FastQCFastQC Report
Wed 25 May 2016
SRR1781174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451736
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT20610.45623992774540884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3350.053.28358540
CTCGTAT3400.052.50000439
ATCTCGT3000.052.537
TATGCCG3500.051.043
TCTCGTA3100.050.80645438
CGTATGC3550.050.28168541
CCGTCTT3550.050.28168547
CGTCTTC3650.048.9041148
ATGCCGT3600.048.61110744
GCCGTCT3750.046.66666846
TGCCGTC3800.046.05262845
GTATGCC3900.045.7692342
CGCAGAT3450.045.65217232
TCGCAGA3850.045.45454431
GATCTCG3800.040.52631436
AGATCTC4250.037.05882335
CCTCGCA4650.036.8817229
CTCGCAG4750.036.84210630
CTTGAAA4650.036.12903257
CAGATCT4400.035.79545234
Produced by FastQC (version 0.11.5)