##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781173_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17339
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.341138473960434	31.0	12.0	34.0	12.0	34.0
2	25.971624661168462	32.0	12.0	34.0	12.0	34.0
3	25.721667916258145	32.0	11.0	34.0	11.0	34.0
4	25.94884364726916	32.0	11.0	34.0	11.0	34.0
5	25.828709844858412	32.0	11.0	34.0	11.0	34.0
6	27.240902012803506	33.0	11.0	38.0	11.0	38.0
7	27.391026010727263	32.0	12.0	38.0	12.0	38.0
8	26.957725359017246	32.0	11.0	37.0	11.0	38.0
9	27.815617971047928	34.0	12.0	38.0	11.0	38.0
10-11	27.221350712267142	33.5	11.0	38.0	11.0	38.0
12-13	27.235567218409365	32.5	11.0	38.0	11.0	38.0
14-15	27.12682392294827	31.0	11.0	37.5	11.0	38.0
16-17	26.95351519695484	31.0	11.0	37.0	11.0	38.0
18-19	27.09040313743584	31.0	11.0	37.0	11.0	38.0
20-21	26.93480016148567	31.0	11.0	37.0	11.0	38.0
22-23	26.970355845204452	31.0	11.0	37.0	11.0	38.0
24-25	26.982697964127112	31.0	11.0	37.0	11.0	38.0
26-27	26.891804602341544	31.0	11.0	37.0	11.0	38.0
28-29	26.895120825883843	31.0	11.0	37.0	11.0	38.0
30-31	26.944056750677664	31.0	11.0	37.0	11.0	38.0
32-33	26.834361843243556	31.0	11.0	37.0	11.0	38.0
34-35	26.916113962742948	31.0	11.0	37.0	11.0	38.0
36-37	26.850654593690525	31.0	11.0	37.0	11.0	38.0
38-39	26.795518772708924	31.0	11.0	37.0	11.0	38.0
40-41	26.835140434857834	31.0	11.0	37.0	11.0	38.0
42-43	26.876722994405675	31.0	11.0	37.0	11.0	38.0
44-45	26.992242920583656	31.0	11.0	37.0	11.0	38.0
46-47	26.863256243151277	31.0	11.0	37.0	11.0	38.0
48-49	26.751312071053697	31.0	11.0	37.0	11.0	38.0
50-51	26.690870292404405	31.0	11.0	37.0	11.0	38.0
52-53	26.698656208547206	31.0	11.0	37.0	11.0	38.0
54-55	26.73259703558452	31.0	11.0	37.0	11.0	38.0
56-57	26.678730030566932	31.0	11.0	37.0	11.0	38.0
58-59	26.69897341253821	31.0	11.0	37.0	11.0	38.0
60-61	26.666906972720458	31.0	11.0	37.0	11.0	38.0
62-63	26.54931080223773	31.0	11.0	37.0	11.0	38.0
64-65	26.604879174116157	31.0	11.0	37.0	11.0	38.0
66-67	26.48982063556145	31.0	11.0	37.0	11.0	38.0
68-69	26.158861526039566	29.0	11.0	37.0	11.0	38.0
70-71	26.32683545763885	31.0	11.0	37.0	11.0	38.0
72-73	26.184209008593342	30.0	11.0	37.0	11.0	38.0
74-75	26.129736432320204	29.5	11.0	37.0	11.0	38.0
76	25.032124113270662	27.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	41.0
14	111.0
15	340.0
16	742.0
17	1373.0
18	1604.0
19	1523.0
20	1097.0
21	740.0
22	434.0
23	387.0
24	301.0
25	272.0
26	266.0
27	254.0
28	271.0
29	329.0
30	351.0
31	403.0
32	466.0
33	565.0
34	751.0
35	865.0
36	1355.0
37	2497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.45187150354692	19.805063729165465	12.936155487629044	31.806909279658573
2	19.4763250475806	17.515427648653326	34.811696176250074	28.196551127516006
3	20.10496568429552	17.492358267489475	29.119326374070013	33.28334967414499
4	17.01366860833958	15.294999711632734	29.367322221581404	38.32400945844628
5	16.137032124113272	25.820404867639425	32.26252955764461	25.780033450602684
6	30.809158544322045	26.299094526789318	18.461272276371187	24.430474652517447
7	26.73164542361151	22.07739777380472	22.78678124459311	28.40417555799066
8	22.42343849126247	27.815906338312473	23.42695657188996	26.333698598535094
9	20.329892150643058	14.729799873118404	23.305842320779746	41.63446565545879
10-11	24.274756329661457	20.531749235826748	27.32856566122614	27.86492877328566
12-13	22.97710363919488	18.85345175615664	26.538439356364265	31.631005248284218
14-15	22.28502220427937	20.329892150643058	23.749927908183864	33.6351577368937
16-17	22.77236288136571	21.644846876982523	24.0527135359594	31.530076705692373
18-19	21.94474883211258	20.970067477939907	26.016494607532152	31.068689082415364
20-21	23.057846473268352	21.647730549628005	25.258088701770575	30.036334275333065
22-23	23.571140204164024	20.612492069900227	24.701539881192687	31.114827844743065
24-25	22.518599688563352	21.105600092277523	24.66405213680143	31.711748082357687
26-27	22.397485437453142	21.549685679681645	25.58682738335544	30.466001499509776
28-29	22.25618547782456	21.347828594497955	25.728127342984024	30.667858584693462
30-31	22.455158890362767	21.12001845550493	25.269623392352504	31.155199261779803
32-33	22.319626276025144	21.610242805236748	25.185996885633543	30.88413403310456
34-35	22.420554818616992	20.76244304746525	25.98477420843186	30.8322279254859
36-37	23.153007670569238	19.68394947805525	25.837706903512313	31.3253359478632
38-39	22.50706499798143	19.18507411038699	27.354518715035468	30.95334217659611
40-41	22.132187554068864	19.07261087721322	28.46184901090028	30.33335255781764
42-43	20.551934944345117	18.87363746467501	28.6435203875656	31.930907203414264
44-45	19.894457581175384	19.216794509487283	30.042101620624027	30.846646288713302
46-47	21.267085760424475	20.254916661860545	28.4589653382548	30.019032239460174
48-49	20.433704365880384	20.94123075148509	28.202318472806965	30.422746409827557
50-51	20.549051271699636	21.25555106984255	27.207451410115922	30.987946248341885
52-53	19.36674548705231	21.99377126708576	27.925485898840762	30.713997347021166
54-55	19.76180863948325	20.748024684237844	27.46698194820924	32.02318472806967
56-57	20.012688159640117	21.950516177403543	27.363169732971915	30.67362592998443
58-59	20.64997981429148	20.58365534344541	27.02289636080512	31.743468481457988
60-61	20.113616702231962	20.28952073360632	27.39489013207221	32.20197243208951
62-63	20.551934944345117	20.410634984716538	27.19880039217948	31.838629678758867
64-65	20.89509198915739	19.810831074456427	26.474998558163676	32.81907837822251
66-67	20.667281850164372	21.665032585500892	25.278274410288944	32.389411154045796
68-69	21.382432666243727	22.919430186285254	24.50256646865448	31.19557067881654
70-71	20.675932868100812	22.720456773747046	25.670453890074395	30.933156468077744
72-73	20.439471711171347	24.764980679393275	25.232135647961247	29.563411961474134
74-75	20.540400253763195	23.60286060326432	25.552223311609666	30.30451583136282
76	20.618259415191186	21.898610069784876	26.61629851779226	30.866831997231674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	2.0
16	2.0
17	3.0
18	8.5
19	14.0
20	11.5
21	9.0
22	20.5
23	35.5
24	48.5
25	58.0
26	73.5
27	105.5
28	140.0
29	158.0
30	159.0
31	184.0
32	241.0
33	274.0
34	319.0
35	373.5
36	424.5
37	466.0
38	501.0
39	556.0
40	623.5
41	655.0
42	639.0
43	648.5
44	658.5
45	666.0
46	673.0
47	645.0
48	639.0
49	598.5
50	536.0
51	541.0
52	501.0
53	455.5
54	455.0
55	441.0
56	436.5
57	434.0
58	422.0
59	412.0
60	405.5
61	386.5
62	364.0
63	366.5
64	351.0
65	355.0
66	396.5
67	416.0
68	391.5
69	378.5
70	405.0
71	420.0
72	391.0
73	361.0
74	331.5
75	303.0
76	290.5
77	253.0
78	193.5
79	159.0
80	153.5
81	124.5
82	87.0
83	73.0
84	63.5
85	40.5
86	16.5
87	6.0
88	10.0
89	8.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	17339.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.98437049426148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.13357962278825	81.09464213622469
2	6.332231512087628	11.269392698540862
3	1.8212457061377925	4.861872080281446
4	0.5185041156264177	1.8455504931080224
5	0.14906993324259513	0.6632447084606956
6	0.02592520578132089	0.13841628698310168
7	0.012962602890660445	0.08074283407347597
8	0.006481301445330222	0.04613876232770056
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.00576734529096257	0.0
5	0.0	0.0	0.0	0.00576734529096257	0.0
6	0.0	0.0	0.0	0.00576734529096257	0.0
7	0.0	0.0	0.0	0.00576734529096257	0.0
8	0.0	0.0	0.0	0.00576734529096257	0.0
9	0.0	0.0	0.0	0.00576734529096257	0.0
10	0.0	0.0	0.0	0.00576734529096257	0.0
11	0.0	0.0	0.0	0.00576734529096257	0.0
12	0.0	0.0	0.0	0.01153469058192514	0.0
13	0.0	0.0	0.0	0.01153469058192514	0.0
14	0.0	0.0	0.0	0.01153469058192514	0.0
15	0.0	0.0	0.0	0.01730203587288771	0.0
16	0.0	0.0	0.0	0.01730203587288771	0.0
17	0.0	0.0	0.0	0.01730203587288771	0.0
18	0.0	0.0	0.0	0.01730203587288771	0.0
19	0.0	0.0	0.0	0.02306938116385028	0.0
20	0.0	0.0	0.0	0.04613876232770056	0.0
21	0.0	0.0	0.0	0.05190610761866313	0.0
22	0.0	0.0	0.0	0.05190610761866313	0.0
23	0.0	0.0	0.0	0.05190610761866313	0.0
24	0.0	0.0	0.0	0.06920814349155084	0.0
25	0.0	0.0	0.0	0.06920814349155084	0.0
26	0.0	0.0	0.0	0.07497548878251341	0.0
27	0.0	0.0	0.0	0.08074283407347597	0.0
28	0.0	0.0	0.0	0.1153469058192514	0.0
29	0.0	0.0	0.0	0.19608973989272738	0.0
30	0.0	0.0	0.0	0.23646115692946537	0.0
31	0.0	0.0	0.0	0.2479958475113905	0.0
32	0.0	0.0	0.0	0.28259991925716593	0.0
33	0.0	0.0	0.0	0.3518080627487168	0.0
34	0.0	0.0	0.0	0.49022434973181844	0.0
35	0.0	0.0	0.0	0.6401753272968452	0.0
36	0.0	0.0	0.0	0.8074283407347598	0.0
37	0.0	0.0	0.0	1.0842609147009632	0.0
38	0.0	0.0	0.0	1.4187669415767923	0.0
39	0.0	0.0	0.0	1.7763423496164716	0.0
40	0.0	0.0	0.0	2.1569871388200013	0.0
41	0.0	0.0	0.0	2.5895380356421938	0.0
42	0.0	0.0	0.0	2.9471134436818733	0.0
43	0.0	0.0	0.0	3.4142684122498412	0.0
44	0.0	0.0	0.0	3.9390968337274352	0.0
45	0.0	0.0	0.0	4.377415075840591	0.0
46	0.0	0.0	0.0	4.902243497318184	0.0
47	0.0	0.0	0.0	5.305957667685564	0.0
48	0.0	0.0	0.0	5.657765730434281	0.0
49	0.0	0.0	0.0	6.1364553895841745	0.0
50	0.0	0.0	0.0	6.522867524078666	0.0
51	0.0	0.0	0.0	6.903512313282196	0.0
52	0.0	0.0	0.0	7.214948958994175	0.0
53	0.0	0.0	0.0	7.5321529499971165	0.0
54	0.0	0.0	0.0	7.889728358036796	0.0
55	0.0	0.0	0.0	8.218467039621663	0.0
56	0.0	0.0	0.0	8.524136340042679	0.0
57	0.0	0.0	0.0	8.766364842263107	0.0
58	0.0	0.0	0.0	9.135474940884711	0.0
59	0.0	0.0	0.0	9.435376896014764	0.0
60	0.0	0.0	0.0	9.76411557759963	0.0
61	0.0	0.0	0.0	10.035180806274871	0.0
62	0.0	0.0	0.0	10.30047868965915	0.0
63	0.0	0.0	0.0	10.508103120133802	0.0
64	0.0	0.0	0.0	10.877213218755406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTCC	15	0.0021879463	70.0	19
>>END_MODULE