FastQCFastQC Report
Wed 25 May 2016
SRR1781173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15985
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT506331.67344385361276No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG5243.278073193619018No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT3041.9017829214888957No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC2501.563966218329684No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT1861.163590866437285No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT1280.8007507037847982Illumina PCR Primer Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC970.6068188927119175No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAGCTCGTATGCCGT840.5254926493587738No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT460.28776978417266186RNA PCR Primer, Index 41 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC450.28151391929934316No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG400.25023459493274947No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC330.2064435408195183No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGC300.1876759461995621No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATATCGTATGCCGT290.18142008132624335No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATTTCGTATGCCGT250.15639662183296843No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG230.14388489208633093No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCG230.14388489208633093No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCCCGTATGCCGT220.13762902721301218No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCC160.10009383797309977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACA150.002184786870.0000157
ACTCGTA252.7145888E-670.038
ATAACTC252.7145888E-670.035
AACTCGT252.7145888E-670.037
TAACTCG252.7145888E-670.036
GCCATAA252.7145888E-670.032
CCATAAC252.7145888E-670.033
CCATACC352.7929127E-760.033
ATACCTC352.7929127E-760.035
CATACCT352.7929127E-760.034
CATAACT308.009023E-658.33333634
TTCTGCT7700.056.8181852
TTGAAAA7650.056.7320358
GCCGTCT7650.056.7320346
CTTGAAA7650.056.7320357
CCGTCTT7600.056.6447447
TCTTCTG7600.056.6447450
CTGCTTG7850.056.62420354
CGCCATA8050.056.5217431
CTCGTAT8050.056.5217439
Produced by FastQC (version 0.11.5)