312385	35.968865542651955
10-11	24.875093026331633	24.89355024014118	28.1563316139501	22.07502511957708
12-13	25.283027062026108	23.2633933242133	27.498583630918894	23.9549959828417
14-15	23.146135730599692	24.173422796534386	24.920495798269563	27.75994567459636
16-17	23.17209427194681	27.929021649226083	25.455261490355856	23.44362258847125
18-19	22.434003122921094	27.473414702996777	27.22666050768195	22.865921666400173
20-21	22.670788448745206	27.455648400934113	27.03725198735829	22.836311162962392
22-23	22.775807034271196	26.9508880189981	27.37293639466458	22.900368552066123
24-25	22.715993817326883	27.027677924580075	27.090945700258796	23.16538255783425
26-27	22.128324025765085	27.21195395764119	27.522765542059744	23.136956474533978
28-29	22.270750547300803	27.22281114223504	27.995151773570452	22.511286536893703
30-31	22.70336000252676	27.195075970681653	27.498682332597017	22.602881694194565
32-33	22.16869301211859	27.560469583103853	27.52799673100042	22.742840673777135
34-35	22.813214970280924	26.75654441476814	27.728065032561684	22.702175582389252
36-37	22.819531877680983	26.890285188628777	27.160826488371953	23.129356445318283
38-39	22.179945003424947	27.42919635119636	27.748891086646253	22.641967558732436
40-41	22.37468341436741	26.795235472593504	28.014102495770633	22.81597861726845
42-43	22.334117024657655	26.653993371195295	27.860226579572306	23.151663024574745
44-45	21.879200990175235	26.717458550230273	27.945110022760606	23.458230436833887
46-47	22.105227833083617	26.44188346490267	28.18100703348158	23.271881668532128
48-49	21.541739952662674	26.39312483590846	28.822765344656393	23.242369866772474
50-51	21.793330530205672	26.401711881905417	28.457569135590443	23.347388452298464
52-53	21.446591534949278	26.20756568103171	28.899950056950868	23.445892727068145
54-55	20.902153078406638	26.58667882671341	28.92126961942607	23.58989847545388
56-57	20.83181990461451	26.718300906478	28.685429234705158	23.76444995420233
58-59	20.953420612109536	26.315893370392594	28.814432582672687	23.91625343482518
60-61	20.79243785730078	26.41429913774044	28.50875288209469	24.28451012286409
62-63	20.51775054483434	26.282334733583905	28.946594390575154	24.2533203310066
64-65	21.040475664066204	26.273155459397994	28.52582830611794	24.160540570417865
66-67	21.015504090205617	26.25272417169388	28.118189570765296	24.613582167335206
68-69	20.779804017561037	26.195674489119103	27.94802754177063	25.07649395154922
70-71	21.464795015950223	26.22064606297969	27.667615520672122	24.646943400397966
72-73	21.221988408452038	25.98632781655665	27.78388632702694	25.007797447964375
74-75	21.38494520072013	26.348958497836456	27.447609045829253	24.818487255614162
76	21.557433519484697	26.378418285867696	27.151449828949993	24.912698365697615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	1.0
8	0.0
9	0.5
10	1.5
11	2.0
12	2.0
13	4.0
14	11.0
15	37.5
16	83.0
17	107.0
18	177.0
19	339.5
20	567.0
21	702.0
22	899.0
23	1390.0
24	2027.5
25	2371.0
26	2842.0
27	3805.5
28	4803.5
29	5309.0
30	6153.5
31	7676.0
32	9651.5
33	10949.0
34	12043.5
35	14416.5
36	17144.0
37	18593.0
38	19841.5
39	22839.0
40	26130.0
41	28381.0
42	29090.0
43	29703.0
44	30221.0
45	30161.5
46	30197.0
47	29754.0
48	27652.5
49	24900.5
50	23807.0
51	22398.0
52	19195.5
53	16245.5
54	15089.0
55	14028.0
56	12170.0
57	10515.5
58	9658.0
59	8790.5
60	7172.5
61	5892.5
62	5363.0
63	4951.5
64	4185.5
65	3591.5
66	3112.0
67	2872.0
68	2692.0
69	2424.5
70	2304.0
71	2271.0
72	2194.0
73	1989.0
74	1783.5
75	1706.0
76	1558.0
77	1333.5
78	1125.5
79	994.0
80	897.5
81	714.0
82	537.5
83	448.0
84	391.5
85	281.5
86	176.5
87	125.0
88	96.5
89	60.0
90	43.0
91	24.0
92	14.0
93	13.5
94	10.0
95	5.0
96	3.0
97	2.0
98	1.0
99	5.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.97403356251863E-4
58-59	1.97403356251863E-4
60-61	1.97403356251863E-4
62-63	1.97403356251863E-4
64-65	1.97403356251863E-4
66-67	1.97403356251863E-4
68-69	1.97403356251863E-4
70-71	1.97403356251863E-4
72-73	1.97403356251863E-4
74-75	1.97403356251863E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	506577.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.51147275107478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21347767282651	40.641879708465304
2	15.32413508685175	17.0133061461512
3	5.562113384574522	9.262833167585907
4	1.9778524954365024	4.391740196242746
5	0.8292055263142842	2.3015209989518004
6	0.47217397910799846	1.5726643785012127
7	0.33812676524866275	1.313894030085714
8	0.2704850910681274	1.2012020609920266
9	0.22123620270684743	1.1053032694301177
>10	1.7035849081298504	17.872931497639033
>50	0.0822450783627583	2.9668105103934463
>100	0.00536380937214557	0.3559140355614975
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.94806712503726E-4	0.0
2	0.0	0.0	0.0	3.94806712503726E-4	0.0
3	0.0	0.0	0.0	5.92210068755589E-4	0.0
4	0.0	0.0	0.0	0.0017766302062667669	0.0
5	0.0	0.0	0.0	0.00197403356251863	0.0
6	0.0	0.0	0.0	0.00197403356251863	0.0
7	0.0	0.0	0.0	0.00197403356251863	0.0
8	0.0	0.0	0.0	0.00197403356251863	0.0
9	0.0	0.0	0.0	0.002171436918770493	0.0
10	0.0	0.0	0.0	0.002171436918770493	0.0
11	0.0	0.0	0.0	0.002171436918770493	0.0
12	0.0	0.0	0.0	0.002171436918770493	0.0
13	0.0	0.0	0.0	0.002566243631274219	0.0
14	0.0	0.0	0.0	0.002763646987526082	0.0
15	0.0	0.0	1.97403356251863E-4	0.002763646987526082	0.0
16	0.0	0.0	1.97403356251863E-4	0.002763646987526082	0.0
17	0.0	0.0	1.97403356251863E-4	0.003158453700029808	0.0
18	0.0	0.0	1.97403356251863E-4	0.003355857056281671	0.0
19	0.0	0.0	1.97403356251863E-4	0.003750663768785397	0.0
20	0.0	0.0	1.97403356251863E-4	0.003750663768785397	0.0
21	0.0	0.0	1.97403356251863E-4	0.004342873837540986	0.0
22	0.0	0.0	1.97403356251863E-4	0.005724697331304027	0.0
23	0.0	0.0	1.97403356251863E-4	0.005922100687555889	0.0
24	0.0	0.0	1.97403356251863E-4	0.006909117468815205	0.0
25	0.0	0.0	1.97403356251863E-4	0.007501327537570794	0.0
26	0.0	0.0	1.97403356251863E-4	0.008093537606326383	0.0
27	0.0	0.0	1.97403356251863E-4	0.011449394662608053	0.0
28	0.0	0.0	1.97403356251863E-4	0.01855591548767512	0.0
29	0.0	0.0	1.97403356251863E-4	0.02901829336902386	0.0
30	0.0	0.0	1.97403356251863E-4	0.03987547796287633	0.0
31	0.0	0.0	1.97403356251863E-4	0.052509292762995555	0.0
32	0.0	0.0	1.97403356251863E-4	0.0683015612631446	0.0
33	0.0	0.0	1.97403356251863E-4	0.09179256065711629	0.0
34	0.0	0.0	1.97403356251863E-4	0.12258748423240692	0.0
35	0.0	0.0	1.97403356251863E-4	0.17055649980160964	0.0
36	0.0	0.0	1.97403356251863E-4	0.2501100523711104	0.0
37	0.0	0.0	1.97403356251863E-4	0.3744741668097841	0.0
38	0.0	0.0	1.97403356251863E-4	0.5472021035301642	0.0
39	0.0	0.0	1.97403356251863E-4	0.7724393330135398	0.0
40	0.0	0.0	1.97403356251863E-4	1.0320247464847396	0.0
41	0.0	0.0	1.97403356251863E-4	1.3162855794874224	0.0
42	0.0	0.0	1.97403356251863E-4	1.6623336629969383	0.0
43	0.0	0.0	1.97403356251863E-4	2.0482572244693302	0.0
44	0.0	0.0	1.97403356251863E-4	2.523407102967565	0.0
45	0.0	0.0	1.97403356251863E-4	3.0783079373915516	0.0
46	0.0	0.0	1.97403356251863E-4	3.6946012156098678	0.0
47	0.0	0.0	1.97403356251863E-4	4.321949081778288	0.0
48	0.0	0.0	1.97403356251863E-4	4.95679827548428	0.0
49	0.0	0.0	1.97403356251863E-4	5.559075915408714	0.0
50	0.0	0.0	1.97403356251863E-4	6.105883212226375	0.0
51	0.0	0.0	1.97403356251863E-4	6.643017744587693	0.0
52	0.0	0.0	1.97403356251863E-4	7.171466529273931	0.0
53	0.0	0.0	1.97403356251863E-4	7.698138683753901	0.0
54	0.0	0.0	1.97403356251863E-4	8.282650021615668	0.0
55	0.0	0.0	1.97403356251863E-4	8.903286173671525	0.0
56	0.0	0.0	1.97403356251863E-4	9.520961275383604	0.0
57	0.0	0.0	1.97403356251863E-4	10.119883058251757	0.0
58	0.0	0.0	1.97403356251863E-4	10.724332135094961	0.0
59	0.0	0.0	1.97403356251863E-4	11.302526565556668	0.0
60	0.0	0.0	1.97403356251863E-4	11.86334950066821	0.0
61	0.0	0.0	1.97403356251863E-4	12.371663143016757	0.0
62	0.0	0.0	1.97403356251863E-4	12.89951971763424	0.0
63	0.0	0.0	1.97403356251863E-4	13.440799720476848	0.0
64	0.0	0.0	1.97403356251863E-4	13.955627673581706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATT	20	0.006949804	52.5	19
GCTCGAT	20	0.006949804	52.5	24
CACGCTA	40	4.5911496E-5	43.75	23
ACCCGAC	40	0.0024181602	35.0	36
CCGACCA	55	2.991109E-4	31.818184	38
GGACGAT	50	0.007206623	27.999998	18
TTCGGTC	50	0.007206623	27.999998	28
TACCACG	65	7.95316E-4	26.923077	20
CGTGCGA	65	7.95316E-4	26.923077	10
GCTCTAC	65	7.95316E-4	26.923077	1
CGAGGAT	65	7.95316E-4	26.923077	24
ACGCTAC	65	7.95316E-4	26.923077	24
CTACTAG	175	7.2759576E-12	26.000002	1
CGAGTGC	70	0.0012256078	25.0	45
ACTTCGG	70	0.0012256078	25.0	34
CGCTACT	70	0.0012256078	25.0	25
ACGTCCG	70	0.0012256078	25.0	43
CCACGCT	70	0.0012256078	25.0	22
CGCAAAA	85	1.3076082E-4	24.705883	22
CATCGCC	130	1.6039303E-7	24.230768	11
>>END_MODULE